Curcumin analogue inhibits lipid peroxidation in a freshwater teleost, Anabas testudineus (Bloch)—an in vitro and in vivo study

Abstract

The effect of a synthetic curcumin analogue (salicylcurcumin) on fish lipid peroxidation was investigated in both in vitro and in vivo conditions using a teleost model Anabas testudineus (Bloch). Curcumin analogue inhibited the formation of lipid peroxidation products and thiobarbituric acid reactive substances (TBARS) content at the three concentrations (10(-2) M, 10(-3) M and 10(-4) M) in vitro. TBARS content was reduced by 80% in the liver and 68% in brain by the higher concentration of salicylcurcumin. For in vivo study, salicylcurcumin (0.5%) was supplemented along with the basal feed for a period of 60 days. It produced a 60% reduction in liver TBARS content. The antioxidant enzyme superoxide dismutase (SOD) was stimulated, whereas catalase (CAT) and glutathione peroxidase (GPx) were inhibited. Glutathione (GSH) was reduced and glutathione reductase (GR) unchanged. Even though there was an increase in SOD activity, the CAT and GPx did not increase accordingly, maybe due to the direct scavenging of H(2)O(2) by salicylcurcumin. The protein content also increased in the curcumin-fed animals, indicating a positive growth-promoting effect. Therefore, it would be beneficial to supplement salicylcurcumin along with the aquaculture feed in order to help the fish to cope with adverse conditions in the environment. This would increase the survival rate, disease resistance and ultimately the growth rate.

Effects of dietary curcumin on growth, antioxidant capacity, fatty acid composition and expression of lipid metabolism-related genes of large yellow croaker fed a high-fat diet

“…Furthermore, this study also found that dietary curcumin increased T-AOC and the activity of SOD and CAT, which are crucial parameters in antioxidant system. Similar results were found in climbing perch and rainbow trout that dietary curcumin increased activity of SOD and/or CAT (20,37) . Collectively, these indicated that curcumin supplementation was likely to improve the health and function of the piscine liver through alleviating liver damage and improving antioxidant capacity.…”

“…Suitable feed additives are effective to alleviate the adverse effects of HFD in farmed fish. The recent studies have found that dietary curcumin supplementation increased protein content and inhibited lipid peroxidation in the climbing perch liver (37,38) . In other fish, 50 mg/kg curcumin significantly improved the growth performance of tilapia fed the standard diet (20) .…”

Mechanochemical Synthesis and Antioxidant Activity of Curcumin‐Templated Azoles

“…The reported methods for synthesizing these curcumin‐templated azoles generally involve long reaction times. With our interest in the synthesis and bioactivity studies on curcumin, its analogs and derivatives , we continue to investigate the structural modification of curcumin at its β‐diketone unit by transforming this central part of the molecule into an isosteric heterocycle. Thus, we now report a rapid, solvent‐free mechanochemical route to these bis(styryl)pyrazoles and isoxazole.…”

In vivo protective effect of dietary curcumin in fish Anabas testudineus (Bloch)

“…Studies on the effect of curcumin on fish tissue are scanty except for a recent report by D' Souza and Prabhu (2006) that turmeric in vitro inhibited lipid peroxidation in Somberus sombrus. Lately, in vitro hepatoprotective effect of various natural curcuminoids in fish Anabas testudineus (Manju et al 2008a) and an in vivo and in vitro protective effect of a synthetic curcumin (salicylcurcumin) (Manju et al 2008b) in the same fish were investigated in our laboratory. Despite reported benefits, in vitro studies in cultured cells have demonstrated that curcumin induces chromosomal and DNA damage (Antunes et al 1999;Araujo et al 1999a, b) detected by the comet assay (Kelly et al 2001;Urbina-Cano et al 2006).…”