Light and electron microscopy of cytoplasmic and unusual nuclear inclusion bodies evoked by a virus from necrotic peas

Bos, L.; Rubio-Huertos, M.

doi:10.1016/0042-6822(69)90221-9

Cited by 15 publications

(9 citation statements)

References 9 publications

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“…The dense bodies usually lacked discrete morphology, but sometimes consisted of faint crystalline striations. Similar bodies, visible by both light and electron microscopy, were observed in the nuclei of peas infected by pea necrosis virus (3).…”

Section: Comparative Cytopathology Of 11 Psbmv Isolates In Infected Pmentioning

confidence: 85%

Ultrastructural cytology of pea leaves and roots infected by pea seedborne mosaic virus

Hampton

Phillips

Knesek

et al. 1973

Archiv f Virusforschung

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Section: Comparative Cytopathology Of 11 Psbmv Isolates In Infected Pmentioning

confidence: 85%

Ultrastructural cytology of pea leaves and roots infected by pea seedborne mosaic virus

Hampton

Phillips

Knesek

et al. 1973

Archiv f Virusforschung

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“…Further data on techniques and some preliminary results as well as details of inclusions of E 178 have been published already (Bos, 1969a;Bos and Rubio-Huertos, 1969).…”

Section: Inclusion Bodiesmentioning

confidence: 96%

“…Several such necrosis diseases have been recorded under the somewhat confusing name "pea streak", Symptomatologically, the majority are indistinguishable, but some eight different viruses with distinct epidemiologies have been reported as their incitants. The new necrosis disease was soon found to differ from all eight (Bos, 1969b) and to be characterized by most unusual inclusion bodies (Bos, 1969a;Bos and Rubio-Huertos, 1969).…”

Section: Fig 2 Pisum Sativum 'Koroza' 14 (Links) En 24 Dagen (Rechtmentioning

confidence: 97%

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The identification of three new viruses isolated from Wisteria and Pisum in The Netherlands, and the problem of variation within the potato virus Y group

Bos¹

1970

Netherlands Journal of Plant Pathology

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114

105

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Three new legume diseases in The Netherlands are described: Wisteria vein mosaic, pea necrosis, and pea leafroll mosaic. In particle size and morphology and in host reaction the virus isolates resembled bean yellow mosaic virus (BYMV), but they were readily distinguishable in several test plants.In recent years several new legume viruses related to BYMV and bean common mosaic virus have been described. Besides, more and more viruses of the potato virus Y group are proving to be naturally infectious to legumes, e.g. lettuce mosaic virus, beet mosaic virus, watermelon mosaic virus, and even turnip mosaic virus, all of which are somehow related to BYMV. To investigate the nature and degree of these relationships, the virus isolates causing the three new legume diseases were compared with a normal strain of BYMV and with pea mosaic virus, clover yellow vein virus, cowpea aphid. borne mosaic virus, two isolates of beet mosaic virus, and lettuce mosaic virus.They were all found to have several hosts and symptoms in common. The differences observed showed a range of gradations only. Unexpectedly, BYMV was found to infect 17 out of 20 non-legumes tested. The Wisteria isolate and lettuce mosaic virus did not produce inclusion bodies, whereas all others did. Often nueleoli were very much enlarged or contained crystals. The pea necrosis isolate produced many nucleolar crystalline needles.Cross-protection tests were of little help in determining mutual relationships.Antisera prepared against the Wisteria isolate, the pea necrosis isolate, and BYMV, and an antiserum against bean common mosaic virus, revealed definite relationships, but also substantial differences. By using the electron microscope the three new isolates were indistinguishable from BYMV, whereas the particle lengths of two isolates of beet mosaic virus were considerably shorter. The isolate of pea mosaic virus had much longer (840 m~x) and more rigid particles.

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“…In his review, however, nucleolar abnormalities were not taken into account. All CYV¥-like isolates studied showed the highly characterisitic nucleolar enlargements earlier described [7,10,11]. With E178 the enlarged nucleolus is usually covered with long needles often penetrating through the nuclear membrane [11].…”

Section: Discussionmentioning

confidence: 97%

Identification of potyvirus isolates from faba bean (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus

Fortass¹,

Bos²,

Goldbach³

1991

Archives of Virology

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Clover yellow vein virus (CYVV) isolate H and the related potyvirus isolates E178, E197, and E242 could be distinguished from bean yellow mosaic (BYMV) isolates by their wider host range among non-legume test plant species, the peculiar enlargement of the nucleolus in infected plants, and the larger size of their coat protein as evidenced by slower migration in SDS-PAGE. Serologically, they are qualitatively indistinguishable in electro-blot immunoassay (EBIA) also with antibodies specific to the N-terminal part of BYMV-B25 coat protein, implying therefore that CYVV and BYMV coat proteins contain identical amino acid sequences in the N-terminal region. The faba-bean virus isolates from Sudan, Syria, and The Netherlands could be identified as BYMV isolates especially adapted to faba bean. All of them were weakly pathogenic to Phaseolus bean with the exception of SV205, assuming an intermediate position between Phaseolus-bean isolates, with low pathogenicity to faba bean, and faba-bean isolates, usually having low pathogenicity to Phaseolus bean. Strains of BYMV are thus hard to delimit.

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Light and electron microscopy of cytoplasmic and unusual nuclear inclusion bodies evoked by a virus from necrotic peas

Cited by 15 publications

References 9 publications

Ultrastructural cytology of pea leaves and roots infected by pea seedborne mosaic virus

Ultrastructural cytology of pea leaves and roots infected by pea seedborne mosaic virus

The identification of three new viruses isolated from Wisteria and Pisum in The Netherlands, and the problem of variation within the potato virus Y group

Identification of potyvirus isolates from faba bean (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus

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