Emergence and Characterization of Satellite RNAs Associated with Spanish Cucumber Mosaic Virus Isolates

García-Luque, Isabel; Kaper, J.M.; Díaz-Ruíz, J. R.; Rubio-Huertos, M.

doi:10.1099/0022-1317-65-3-539

Cited by 29 publications

(13 citation statements)

References 11 publications

(9 reference statements)

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“…1993b). In natural mixtures, the nature of the dominating variant changed as a result of passage in different hosts (GARCiA-LuQUE et al 1984;KAPER et al 1988;KURATH and PALUKAITIS 1989a). Such an effcct has been dcmonstratcd in the greenhouse, during linear passaging 01' CMV containing a non-pathogenic satcllitc RNA, where a single nucleotide change in the satellitc RNA rendcrcd thc satcllitc RNA nonpathogenic (PALUKAITIS and ROOSSINCK 1996).…”

Section: Satellite Biocontrolmentioning

confidence: 99%

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

García‐Arenal

Palukaitis²

1999

Current Topics in Microbiology and Immunology

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Section: Satellite Biocontrolmentioning

confidence: 99%

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

García‐Arenal

Palukaitis²

1999

Current Topics in Microbiology and Immunology

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“…Aphid cultures, virus sources and test plants were generated and maintained according to Fereres et al (1993). The virus strains used were : a pepper-PVY isolate (pathotype 0) inoculated on ' Yolo Wonder ' pepper plants and the Val-CMV-24 isolate described by Garcia-Luque et al (1984). All aphids were subjected to a 1 h pre-acquisition starvation period before the beginning of the experiments.…”

mentioning

confidence: 99%

Intracellular ingestion and salivation by aphids may cause the acquisition and inoculation of non-persistently transmitted plant viruses.

Martin

Collar²,

Tjallingii³

et al. 1997

Journal of General Virology

327

317

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Transmission of non-persistent plant viruses is related to aphid behaviour during superficial brief probes. A widely accepted hypothesis postulates that virus acquisition occurs during ingestion of plant cell contents, and inoculation during egestion or regurgitation of previously ingested sap. Although conceptually attractive, this ingestionegestion hypothesis has not been clearly demonstrated. Furthermore, it overlooks the anatomy of the tips of the stylets (mouthparts) and, consequently, the potential role of salivation in the inoculation process. Here, we used the electrical penetration graph (EPG) technique to investigate aphid-stylet activities associated with uptake (acquisition) and release (inoculation) of two nonpersistently transmitted viruses. Our results show that acquisition occurs primarily during the last sub-phase (II-3) of intracellular stylet punctures, whereas inoculation is achieved during the first sub-phase (II-1). An alternative mechanism to the ingestion-egestion hypothesis is proposed on the basis of our findings.The transmission of non-persistent plant viruses is unique to aphids (Homoptera : Aphididae) because they exhibit specific and characteristic activities during brief (a few seconds or minutes) and superficial probes, involved in host plant recognition (Pollard, 1973). Two different hypotheses have been proposed to explain the mechanism of transmission. The first, so-called stylet-borne hypothesis (Kennedy et al., 1962),

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“…Seventeen CMV-satRNA isolates were randomly sampled out of 62 field isolates collected during the springs of , 1990and described in Aranda et al (1993. The isolates (from where these 17 were sampled) each represented an electrophoretic variant in semidenaturing polyacrylamide electrophoresis (as in Aranda et al 1993;García-Luque et al 1984) present in virion RNA directly purified from field-infected tomato plants. CMV-satRNA variants were named N/i.j, meaning electrophoretic variant j from field sample i obtained in year N (Aranda et al 1993).…”

Section: Methodsmentioning

confidence: 99%

Contribution of Mutation and RNA Recombination to the Evolution of a Plant Pathogenic RNA

et al. 1997

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The nucleotide sequence of 17 variants of the satellite RNA of cucumber mosaic virus (CMV-satRNA) isolated from field-infected tomato plants in the springs of 1989, 1990, and 1991 was determined. The sequence of each of the 17 satRNAs was unique and was between 334 and 340 nucleotides in length; 57 positions were polymorphic. There was much genetic divergence, ranging from 0.006 to 0.141 nucleotide substitutions per site for pairwise comparisons, and averaging 0.074 for any pair. When the polymorphic positions were analyzed relative to a secondary structure model proposed for CMV-satRNAs, it was found that there were significantly different numbers of changes in base-paired and non-base-paired positions, and that mutations that did not disrupt base pairing were preferred at the putatively paired sites. This supports the concept that the need to maintain a functional structure may limit genetic divergence of CMV-satRNA. Phylogenetic analyses showed that the 17 CMV-satRNA variants clustered into two subgroups, I and II, and evolutionary lines proceeding by the sequential accumulation of mutations were apparent. Three satRNA variants were outliers for these two phylogenetic groups. They were shown to be recombinants of subgroup I and II satRNAs by calculating phylogenies for different molecular regions and by using Sawyer's test for gene conversion. At least two recombination events were required to produce these three recombinant satRNAs. Thus, recombinants were found to be frequent ( approximately 17%) in natural populations of CMV-satRNA, and recombination may make an important contribution to the generation of new variants. To our knowledge this is the first report of data allowing the frequency of recombinant isolates in natural populations of an RNA replicon to be estimated.

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Emergence and Characterization of Satellite RNAs Associated with Spanish Cucumber Mosaic Virus Isolates

Cited by 29 publications

References 11 publications

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

Structure and Functional Relationships of Satellite RNAs of Cucumber Mosaic Virus

Intracellular ingestion and salivation by aphids may cause the acquisition and inoculation of non-persistently transmitted plant viruses.

Contribution of Mutation and RNA Recombination to the Evolution of a Plant Pathogenic RNA

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