ADSL, AMPD1, and ATIC Expression Levels in Muscle and Their Correlations with Muscle Inosine Monophosphate Content in Dapulian and Hybridized Pig Species

Abstract

We investigated the relationship between muscle inosine monophosphate (IMP) content and mRNA levels of ADSL, AMPD1, and ATIC in Dapulian (DPL), Landrace × Dapulian (LDPL), and Duroc × Landrace × Dapulian (DLDPL) hybridized pigs. Methods: The total RNA in longissimus dorsi was isolated from Dapulian (DPL), Landrace × Dapulian (LDPL) and Duroc × Landrace × Dapulian (DLDPL) hybridized pigs, weighed about 95.0 kg, n = 8/species. The internal genes with highest stability (YWHAZ and RPL4) were chosen from 11 common internal genes using Quantitative real-time PCR (qPCR) and geNorm software. The mRNA levels of ADSL, AMPD1 and ATIC genes were corrected with YWHAZ and RPL4 genes. The muscular IMP content was determined by HPLC. The muscular IMP content in DPL was higher than that in LDPL and DLDPL, 25.00% (p < 0.05) and 15.56% (p > 0.05), respectively. The muscular mRNA level of ADSL gene in DPL and LDPL was higher than that in DLDPL, 24.14% and 12.07%, respectively (p < 0.05). The muscular mRNA level of ATIC gene in DPL and LDPL was higher than that in DLDPL, 66.67% and 33.33%, respectively (p < 0.05). The muscular mRNA level of AMPD1 gene in DPL and LDPL was higher than that in DLDPL, 14.49% and 33.26%, respectively. Furthermore, the IMP content was positively correlated with the mRNA level of ADSL, AMPD1 and ATIC genes, respectively (p < 0.05). The mRNA level of ADSL gene was highly related to that of AMPD1 and ATIC gene, respectively (p < 0.01), while that of AMPD1 gene was not strongly correlated with that of ATIC gene (p > 0.05). The muscular mRNA level of AMPD1, ADSL and ATIC genes and the muscular IMP content in DPL were highest, followed by those in LDPL and DLDPL. The muscular IMP content was positively correlated with the muscular mRNA level of ADSL, AMPD1 and ATIC genes, respectively.

The prevalence of ADSL (rs3788579) and CYP1A2 (rs17861162) polymorphisms in female breast cancer patients in North-West Iran

“…The metabolism of purine nucleotides has always been of clinical interest [ 26 ]. Adenylosuccinate lyase (ADSL, EC 4.3.2.2) is a bi-functional enzyme that catalyzes the de novo conversion of Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) to 5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR) and adenylosuccinate (S-AMP) to adenosine monophosphate (AMP) at the inosine branch point of the purine biosynthetic pathway, as presented below [ 27 , 28 ]: 5-aminoimidazole-4-N-succinocarboxamide ribotide to 5-aminoimidazole-4-carboxamide ribotide + fumarate. adenylosuccinate to 5'-adenylic acid + fumarate.…”

Genome-Wide Association Study on the Content of Nucleotide-Related Compounds in Korean Native Chicken Breast Meat

“…Previous studies on cattle, pigs, and chickens have used various methods to identify multiple genes related to IMP [ 16 , 17 , 36 , 37 , 38 , 39 ]. For example, AMPD1 encodes adenosine monophosphate (AMP) deaminase, which is the enzyme that generates IMP from AMP in chicken [ 36 ].…”