Drug hypersensitivity reactions (DHRs) are a matter of great concern, both for outpatient and in hospital care. The evaluation of these patients is complex, because in vivo tests have a suboptimal sensitivity and can be time-consuming, expensive and potentially risky, especially drug provocation tests. There are several currently available in vitro methods that can be classified into two main groups: those that help to characterize the active phase of the reaction and those that help to identify the culprit drug. The utility of these in vitro methods depends on the mechanisms involved, meaning that they cannot be used for the evaluation of all types of DHRs. Moreover, their effectiveness has not been defined by a consensus agreement between experts in the field. Thus, the European Network on Drug Allergy and Drug Allergy Interest Group of the European Academy of Allergy and Clinical Immunology has organized a task force to provide data and recommendations regarding the available in vitro methods for DHR diagnosis. We have found that although there are many in vitro tests, few of them can be given a recommendation of grade B or above mainly because there is a lack of well-controlled studies, most information comes from small studies with few subjects and results are not always confirmed in later studies. Therefore, it is necessary to validate the currently available in vitro tests in a large series of well-characterized patients with DHR and to develop new tests for diagnosis.

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In the 20th century, in the Mediterranean area, many extensive afforestation efforts were made with the primary objective of protecting soils from erosion and improving their fertility. This study evaluates the effects of the afforestation undertaken in the Guadalentín basin (SE Spain) with respect to the organic and inorganic soil constituents and physico-chemical soil properties. Given the phytoclimatic environments in the basin (sclerophyllous and hyperxerophyllous), paired samples were taken beneath the tree canopy of the pine plantations and in nearby open zones. With the same methodology, samples were taken from areas considered to be native forest. The data were submitted to different multivariate analyses of variance (two-way MANOVAs) in order to compare the effects and interactions of the factors CANOPY (with and without trees), PHYTOCLIMATE (sclerophyllous and hyperxerophyllous), and TYPE OF FOREST (afforested or native) on the dependent variables measured (soil variables). Significant differences were found at 0-10 cm in soil depth under pine afforestations in relation to adjacent open areas. Below this depth, differences were found only between phytoclimatic environments. No significant interactions were found between the variables analysed at any of the depths, indicating that the effects of the afforestations on the soil characteristics were independent of the phytoclimatic environment. The afforestation in the Guadalentín basin, in the two phytoclimatic environments considered increased the soil fertility. Nevertheless, the native forests presented the highest soil organic-carbon contents, mainly in the sclerophyllous phytoclimate type (Quercus ilex subsp. ballota forests). Therefore, although the afforestations improved the soil fertility in relation to the open areas, the maximum potential has probably not been reached in relation to that observed in the native forests. The effects that forest development (age, basal area) over time exerts on soil properties remain to be verified by further research.