Establishment and Characterization of Canine Rhabdomyosarcoma Cell Line CMS-C

Azakami, Daigo; Shibutani, Hana; Dohi, Marie; Takasaki, Mariko; Ishioka, Katsumi; Mori, Akira; Momota, Yutaka; Bonkobara, Makoto; Washizu, Tsukimi; Michishita, Masaki; Hatakeyama, Hitoshi; Ogasawara, Seigo; Sako, Toshinori

doi:10.1292/jvms.10-0436

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…RMS has a moderate metastatic rate, but can spread to the lungs, liver, spleen, kidneys and adrenal glands. Despite low metastatic rate, RMS is locally invasive (Azakami and others 2011). In agreement with the literature (Meuten 2002), we built Table 1 that summarises the main characteristics of the RM and RMS.…”

Section: Discussionmentioning

confidence: 99%

A laryngeal rhabdomyosarcoma in a dog

Sanspoux¹

2016

Vet Record Case Reports

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A six-year-old mongrel dog presented with modified voice, stridor, cough, difficulty swallowing and exercise intolerance while hunting since the last six weeks. The primary consideration was laryngeal paralysis and an endoscopic examination was performed. A 3 cm mass was found on the right arytaenoid cartilage rostral to the vocal cord. Biopsy of the mass revealed a tumour originating from striated muscles. After tumour staging with CT, the mass was removed by a ventral approach to the larynx. Histopathological analysis was compatible with a spindle cell sarcoma and rhabdomyosarcoma was confirmed by immunohistochemistry. No palliative chemotherapy was introduced. The dog was still alive 18 months after surgery.

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Section: Discussionmentioning

confidence: 99%

A laryngeal rhabdomyosarcoma in a dog

Sanspoux¹

2016

Vet Record Case Reports

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“…31 Pleomorphic RMS occurs mostly in adult people, 19,109 and the 2 cases in dogs have been in a 3-year-old and a 14-year-old dog. 8,31 Attempts have been made to segregate neoplasms into different categories for the purpose of making predictions of biologic behavior based on histomorphologic, ultrastructural, or immunohistochemical features. The most recent, and widely used, human classification system is the international classification system developed by Newton et al; 104 however, similar yet differing systems exist and may be encountered periodically in literature searches.…”

Section: Histologic Patternsmentioning

confidence: 99%

“…Although a tumor cell line was established from a pleomorphic RMS from a 14-year-old mixed breed dog, no cytogenetic abnormalities were reported. 8 One report described single or multiple RMS developing in 25 of 100 piglets from multiple litters over a 6-month period in the Netherlands, suggesting an inherited component. 157 van der Loop and colleagues established a cell culture from a pleomorphic RMS in a 3.5-week-old female piglet and described a deletion of the long arm of the X chromosome (Xq24-qter) in these cells.…”

Section: Cytogenetics Of Rms In Domestic Animalsmentioning

confidence: 99%

A Comparative Review of Canine and Human Rhabdomyosarcoma With Emphasis on Classification and Pathogenesis

Caserto

2013

Vet Pathol

144

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Rhabdomyosarcomas are a diverse group of malignant mesenchymal neoplasms exhibiting variable levels of differentiation toward skeletal myocytes. Neoplastic cells may resemble relatively undifferentiated myoblasts, satellite cells, or more differentiated elongated spindle cells and multicellular myotubes. In veterinary medicine, classification into subtypes and variants is based on an outdated system derived from human pathology and is solely based on histologic characteristics. In contrast, classification of human rhabdomyosarcoma is based on histologic, immunohistochemical, and molecular diagnostic techniques, and subclassification has clinical and prognostic relevance. Relevance of tumor subtyping has not been established in veterinary medicine. Recent discoveries of components of the molecular pathogenesis and genomes of human rhabdomyosarcomas have led to new diagnostic techniques and revisions of the human classification system. The current classification system in veterinary medicine is reviewed in light of these changes. Diagnosis of rhabdomyosarcoma using histopathology, electron microscopy, and the clinical aspects of human and canine rhabdomyosarcomas is compared. The clinical features and biologic behavior of canine rhabdomyosarcomas are compared with canine soft tissue sarcomas.

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“…The canine rhabdomyosarcoma cell line CMS-C [ 2 ] was maintained in Dulbecco’s modified eagle medium (DMEM, Wako, Osaka, Japan) with 10% heat-inactivated fetal calf serum (Hyclone, Logan, UT, U.S.A.) and antibiotics (Nakarai tesque, Kyoto, Japan) at 37°C in an atmosphere containing 5% CO 2 . CMS-J, bladder rhabdomyosarcoma from a 14-year-old spayed female beagle dog, was established as reported previously [ 2 ] and maintained in the same medium.…”

Section: Methodsmentioning

confidence: 99%

Identification of tumor-initiating cells derived from two canine rhabdomyosarcoma cell lines

Kishimoto

Yashima

Nakahira

et al. 2017

The Journal of Veterinary Medical Science

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Cancer stem cells or tumor-initiating cells (TICs) are a small subpopulation of cells that have the capacity to self-renew, differentiate and initiate tumors. These cells may function in tumor initiation, aggression and recurrence. Whether spheres derived from canine rhabdomyosarcoma cells have stem cell-like properties is unclear. We induced sphere formation in the canine rhabdomyosarcoma cell lines, CMS-C and CMS-J, and characterized the spheres in vitro and in vivo. Sphere-forming cells were more resistant to vincristine, mitoxantrone and doxorubicin than adherent cells. Xenograft transplantation demonstrated that 1 × 103 sphere-forming cells derived from CMS-C were sufficient for tumor formation. The sphere assay showed that the sphere-forming cells were present in these tumors. These results suggest that the spheres derived from canine rhabdomyosarcoma cells may possess characteristics of TICs. This study provides the foundation for elucidating the contribution of TICs to rhabdomyosarcoma tumorigenesis.

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Establishment and Characterization of Canine Rhabdomyosarcoma Cell Line CMS-C

Cited by 9 publications

References 15 publications

A laryngeal rhabdomyosarcoma in a dog

A laryngeal rhabdomyosarcoma in a dog

A Comparative Review of Canine and Human Rhabdomyosarcoma With Emphasis on Classification and Pathogenesis

Identification of tumor-initiating cells derived from two canine rhabdomyosarcoma cell lines

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