Crystalline arrays of spherical particles in turnip yellow mosaic virus-infected cells

Rubio-Huertos, M.; Vela, A; López‐Abella, D.

doi:10.1016/0042-6822(67)90295-4

Cited by 17 publications

(4 citation statements)

References 5 publications

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“…Subsequently, carbon replicas of natural TYMV microcrystals were obtained (Cornuet, 1959) and within a decade osmiophilic arrays of TYMV were observed in the leaves of Chinese cabbage (Rubio-Huertos et al, 1967). Negatively stained ultra-thin sections of leaves of inoculated oat or barley also revealed crystalline arrangements of BMV (Paliwal, 1970).…”

Section: In Vitro-produced Versus Naturally Occurring Virus Crystalsmentioning

confidence: 99%

“…Loose arrays of 1 Â 0.5 mm 2 , $400 virions ($800 v./mm 2 ) with a hexagonal pattern in some regions found in chloroplast, cytoplasm and X-bodies in Chinese cabbage (Rubio-Huertos et al, 1967). a Maximal size measured on cryo-electron microscope grids prepared as described under Section 2. b The symmetry depends on the plane along which the lattice is cleaved.…”

Section: Tymvmentioning

confidence: 99%

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Formation of two-dimensional crystals of icosahedral RNA viruses

Lorber

Adrian

Witz

et al. 2008

Micron

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Section: In Vitro-produced Versus Naturally Occurring Virus Crystalsmentioning

confidence: 99%

Section: Tymvmentioning

confidence: 99%

Formation of two-dimensional crystals of icosahedral RNA viruses

Lorber

Adrian

Witz

et al. 2008

Micron

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“…1a, is one of the most thoroughly studied of all viruses (Markham and Smith, 1949;Matthews, 1991;Rubio-Huertos et al, 1967;Finch and Klug, 1966;Hirth and Givord, 1988;Mellema and Amos, 1972). The TYMV genome is made up of an M r ϭ 1.9 ϫ 10 6 , single-stranded RNA of 6218 bases (Hirth and Givord, 1988) and a 694-nucleotide subgenomic RNA that is included in most virions.…”

Section: Methodsmentioning

confidence: 99%

Surface Processes in the Crystallization of Turnip Yellow Mosaic Virus Visualized by Atomic Force Microscopy

Malkin

Kuznetsov

Lucas

et al. 1999

Journal of Structural Biology

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In situ atomic force microscopy (AFM) was used to investigate surface evolution during the growth of single crystals of turnip yellow mosaic virus (TYMV). Growth of the (101) face of TYMV crystals proceeded by two-dimensional nucleation. The molecular structure of the step edges and adsorption of individual virus particles and their aggregates on the crystalline surface were recorded. The surfaces of individual virions within crystals were visualized and seen to be quite distinctive with the hexameric and pentameric capsomers of the T ‫؍‬ 3 capsids being clearly resolved. This, so far as we are aware, is the first direct visualization of the capsomere structure of a virus by AFM. In the course of recording the in situ development of the crystals, a profound restructuring of the surface arrangement was observed. This transformation was highly cooperative in nature, but the transitions were unambiguous and readily explicable in terms of an organized loss of classes of virus particles from specific lattice positions. In some cases areas of a single crystal surface were recorded in which were captured successive phases of the transition. We believe this provides the first visual record of a cooperative restructuring of the surface of a supramolecular crystal. 1999 Academic Press

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“…Alterations in chloroplast structure in several virus-infected plant species displaying chlorotic symptoms have been reported. In turnip yellow mosaic virus (TYMV)-infected Chinese cabbage, the chloroplasts in the yellow areas of the leaves are often rounded, have few grana, are devoid of starch grains and eventually lose their identity, while those in the green areas are apparently 192 i>A GRA^A and MARTIN normal (CHALCROFT andMATTHEWS 1966, RUBIO-HUERTOS, VELA andLOPEZ-ABELLA 1967). These observations are very similar to those presented here for sunblotch-infected avocado, except total loss of organelle identity was not detected.…”

Section: Discussionmentioning

confidence: 99%