Cytogenetic and cellular characteristics of a human embryonal rhabdomyosarcoma cell line, RMS-YM

Characterization of a New Human Embryonal Rhabdomyosarcoma Cell Line, RMS‐GR

“…Analyses of this cell line, named RMS-GR, indicated that, although several types of cells were seen, small spindle-shaped and rounded or polygonal cells predominated. Similar findings were obtained in other rhabdomyosarcoma cell lines 19,21,25) which showed no morphological features of differentiation under a light microscope. Our cell line did not show the spontaneous cell fusion observed in the line established by Petkovi et al,26) and in contrast with rhabdomyosarcoma cell line TS-RM-1, 21) ultrastructural analysis of RMS-GR cells showed few lipid droplets and glycogen accumulations, features considered by Enzinger and Weiss 1) as signs of differentiation.…”

“…Although cell lines derived from these malignant tumors would be useful experimental models for biological investigation, only a few rhabdomyosarcoma cell lines have been established, and most of them were obtained from childhood tumors, in which anatomopathological and immunohistochemical analyses showed a low degree of differentiation. [17][18][19][20][21] However, some of these lines may be induced to differentiate with pharmacological or biological agents. [22][23][24] We established a new human rhabdomyosarcoma cell line from a primary tumor.…”

Molecular cytogenetic analysis consistently identifies translocations involving chromosomes 1, 2 and 15 in five embryonal rhabdomyosarcoma cell lines and a PAX-FOXO1A fusion gene negative alveolar rhabdomyosarcoma cell line

“…HX170 was established in the Institute of Cancer Research, Sutton, Surrey (Kelland et al, 1989). RMS-YM (Kubo et al, 1991) was kindly provided by Professor Tomoki Naoe.…”

Establishment and Characterization of Canine Rhabdomyosarcoma Cell Line CMS-C

“…In humans, since several RMS cell lines have been established (e.g., Rh28, RMS-YM and RUCH-2), many biological properties of human RMS cells, including specific chromosomal translocations, alterations in molecular pathways and sensitivity to various anticancer drugs, are now understood [4,7,11,15]. It is difficult to analyze these biological properties using biopsy samples or formalinfixed tissues.…”

“…Based on these results, a novel canine RMS cell line was successfully established that retained morphological and immunocytochemical characteristics of canine RMS tumor cells. A number of established cultured cell lines from human RMS have been characterized in various respects including the morphological features, cellular differentiation, cytogenetics, genes and effect of anticancer drugs or radiation [4,7,9,11,15]. The development of a canine RMS cell line may enable us to analyze these characteristics about canine RMS, not only for basic studies but also for clinical studies.…”