Cost-effective bacteria-based bionematicide formula to control the root-knot nematode Meloidogyne spp. on tomato plants

Asyiah, Iis Nur; Prihatin, Jekti; Hastuti, Ahda Dwi; Winarso, Sugeng; Widjayanthi, Lenny; Nugroho, Dwi; Firmansyah, Kurniawan; Pradana, Ankardiansyah Pandu

doi:10.13057/biodiv/d220630

Cited by 8 publications

(6 citation statements)

References 58 publications

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“…The plant growth in this study is associated with decreased nematode infection in the roots. The lower the severity of nematode infection in the roots, the more they can absorb nutrients and water (Asyiah et al, 2021). Root-knot nematode infection often damages root tissue and interferes with root function (Elling, 2013).…”

Section: Discussionmentioning

confidence: 99%

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Biocontrol of root-knot nematode Meloidogyne incognita in arabica coffee seedling by using fortified bacterial consortium

Pradana

Hoesain

Asyiah

et al. 2023

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The damage caused by the southern root-knot nematode (Meloidogyne incognita) in coffee plants has been reported in various countries, including Indonesia. The measures to control the nematodes on coffee seedlings and fields depend on synthetic nematicides. Addressing this issue requires not only a more environmentally friendly and cheaper technology but also equal effectiveness comparable to synthetic nematicides. This study aimed to test the effectiveness of fortified bacterial consortium (FBC) involving a combination of liquid organic fertilizer (LOF), botanical pesticide, and a bacterial consortium to control M. incognita infection in Coffea arabica seedlings. The treatments in this study consisted of control, synthetic nematicide with the active ingredient fluopyram, and various FBC concentrations ranging from 20%, 40%, 60%, 80%, and 100%. The results demonstrated that the application of 60% to 100% FBC increased plant growth. The most effective and efficient treatment for increasing plant growth was the application of 60% FBC. When compared with control plants, 60% FBC treatment resulted in 6.8% longer root, 9.5% higher plant, 5.3% heavier plant fresh weight, and 4.8% heavier root fresh weight. We also found that the application of 60% to 100% FBC increased the amount of chlorophyll in leaves. FBC application also reduced the number of nematodes in the soil up to 60.6%, the number of galls up to 286.4%, and the severity of root damage up to 118.2%. This study indicates that the application of 60% FBC is the most effective and efficient in controlling M. incognita and stimulating the growth of C. arabica seedlings.

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Section: Discussionmentioning

confidence: 99%

“…Previous researchers have succeeded in isolating, selecting, and characterizing a consortium of bacteria to control plant-parasitic nematodes (Asyiah et al, 2020;Asyiah et al, 2021;Asyiah et al, 2018). The bacterial consortium can be fortified to increase its effectiveness in controlling M. incognita infection in C. arabica plant.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of root-knot nematode Meloidogyne incognita in arabica coffee seedling by using fortified bacterial consortium

Pradana

Hoesain

Asyiah

et al. 2023

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“…Pupuk organik cair (POC) adalah jenis pupuk yang berasal dari bahan-bahan organik dalam bentuk cair. POC dapat berisi inokulan mikroba yang mengandung biakan sel-sel aktif dari mikroba fungsinal pengikat N, pelarut P, dan pelarut K (Fasusi et al, 2021), serta mengendalikan penyakit tanaman (Asyiah et al, 2021). Penggunaan mikroorganisme fungsional dalam bidang pertanian beberapa diantaranya diaplikasikan sebagai pelapis benih, ataupun untuk mempercepat proses pengomposan.…”

Section: Pendahuluanunclassified

Peningkatan Pengetahuan Petani melalui Pelatihan Pembuatan Pupuk Organik Cair di Desa Karangrejo, Gumukmas, Kabupaten Jember.

Winarso

Anggriawan²,

Mutmainnah³

et al. 2023

warta

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The use of agrochemicals is still an option in terms of providing fast nutrition and increasing plant growth more efficiently to a certain extent. However, its continuous use results in a decrease in soil quality and soil fertility, which in turn causes the accumulation of heavy metal ions in plant tissues, and affects nutritional yields and food safety. The community service activities were carried out by the SBF (Soil Biodiversity and Fertility) team of the Jember University Soil Science study program in Karangrejo Village, Gumukmas District, Jember Regency. Field visits were conducted on farmers' fields to review, evaluate and discuss qualitative characteristics of soil properties. This service activity aims to increase understanding of sustainable soil fertility management and training in making liquid organic fertilizer based on a local bacterial consortium. The results of the activity showed that farmers' knowledge of the parameters of soil properties, especially pH, nutrients, and soil organic matter, was still minimal. The level of acidity (pH) of the soil determines the factors of production through the ease with which nutrients are absorbed by plants as well as the possibility of the presence of toxic elements that can interfere with plant growth. Knowledge of soil pH is needed in relation to nutrient management and liming. A deeper understanding of several parameters of soil properties, especially soil pH values, needs to be emphasized and followed up through mentoring activities. Mentoring activities as follow-up activities for farmer groups are carried out with the target output of liquid organic fertilizer products. Entrepreneurship training for farmer groups members also needs to be carried out to build an entrepreneurial spirit and added value from livestock products by farmers so that the farmer's household economy can sustainable.

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“…Furthermore, consortium isolates Bacillus SK07, Bacillus SK14, Bacillus KB14 and Pseudomonas diminuta Pd01 can control Meloidogyne spp. and Pratylenchus coffeae on tomato and coffee, respectively (Asyiah et al 2020;Asyiah et al 2021).…”

Section: Introductionmentioning

confidence: 99%

The effects of micronutrient-enriched media on the efficacy of Bacillus subtilis as biological control agent against Meloidogyne incognita

et al. 2023

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The root-knot nematodes Meloidogyne incognita poses a serious threat to horticultural food and plantation crops. One of the effective and efficient measures to overcome this nematode is using bacteria as a biological control agent. This study aimed to test the effectiveness of Bacillus subtilis bacteria grown on media with additional micronutrients as a biological control agent for M. incognita. The test was carried out by growing bacteria in 100 mL of nutrient broth (NB) added with FeCl2, MnCl2, and CuCl2. Each type of micronutrient was administered at concentrations of 35 ppm, 40 ppm, 45 ppm, 50 ppm, and 55 ppm. As a control, NB media was used without the addition of micronutrients. The test results showed that the best performances on bacterial growth were 55 ppm MnCl2, 45 ppm MnCl2, 40 ppm MnCl2, 40 ppm CuCl2, 50 ppm MnCl2, and 35 ppm MnCl2. These six treatments were then used in an antagonism test. A total of 150 J2 M. incognita in 4 mL of suspension was put into a petri dish with a diameter of 5 cm. After that, 1 mL of each bacterial suspension was put into a petri dish following the treatment. The mortality rate of M. incognita in each treatment was 77.5% in 55 ppm MnCl2, 74.5% in 45 ppm MnCl2, 77.25% in 40 ppm MnCl2, 73.25% in 40 ppm CuCl2, 78.75% in 50 ppm MnCl2, 79% in 35 ppm MnCl2, and 53.25% in control. The same six treatments were also used to measure the chemotaxis index of M. incognita on tomato roots. Tomato seeds of the Tantyna variety were soaked for 60 minutes in a bacterial suspension grown on micronutrient-enriched media. After seven days, the tomato seed roots were removed and placed in 5 mL 20% Pluronic F-127 Gel which had been infested with 100 J2 M. incognita. After 6 hours, the number of nematodes approaching the roots was observed. The chemotaxis index in the treatments ranged from 16% to 24%, while that in the control was 33%. This study corroborates that the addition of micronutrients MnCl2 and CuCl2 to the growth medium of B. subtilis can increase antagonistic activity against M. incognita and suppress its chemotaxis response in tomato roots soaked in the bacterial suspension.

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Cost-effective bacteria-based bionematicide formula to control the root-knot nematode Meloidogyne spp. on tomato plants

Cited by 8 publications

References 58 publications

Biocontrol of root-knot nematode Meloidogyne incognita in arabica coffee seedling by using fortified bacterial consortium

Biocontrol of root-knot nematode Meloidogyne incognita in arabica coffee seedling by using fortified bacterial consortium

Peningkatan Pengetahuan Petani melalui Pelatihan Pembuatan Pupuk Organik Cair di Desa Karangrejo, Gumukmas, Kabupaten Jember.

The effects of micronutrient-enriched media on the efficacy of Bacillus subtilis as biological control agent against Meloidogyne incognita

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