CYLD expression in dendritic cells involved in the immunoregulation of pulmonary adenocarcinoma via NF-κB pathway

Yu, Qinghua; Lei, Yujie; Huang, Yunchao; Zhang, Ji‐Guang; Chen, Yangming; Chen, Kai; Lin, Jianbin; Sun, Shenghong; Lin, Xiaolan

doi:10.1080/21691401.2019.1699820

Cited by 6 publications

(7 citation statements)

References 34 publications

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“…Our results indicated that CYLD-knockout DCs show decreased signaling and nuclear translocation of NF-kB. Inhibition of the NF-kB pathway inhibits the DCs-induced proliferation and function of CD4 + T cells, indicating that CYLD exerts a positive effect on T-cell function [9] . In this study, DCs were transfected with a miR-124-3p mimic or miR-124-3p inhibitor, and were then co-cultured with CD4 + T cells.…”

Section: Discussionmentioning

confidence: 61%

“…Our previous studies on the distribution and differential expression of CYLD and DCs in lung cancer tissues and corresponding adjacent tissues showed that CYLD expression is correlated with the distribution of mDCs in lung cancer and adjacent tissues. Those studies showed that increased expression levels of the CYLD protein in lungcancer tissues are correlated with increased rate of DCs maturation [9] . miRNAs play important roles in regulating gene expression in tumor biology [10] .…”

Section: Discussionmentioning

confidence: 99%

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miR-124-3p enhances dendritic cell-mediated anti-tumor immunity by targeting CYLD/4-1BBL pathway

Lei¹,

Chen

et al. 2021

Preprint

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Objective: To investigate miR-124-3p regulation of DC-mediated immune response via CYLD/4-1BBL pathway, and the inhibitory effect of miR-124-3p on lung cancer.Methods: DCs were cultured and amplified in vitro, and then transfected with miR-124-3p mimic, miR-124-3P inhibitor, or siRNA CYLD. Double luciferase reporter genes were used to detect the target relationship between miR-124-3p and CYLD. qRT-PCR and western blotting were used to detect the expression levels of CYLD and 4-1BBL. Flow cytometry was used to assess the proliferation rate of CD4+ T cells co-cultured with untransfected DCs and those transfected with miR-124-3p mimic or miR-124-3p inhibitor. C57BL/6 tumor bearing mice, implanted with LL/2 lung adenocarcinoma cells, were administered DCs transfected with the miR-124-3p mimic or untransfected DCs. The tumor size and weight of mice were then measured. Results: miR-124-3p and CYLD3’UTR contained target-binding site, and overexpression of miR-124-3p enhanced the expression of CYLD and 4-1BBL. CD4+ T cells co-cultured with miR-124-3p mimic-transfected DCs showed significantly increased proliferation. In tumor-bearing mice, tumor inhibition rate was 73.5%, and tumor volume and weight were significantly decreased after the administration of DCs containing the miR-124-3p mimic.Conclusions: The expression of CYLD was regulated by miR-124-3p, which, in turn, increased the expression of 4-1BBL. miR-124-3p regulated DCs function via CYLD/4-1BBL cascade. miR-124-3p plays important roles in DCs-induced T cells, thereby enhancing anti-tumor immunity.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

miR-124-3p enhances dendritic cell-mediated anti-tumor immunity by targeting CYLD/4-1BBL pathway

Lei¹,

Chen

et al. 2021

Preprint

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“…DCs play a key role in tumor immunotherapy, and relevant research is ongoing. CYLD is a deubiquitination enzyme that regulates various signaling pathways in physiological processes (9). Our previous studies on the distribution and differential expression of CYLD and DCs in lung cancer tissues and corresponding adjacent tissues showed that CYLD expression is correlated with the distribution of mDCs in lung cancer and adjacent tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies on the distribution and differential expression of CYLD and DCs in lung cancer tissues and corresponding adjacent tissues showed that CYLD expression is correlated with the distribution of mDCs in lung cancer and adjacent tissues. Those studies showed that increased expression levels of the CYLD protein in lung-cancer tissues are correlated with increased rate of DCs maturation (9).…”

Section: Discussionmentioning

confidence: 99%

miR-124-3p enhances dendritic cell-mediated anti-tumor immunity by targeting CYLD/4-1BBL pathway

Lei

Chen

et al. 2021

Preprint

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Background: MicroRNAs play important roles in dendritic cell (DCs)-mediated immunity, but their specific functions in lung cancer remain unclear.Objective: To investigate miR-124-3p regulation of DC-mediated immune response via CYLD/4-1BBL pathway, and the inhibitory effect of miR-124-3p on lung cancer.Methods: DCs were cultured and amplified in vitro, and then transfected with miR-124-3p mimic, miR-124-3P inhibitor, or siRNA CYLD. Double luciferase reporter genes were used to detect the target relationship between miR-124-3p and CYLD. qRT-PCR and western blotting were used to detect the expression levels of CYLD and 4-1BBL. Flow cytometry was used to assess the proliferation rate of CD4+ T cells co-cultured with untransfected DCs and those transfected with miR-124-3p mimic or miR-124-3p inhibitor. C57BL/6 tumor bearing mice, implanted with LL/2 lung adenocarcinoma cells, were administered DCs transfected with the miR-124-3p mimic or untransfected DCs. The tumor size and weight of mice were then measured. Results: miR-124-3p and CYLD3’UTR contained target-binding site, and overexpression of miR-124-3p enhanced the expression of CYLD and 4-1BBL. CD4+ T cells co-cultured with miR-124-3p mimic-transfected DCs showed significantly increased proliferation. In tumor-bearing mice, tumor inhibition rate was 73.5%, and tumor volume and weight were significantly decreased after the administration of DCs containing the miR-124-3p mimic.Conclusions: The expression of CYLD was regulated by miR-124-3p, which, in turn, increased the expression of 4-1BBL. miR-124-3p regulated DCs function via CYLD/4-1BBL cascade. miR-124-3p plays important roles in DCs-induced T cells, thereby enhancing anti-tumor immunity.

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“…Immune genes of LUAD brain metastasis were involved in the adaptive immune response, B cell differentiation, myeloid leukocyte differentiation, cellular extravasation, positive regulation of leukocyte migration, interleukin-12 production, activation of immune response, regulation of inflammatory response, regulation of cytokine production, leukocyte migration, leukocyte activation involved in immune response, leukocyte differentiation, and other biological functions, and might be involved in the regulation of cellular senescence, c-type lectin receptor signaling pathway, natural killer cell mediated cytotoxicity, CAMs, primary immunodeficiency, and NF-κB signaling pathway, among others. Studies have reported that the adaptive immune response, the NF-κB signaling pathway, and the IL12 signaling mechanism, among others, play important roles in the progression of LUAD (17)(18)(19)(20). For example, IL33 inhibited the growth of LUAD by regulating dendritic cells (DCs).…”

Section: Discussionmentioning

confidence: 99%

Screening and evaluation of the role of immune genes of brain metastasis in lung adenocarcinoma progression based on the TCGA and GEO databases

Chen¹,

Guo²,

Tang³

et al. 2021

J Thorac Dis

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Background: Brain metastasis was one of the factors leading to the poor long-term prognosis of patients with lung adenocarcinoma (LUAD).Methods: The expression levels of immune genes in LUAD and LUAD brain metastases tissues were analyzed in GSE161116 dataset using the GEO2R, and the levels of differential immune genes in normal lung and LUAD tissues were verified. The biological functions and signaling mechanisms of the differential immune genes were explored via Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis.Cox regression analysis was used to screen the prognostic factors of LUAD patients, and a risk model was constructed. The role of the model was checked in the development of LUAD via receiver operating characteristic analysis, gene set enrichment analysis, and Cox regression analysis.Results: Differentially expressed genes (DEGs) in brain metastasis were involved in the adaptive immune response, B cell differentiation, leukocyte migration, NF-kB signaling pathway, among others. The expression levels of TNFRSF11A, MS4A2, IL11, CAMP, MS4A1, and F2RL1 were independent factors affecting the poor prognosis of LUAD patients via Cox regression analysis and Akaike information criterion.In the constructed risk model, the overall survival of LUAD patients in the high-risk group was poor. The risk model was significantly related to the gender, clinical stage, T stage, lymph node metastasis, and survival status of LUAD patients. In addition, the risk model score was an independent risk factor that affected the poor prognosis of LUAD patients. TNFRSF11A, CAMP, F2RL1, IL11, MS4A1, and MS4A2 of the risk factors had diagnostic significance in LUAD brain metastasis and LUAD. The risk model participated in cytokinetic process, cell cycle, citrate cycle TCA cycle, etc. The risk model score was correlated with the levels of B cells memory, mast cells resting, macrophages M0, mast cells activated, neutrophils, eosinophils, T cells gamma delta, and immune cell markers. Conclusions:The risk model based on the LUAD brain metastasis immune factors TNFRSF11A, MS4A2, IL11, CAMP, MS4A1, and F2RL1 was related to the diagnosis, poor prognosis, and immune infiltrating cells of LUAD patients, and is expected to provide a reference for the development of treatment strategies for LUAD patients.

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CYLD expression in dendritic cells involved in the immunoregulation of pulmonary adenocarcinoma via NF-κB pathway

Abstract: View related articlesView Crossmark data Citing articles: 1 View citing articles CYLD expression in dendritic cells involved in the immunoregulation of pulmonary adenocarcinoma via NF-jB pathway

Cited by 6 publications

References 34 publications

miR-124-3p enhances dendritic cell-mediated anti-tumor immunity by targeting CYLD/4-1BBL pathway

miR-124-3p enhances dendritic cell-mediated anti-tumor immunity by targeting CYLD/4-1BBL pathway

miR-124-3p enhances dendritic cell-mediated anti-tumor immunity by targeting CYLD/4-1BBL pathway

Screening and evaluation of the role of immune genes of brain metastasis in lung adenocarcinoma progression based on the TCGA and GEO databases

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