Correlation between polymorphisms inADSLandGARS-AIRS-GARTgenes with inosine 5′-monophosphate (IMP) contents in Beijing-you chickens

Abstract

1. The association of single nucleotide polymorphisms identified within the ADSL (adenylosuccinate lyase deficiency) gene and GARS-AIRS-GART (glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase) gene with the content of inosine 5'-monophosphate (IMP) was studied in a population of male Beijing-you (BJY) chickens slaughtered at 90 d of age. 2. A single nucleotide polymorphism in exon 2 of the ADSL gene had an effect on IMP content. Chickens inheriting the positive allele at ADSL, both homozygous and heterozygous genotypes, had a higher content of IMP in breast muscle than did individuals without it. 3. Similar results were obtained for the GARS-AIRS-GART gene. The marker at the 5' untranslated region (UTR) of the GARS-AIRS-GART gene was also significant for the IMP value. Chickens inheriting the genotypes with the positive allele at this locus had a much higher content of IMP than did those homozygous for the unfavourable one. 4. Interactions between ADSL and GARS-AIRS-GART were detected for such traits as body weight and muscle yields in the tested population. The two loci acted in an additive fashion. Because IMP is one of the most important flavour components in meat, markers developed at these two genes, as well as the combination genotypes, could be used as potential molecular markers for improving chicken quality.

Association between ADSL, GARS-AIRS-GART, DGAT1, and DECR1 expression levels and pork meat quality traits

“…Recent studies have reported that ADSL and GARS-AIRS-GART affect IMP content in chickens (Shu et al, 2009;Ye et al, 2010). In this study, ADSL and GARS-AIRS-GART had high expression levels in LL and low expression levels in heart and liver.…”

“…Single nucleotide polymorphisms in ADSL, a gene that codes for adenylosuccinate lyase, and in GARS-AIRS-GART, a gene that codes for glycinamide ribonucleotide-aminoimidazole ribonucleotide synthetaseglycinamide ribonucleotide transformylase, affect muscle inosine monophosphate (IMP) content in chickens (Shu et al, 2007(Shu et al, , 2009Ye et al, 2010). The gene DGAT1, which affects IMP content by increasing triglyceride levels in pig muscle (Nonneman and Rohrer, 2002), is associated with improved meat quality in Landrace swine.…”

ADSL, AMPD1, and ATIC Expression Levels in Muscle and Their Correlations with Muscle Inosine Monophosphate Content in Dapulian and Hybridized Pig Species

“…Muscle IMP content had a similar trend to that of mRNA levels of ADSL, AMPD1, and ATIC. Muscle IMP content is affected by the expression of ATIC or AMPD1 in swine [4] [18] [19] and by the expression of ADSL, AMPD1, and ATIC in chicken [5] [20] [34] reported that the expression of ADSL in Danish landrace was lower than that in Wei pig and higher than that in Anqing six-white pig without significant differences. Additionally, the expression of ADSL in Wei pig was higher than that in Anqing six-white pig, which was not consistent with our results.…”

“…Studies on IMP metabolism have mainly focused on the polymorphisms and mRNA levels of AMPD1, ADSL, and ATIC and their association with IMP content in chickens [7]- [16]. The study findings revealed that the expression levels of these genes are higher in muscle than in other tissues [7] [17] and that IMP content is associated with these three genes [5] [7] [10] [11] [14] [15]. The polymorphisms of ADSL, AMPD1, and ATIC in different swine species and their associations with IMP metabolism have been reported [4] [16] [18] [19] [20].…”

High-resolution melting curve analysis of the ADSL and LPL genes and their correlation with meat quality and blood parameters in chickens

“…Ye et al (2010), for the Beijing You chicken, found 1 polymorphism locus (C3484T); the breast IMP content was significantly different among genotypes. Our results confirmed that C3484T mutations cause differences in IMP and that IMP content is lower in the homologous mutant TT type, which is consistent with the results of Ye.…”