High-resolution melting curve analysis of the ADSL and LPL genes and their correlation with meat quality and blood parameters in chickens

Abstract

ABSTRACT. Adenylosuccinate lyase (ADSL) and lipoprotein lipase (LPL) are key enzymes in the metabolism of inosine monophosphate (IMP) and fat mass, which are important factors in meat quality evaluation. In this study, we selected 50 hens from the ISA B-line layers and Guangxi Yellow chickens, slaughtered the chickens at 120 days old, and analyzed polymorphisms in the ADSL and LPL genes using the highresolution melting curve method. Blood lipid parameters, intramuscular fat (IMF), and IMP content were higher (P < 0.05) in Guangxi Yellow chickens than in ISA B-line layers, while LPL activity was lower (P < 0.05). In exon 2 of the ADSL gene, a C3484T mutation was identified. In both breeds, the CC genotype showed the highest IMP, and IMP was the lowest in the TT genotype. In the 5ꞌ regulatory region of the LPL gene, a C293T mutation was identified. In both breeds, the CC genotype showed the lowest LPL and IMF, while IMF was the highest in the TT genotype. The percentages of individuals with the TT type in the ADSL (2015) gene, which was associated with the lowest IMP, were 16.0 and 52.0% in Guangxi chickens and ISA layers, respectively. The percentages of individuals with the CC type of the LPL gene, which was associated with the lowest LPL and IMF, were 28.0 and 44.0%, respectively. The ADSL and LPL gene mutations are correlated with differences in meat quality in different chicken breeds, and high-resolution melting curve is an effective prediction technology for these mutations.

Lower Expression of SLC27A1 Enhances Intramuscular Fat Deposition in Chicken via Down-Regulated Fatty Acid Oxidation Mediated by CPT1A

“…Total of 33 (between breeds) and seven (between ages) DEGs were associated with IMF deposition (Supplementary Table 3). There were 24 DEGs down-regulated in WC chicken, including several well-known candidate genes for lipid metabolism, such as ABCA1 (Santamarina-Fojo et al, 2001), ACACB (Xu et al, 2014), CPT1A (Akkaoui et al, 2009), LPL (Claire et al, 2013; Zhang X. D. et al, 2015), and SLC27A1 (Sebastian et al, 2009; Guitart et al, 2014), of which SLC27A1 was also more down-regulated in WRR.D180.B than in WRR.D120.B. In other words, SLC27A1 were generally lower expressed in the higher IMF muscles.…”

“…LPL (Lipoprotein lipase) has the dual functions of triglyceride hydrolase and lipoprotein uptake (Gotoda et al, 1989; Goldberg, 1996). LPL is an important candidate gene for chicken lipid metabolism, and the mRNA level and enzymatic activity of LPL was negatively correlated with IMF contents (Claire et al, 2013; Zhang X. D. et al, 2015). SLC27A1 is involved in translocation of long-chain fatty acids (LFCAs) across the plasma membrane and subsequently lipid metabolism in skeletal muscle (García-Martínez et al, 2005; Sebastian et al, 2009; Holloway et al, 2011; Guitart et al, 2014), heart (Chiu et al, 2005), 3T3-L1 cell (Lobo et al, 2007), and 293 cell (Hatch et al, 2002).…”

“…Previous studies have identified about 20 quantitative trait loci (QTL) related to chicken IMF, which are mainly located on chromosomes 1, 2, 5, 23 (Jennen et al, 2005; D'Andre et al, 2010; Ye et al, 2010; Jia et al, 2012; Liu et al, 2013; Nassar et al, 2013; Sun et al, 2013; Zhang T. et al, 2015). Otherwise, a large number of genes including GPAT1, ACC, CD36, AGPAT1 , and DGAT2 (Jeong et al, 2012), FABP (Ye et al, 2010; Serao et al, 2011), LPL (Zhang X. D. et al, 2015), DGAT1 (Li et al, 2013) were recognized as candidate genes for IMF, but their molecular mechanisms affecting IMF are still unclear. Obviously, the mechanism underlying chicken IMF deposition is very complicated, involving a lot of genes and metabolic pathways (Figure 1).…”

Gene expression and enzyme activity of lipoprotein lipase correlate with intramuscular fat content in Guangxi san-huang and Arbor Acres chickens

“…Furthermore, previous studies also suggested that LPL in abdominal adipose tissue plays a crucial role in fat accumulation in broiler chickens (Sato et al, 1999). Using high-resolution melting curves, Zhang et al (2015) showed that ADSL (adenylosuccinate lyase) and LPL gene mutations were correlated with meat quality differences in GXSH chickens and ISA B-line layers. However, knowledge of the developmental expression patterns of LPL mRNA, LPL enzyme activity, and the relationship of each with IMF contents in GXSH chicken is limited.…”

Transcriptomic Analysis Identifies Candidate Genes Related to Intramuscular Fat Deposition and Fatty Acid Composition in the Breast Muscle of Squabs (Columba)

“…Up till now, breed-related differences in the capacity of lipid accumulation have been documented in pig ( Wood et al 2004a ; Tyra et al 2013 ), cattle ( Gotoh et al 2009 ), sheep ( Qiao et al 2008 ), and chicken ( Wang et al 2016 ). A number of single nucleotide polymorphisms (SNPs) significantly associated with the IMF trait of meat have been detected in functional genes involved in the lipid metabolism, which include the FABP (fatty acid-binding protein) (pig, Cho et al 2011 ; beef cattle, Avilés et al 2013 ), LPL (lipoprotein lipase) (chicken, Zhang et al 2015 ), and DAGT1 (diacylglycerol acyltransferase) (cattle, Wu et al 2012 ). Meanwhile, effects of genetic variants on FA composition were also demonstrated ( Taniguchi et al 2004 ; Wood et al 2008 ).…”