Association between ADSL, GARS-AIRS-GART, DGAT1, and DECR1 expression levels and pork meat quality traits

Abstract

ABSTRACT. In this study, meat quality traits were compared between Chinese lard-and European lean-type pigs. The association between expression of four genes (ADSL, GARS-AIRS-GART, DGAT1, and DECR1) and meat quality traits was also investigated. Meat quality traits were found to differ significantly between pig breeds. Meat color parameter values (a* and b*) and intramuscular fat content in Anqingliubai were significantly higher than those in Landrace (P < 0.01). Meat pH at 1 and 24 h following slaughter was significantly higher in Landrace than in Wei pigs, and meat inosine monophosphate (IMP) content was significantly higher in Landrace than in Wei and Anqingliubai pigs (both P < 0.01). Expression levels of ADSL, GARS-AIRS-GART, and DGAT1 were higher in longissimus lumborum muscle than in heart or liver tissues. ADSL and GARS-AIRS-GART expression levels were correlated with meat IMP content and pH levels. The results of this study will contribute to the understanding of meat quality traits in Chinese lard-and European lean-type pigs.

ADSL, AMPD1, and ATIC Expression Levels in Muscle and Their Correlations with Muscle Inosine Monophosphate Content in Dapulian and Hybridized Pig Species

“…The different feeding methods between DPL and Wei and Anqing six-white pigs might affect the muscle IMP content and mRNA levels of ADSL resulting from. The muscle mRNA levels of AMPD1 in chickens and of ADSL in ducks are positively correlated with IMP content [33] [34], while the muscle mRNA levels of ADSL in swine are not highly correlated with IMP content [20] [34]. Our results showed that muscle IMP content was positively correlated with Open Journal of Animal Sciences the mRNA levels of ADSL, AMPD1, and ATIC.…”

“…The association between muscle IMP content and point mutations in ATIC or AMPD1 in swine [4] [18] [19] and the association between muscle IMP content and point mutations in ADSL in duck have been investigated, which can be used as a molecular marker for breeding strategies [33]. However, limited studies have focused on the relationship between IMP content and IMP-metabolic genes in chicken, duck, and swine or on the relationship between IMP content and AMPD1 in chicken, ADSL in duck, or ADSL in swine [16] [20] [33] [34]. No study has focused on the relationship between IMP content and ADSL, AMPD1, or ATIC in swine.…”

“…Muscle IMP content had a similar trend to that of mRNA levels of ADSL, AMPD1, and ATIC. Muscle IMP content is affected by the expression of ATIC or AMPD1 in swine [4] [18] [19] and by the expression of ADSL, AMPD1, and ATIC in chicken [5] [20] [34] reported that the expression of ADSL in Danish landrace was lower than that in Wei pig and higher than that in Anqing six-white pig without significant differences. Additionally, the expression of ADSL in Wei pig was higher than that in Anqing six-white pig, which was not consistent with our results.…”

“…The correlation between mRNA levels of ADSL and IMP content has been reported [20], but not the correlation between mRNA levels of ATIC and AMPD1 and IMP content.…”

“…The study findings revealed that the expression levels of these genes are higher in muscle than in other tissues [7] [17] and that IMP content is associated with these three genes [5] [7] [10] [11] [14] [15]. The polymorphisms of ADSL, AMPD1, and ATIC in different swine species and their associations with IMP metabolism have been reported [4] [16] [18] [19] [20]. In porcine muscle, researchers have studied the factors that affect IMP content, including swine species and storage temperature post-slaughter [21] [22] [23].…”