Formation of two-dimensional crystals of icosahedral RNA viruses

Lorber, Bernard; Adrian, Marc; Witz, Jean‐François; Erhardt, Mathieu; Harris, J. Robin

doi:10.1016/j.micron.2007.02.008

Cited by 11 publications

(12 citation statements)

References 59 publications

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“…These two crystal structures are distinct from the previously reported 2D assemblies of TYMV, i.e. , hexagonal arrays 22,23,27 and the EM-based square crystal, 25–27 whose (

\sqrt{2} \times \sqrt{2}

) unit cell contains 2 particles (Fig. 6B).…”

Section: Resultscontrasting

confidence: 89%

“…Significantly, the two 2D crystal phases observed in the present study are distinct also from the previously reported hexagonal 22,23,27 and centered square 25–27 lattices of TYMV. The existence of these multiple 2D phases for TYMV illustrates that a rich variety of ordered packing motifs may be possible for icosahedral particles, even as a single-component system in 2D.…”

Section: Discussioncontrasting

confidence: 59%

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Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

et al. 2013

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We investigate two-dimensional (2D) assembly of the icosahedral turnip yellow mosaic virus (TYMV) under cationic lipid monolayers at the aqueous solution–vapor interface. The 2D crystallization of TYMV has been achieved by enhancing electrostatically induced interfacial adsorption, an approach recently demonstrated for another virus. In situ X-ray scattering reveals two close-packed 2D crystalline phases of TYMV that are distinct from the previously reported hexagonal and centered square ( 2×2) arrays of TYMV. One of the newly observed phases arises from either a dimeric double-square (2 × 1) or tetrameric square (2 × 2) unit cell. The other is a rhombic crystal with a lattice angle of 80°. The two observed crystal phases are substantially less dense (by over10%) than a 2D lattice of TYMV could be according to its known size and shape, indicating that local anisotropic interparticle interactions play a key role in stabilizing these crystals. TYMV’s anisotropy attributes and numerical analysis of 2D arrays of virus-shaped particles are used to derive a model for the rhombic crystal in which the particle orientation is consistent with the electrostatic lipid–TYMV attraction and the interparticle contacts exhibit steric complementarity. The interplay between particle anisotropy and packing is contrasted between the rhombic crystal model and the square ( 2×2) crystal. This study highlights how the high symmetry and subtle asphericity of icosahedral particles enrich the variety and complexity of ordered 2D structures that can be generated through self-assembly.

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“…These two crystal structures are distinct from the previously reported 2D assemblies of TYMV, i.e. , hexagonal arrays 22,23,27 and the EM-based square crystal, 25–27 whose (

\sqrt{2} \times \sqrt{2}

) unit cell contains 2 particles (Fig. 6B).…”

Section: Resultscontrasting

confidence: 89%

Section: Discussioncontrasting

confidence: 59%

Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

et al. 2013

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“…1b). Their capsids are made of 180 copies of protein subunits, with a mass of 40 kDa for TBSV and 20 kDa for TYMV 32 . The virions have diameters of 33 and 28 nm, molecular weights of 9.0 Â 10 3 and 5.5 Â 10 3 kDa, and isoelectric points of 4.1 and 3.8, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Nanopure water (resistivity Z18 MO cm) was produced with a Millipore Synergy purification system. TBSV and TYMVwere propagated and purified as described previously 32 . Filtered-sterilized human serum was purchased from Sigma-Aldrich.…”

Section: Methodsmentioning

confidence: 99%

A synthetic nanomaterial for virus recognition produced by surface imprinting

et al. 2013

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Major stumbling blocks in the production of fully synthetic materials designed to feature virus recognition properties are that the target is large and its self-assembled architecture is fragile. Here we describe a synthetic strategy to produce organic/inorganic nanoparticulate hybrids that recognize non-enveloped icosahedral viruses in water at concentrations down to the picomolar range. We demonstrate that these systems bind a virus that, in turn, acts as a template during the nanomaterial synthesis. These virus imprinted particles then display remarkable selectivity and affinity. The reported method, which is based on surface imprinting using silica nanoparticles that act as a carrier material and organosilanes serving as biomimetic building blocks, goes beyond simple shape imprinting. We demonstrate the formation of a chemical imprint, comparable to the formation of biosilica, due to the template effect of the virion surface on the synthesis of the recognition material.

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“…This may be interpreted as protein-protein interaction becoming dominant by decreasing solute-solute repulsion, as observed for protein solutions near their isoelectric point. The combination of ions from the staining solution as well as the presence of PEG may facilitate this condition, inducing pseudo-crystal formation of larger size as compared to the same solutions, but in the total absence of PEG ( see Lorber et al, 2008). …”

Section: Cryo-negative Staining: Techniques Results and Discussionmentioning

confidence: 99%

Negative staining and cryo-negative staining of macromolecules and viruses for TEM

Carlo

Harris²

2011

Micron

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In this review we cover the technical background to negative staining of biomolecules and viruses, and then expand upon the different possibilities and limitations. Topics range from conventional air-dry negative staining of samples adsorbed to carbon support films, the variant termed the "negative staining-carbon film" technique and negative staining of samples spread across the holes of holey carbon support films, to a consideration of dynamic/time-dependent negative staining. For each of these approaches examples of attainable data are given. The cryo-negative staining technique for the specimen preparation of frozen-hydrated/vitrified samples is also presented. A detailed protocol to successfully achieve cryo-negative staining with ammonium molybdate is given, as well as examples of data, which support the claim that cryo-negative staining provides a useful approach for the high-resolution study of macromolecular and viral structure.

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Formation of two-dimensional crystals of icosahedral RNA viruses

Cited by 11 publications

References 59 publications

Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

Crystallization, structural diversity and anisotropy effects in 2D arrays of icosahedral viruses

A synthetic nanomaterial for virus recognition produced by surface imprinting

Negative staining and cryo-negative staining of macromolecules and viruses for TEM

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