Coumarin 6 and 1,6-diphenyl-1,3,5-hexatriene (DPH) as fluorescent probes to monitor protein aggregation

Makwana, Pinakin K.; Jethva, Prashant N.; Roy, Ipsita

doi:10.1039/c0an00829j

Cited by 28 publications

(25 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To validate this, an apolar-sensitive probe, 1,6-diphenyl-1,3,5-hexatriene (DPH, λ em = 428 nm), was anchored on the vesicular wall to detect the change of hydrophobic region. [20] As expected, the fluorescence produced a dramatic jump over time ( Figure S4, Supporting Information), verifying our viewpoint on ATPinduced hydrophobic change. In addition, noting that both the initial and final sacs are stable over two weeks without fission or fusion, suggesting that this expansion is indeed attributed to the membrane extension of single microcapsule ( Figure S5, Supporting Information).…”

Section: Polymer Design and Vesicular Self-assemblysupporting

confidence: 85%

Periodically Self‐Pulsating Microcapsule as Programmed Microseparator via ATP‐Regulated Energy Dissipation

et al. 2018

View full text Add to dashboard Cite

systems have been known very early; however, in vitro mimicking such "living" systems with adaptive features using "nonliving" molecular building blocks is still a fundamental challenge in chemistry. [5,6] To date, chemists have established a plethora of synthetic self-assembly entities, [7] some of which can undergo reversible shape changes or phase transitions under external stimuli, [8][9][10] but they are not yet comparable to their natural counterparts. The main reason is that synthetic self-assembly systems operate in thermodynamic equilibrium, resulting in timeindependent steady-state structures. In contrast, natural self-assembly requires a continuous supply of energy to keep the assemblies away from equilibrium, resulting in time-dependent dynamic structures. [11] If the energy dissipates, the system will fall apart and revert to ground state. This process is referred to as dissipative self-assembly, [12] which offers impetus for biological motions such as cell deformation and growth (in situ motion without distance change), and cell migration and division (motion with distance change). Hence, implanting this energy-driven assembly in man-made assemblies may create life-like systems, possessing structural adaption and behavioral evolution over time and space. However, such artificial systems formed by dissipative pathway are quite limited thus far. The few reports focused on biocatalytic active gels, [13][14][15] switchable films, [16] and molecule-level reaction networks. [17] Nanoscale nonequilibrium assemblies have received little attention. An insuperable obstacle is how to harmonize sophisticated self-assembly process with continuous energy influx in a time-ordered manner.Herein, we report a "living" giant vesicle system that can perform autonomous and periodic pulsating motion via energy dissipation. The energy source of this pulsation derives from the cellular biochemical fuel, ATP. To achieve the energy-regulated self-assembly, our design concept is based on transient ligandreceptor supramolecular interactions between the ATP fuels and the vesicular membrane components. The system is necessary to satisfy the following conditions: (i) a self-assembling vesicle with specific ATP receptor units can toggle between expansile and contractile state by the association and dissociation of ATP; (ii) the temporary formation of ATP-polymer ligand-receptor complexes can cause the system energy gain Living systems can experience time-dependent dynamic self-assembly for periodic, adaptive behavior via energy dissipation pathway. Creating in vitro mimics is a daunting mission. Here a "living" giant vesicle system that can perform a periodic pulsating motion using adenosine-5'-triphosphate (ATP)-fuelled dissipative self-assembly is described. This dynamic system is built on transient supramolecular interactions between the polymer and cellular energy currency ATP. The vesicles capturing ATPs will deviate away from equilibrium, leading to an energy ascent that drives a continuous vesicular expansion, until a comp...

show abstract

Section: Polymer Design and Vesicular Self-assemblysupporting

confidence: 85%

Periodically Self‐Pulsating Microcapsule as Programmed Microseparator via ATP‐Regulated Energy Dissipation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The therapeutic effects of the drug loaded NPs would depend on the uptake of the particles by cancer cells (Hong et al., 2019 ). Coumarin 6 (C-6) is a fluorescent probe, which was used to represent the particles internalized the cells (Makwana et al., 2011 ). TAB-CIS/5-FU LPHNs showed higher uptake than that of CIS/5-FU LPHNs, which revealed that the LBL NPs had excellent ability to enter cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Chemo-immune synergetic therapy of esophageal carcinoma: trastuzumab modified, cisplatin and fluorouracil co-delivered lipid–polymer hybrid nanoparticles

Wang²,

Hong

2020

Drug Delivery

View full text Add to dashboard Cite

Esophageal cancer is the sixth most common cause of cancer-related death worldwide. Peptide modified nanoparticles have been engineered as novel strategies to improve esophageal adenocarcinoma (EAC) therapy. This study aimed to develop a trastuzumab (TAB) modified system for the delivery of cisplatin (CIS) and fluoropyrimidine (5-FU). In the present study, CIS and 5-FU co-encapsulated lipid–polymer hybrid nanoparticles (CIS/5-FU LPHNs) were prepared. TAB was conjugated to the surface of CIS/5-FU LPHNs to achieve TAB decorated CIS/5-FU LPHNs (TAB-CIS/5-FU LPHNs). After the in vitro assessment, a subcutaneous model was used for the in vivo study. The mean diameter of LPNHs was around 100 nm, with higher encapsulation efficacy (EE) of about 90%. The LPNHs was stable and able to release drugs in sustained manners. 63.9% of cell uptake was achieved by TAB-CIS/5-FU LPHNs, with the best in vivo antitumor ability. The best synergistic effect with the lowest CI value (0.68) was achieved at the ratio of 1/1, which was determined for the dosage of drugs in the LPHNs preparation. TAB-CIS/5-FU LPHNs provide a new strategy for synergistic treating of EAC with higher efficacy and reduced side effects, introducing this system as a candidate for EAC therapy.

show abstract

“…Coumarin-containing trifunctional probe 137, assembled using a cleavable linker, is useful for efficient enrichment and detection of glycoproteins (Tsai et al, 2010). Coumarin 6 has been used as a fluorescent probe to monitor protein aggregation and can distinguish between both amorphors and fibrillar aggregates (Makwana et al, 2011). An S N Ar reaction-triggered fluorescence probe is developed using a new fluorogenic compound derivatized from 7-aminocoumarin for oligonucleotides detection (Shibata et al, 2009).…”

Section: Coumarin-derived Fluorescent Chemosensors For Enzymesmentioning

confidence: 99%

Coumarin-Derived Fluorescent Chemosensors

Li¹,

Cai²,

Chen³

2012

Advances in Chemical Sensors

View full text Add to dashboard Cite

Coumarin 6 and 1,6-diphenyl-1,3,5-hexatriene (DPH) as fluorescent probes to monitor protein aggregation

Cited by 28 publications

References 46 publications

Periodically Self‐Pulsating Microcapsule as Programmed Microseparator via ATP‐Regulated Energy Dissipation

Periodically Self‐Pulsating Microcapsule as Programmed Microseparator via ATP‐Regulated Energy Dissipation

Chemo-immune synergetic therapy of esophageal carcinoma: trastuzumab modified, cisplatin and fluorouracil co-delivered lipid–polymer hybrid nanoparticles

Coumarin-Derived Fluorescent Chemosensors

Contact Info

Product

Resources

About