SUMMARYSatellite RNAs of CARNA 5s (cucumber mosaic virus-associated RNA 5s) emerged in serial passage lines of Spanish cucumber mosaic virus isolates established in Chenopodium, squash and two tobacco varieties. No satellite was detected in virus obtained from tomato serial passage lines. The satellites were identified by their biological effect on tomato, by their gel electrophoretic mobility and by nucleic acid hybridization, as being very similar in sequence to known CARNA 5s. Interactions among host, virus and CARNA 5 affect the selection of a particular CARNA 5 variant.
In epidermal strips of pea plants with the Wisconsin strain of the pea streak virus, studied from about three weeks after inoculation, extensive parts of the cytoplasm stained red when treated with phloxine and methylene blue. Usually the stained areas were not sharply delimited. Sometimes more or less well-defined granular and often vacuolated inclusion bodies were observed. They were mainly located near the nucleus and along the cell wall.When sap of such plants was studied in the electron microscope, either negatively stained or shadow-cast, it contained very large amounts of elongate rigid particles typical of the virus. The particles were often found attached to cell organelles.In ultrathin sections the virus particles could be easily detected occurring separately or in bundles of one particle long in the cytoplasm. Several of such bundles frequently occurred together and they were usually attached to membranes, e.g. around vacuoles, like other members of the carlavirus group (potato virus S group).
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