Lower Expression of SLC27A1 Enhances Intramuscular Fat Deposition in Chicken via Down-Regulated Fatty Acid Oxidation Mediated by CPT1A

Qiu, Feng-Fang; Xie, Liang; Ma, Jinge; Luo, Wen; Zhang, Li; Chao, Zhe; Chen, Shaohao; Nie, Qinghua; Lin, Zhemin; Zhang, Xiquan

doi:10.3389/fphys.2017.00449

Cited by 56 publications

(61 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SLC27A, also known as fatty acid transport protein (FATP)4, reflects another liver-dominant, evolutionarily conserved PPARα target that takes central place in the cellular uptake and metabolism of long-chain and very long-chain fatty acids[ 56 , 57 ]. Lowered SLC27A level facilitates fat deposition via the reduction of CPT1A-mediated fatty acid oxidation[ 58 ]. A similar phenomenon has been verified in FATP4-deficient animals with aggravated liver fatty degeneration under high-fat diet[ 59 ].…”

Section: Discussionmentioning

confidence: 99%

circRNA_0046366 inhibits hepatocellular steatosis by normalization of PPAR signaling

Guo¹,

Sun²,

Chen³

et al. 2018

WJG

View full text Add to dashboard Cite

AIMTo investigate micro (mi)R-34a-antagonizing circular (circ)RNA that underlies hepatocellular steatosis.METHODSThe effect of circRNA on miR-34a was recognized by the miRNA response element (MRE), and validated by the dual-luciferase reporter assay. Its association with hepatocellular steatosis was investigated in HepG2-based hepatocellular steatosis induced by free fatty acids (FFAs; 2:1 oleate:palmitate) stimulation. After normalization of the steatosis-related circRNA by expression vector, analysis of miR-34a activity, peroxisome proliferator-activated receptor (PPAR)α level, and expression of downstream genes were carried out so as to reveal its impact on the miR-34a/PPARα regulatory system. Both triglyceride (TG) assessment and cytopathological manifestations uncovered the role of circRNA in miR-34a-dependent hepatosteatogenesis.RESULTSBioinformatic and functional analysis verified circRNA_0046366 to antagonize the activity of miR-34a via MRE-based complementation. In contrast to its lowered level during FFA-induced hepatocellular steatosis, circRNA_0046366 up-regulation abolished the miR-34a-dependent inhibition of PPARα that played a critical role in metabolic signaling pathways. PPARα restoration exerted transcriptional improvement to multiple genes responsible for lipid metabolism. TG-specific lipolytic genes [carnitine palmitoyltransferase 1A (CPT1A) and solute-carrier family 27A (SLC27A)] among these showed significant increase in their expression levels. The circRNA_0046366-related rebalancing of lipid homeostasis led to dramatic reduction of TG content, and resulted in the ameliorated phenotype of hepatocellular steatosis.CONCLUSIONDysregulation of circRNA_0046366/miR-34a/PPARα signaling may be a novel epigenetic mechanism underlying hepatocellular steatosis. circRNA_0046366 serves as a potential target for the treatment of hepatic steatosis.

show abstract

Section: Discussionmentioning

confidence: 99%

circRNA_0046366 inhibits hepatocellular steatosis by normalization of PPAR signaling

Guo¹,

Sun²,

Chen³

et al. 2018

WJG

View full text Add to dashboard Cite

show abstract

“…IMF deposition is primarily dependent on the differentiation, maturation and proliferation of intramuscular preadipocytes (Cristancho and Lazar, 2011;Zhang et al, 2019). Previous studies have identified about several genes related to chicken IMF, including PPARG, GPAT1, ACC, CD36, AGPAT1, and DGAT2, FABP, LPL, DGAT1, and SCL27A1 (Ye et al, 2010;Serão et al, 2011;Jeong et al, 2012;Li et al, 2013;Qiu et al, 2017). The mechanism that underlies chicken IMF deposition is very complicated obviously, involving many metabolic pathways and genes.…”

Section: Introductionmentioning

confidence: 99%

The Landscape of DNA Methylation Associated With the Transcriptomic Network of Intramuscular Adipocytes Generates Insight Into Intramuscular Fat Deposition in Chicken

Zhang

Zhai

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Intramuscular fat (IMF), which regulated by genetics, nutrition and environment is an important factor that influencing meat quality. Up to now, the epigenetic regulation mechanism underlying poultry IMF deposition remains poorly understood. Here, we focused on the DNA methylation, which usually regulate genes in transcription level. To look into the essential role of DNA methylation on the IMF deposition, chicken intramuscular preadipocytes were isolated and cultured in vitro, and a model of intramuscular adipocyte differentiation was constructed. Combined the whole genome bisulfite sequencing (WGBS) and RNA-Seq technologies, we identified several methylated genes, which mainly affecting fatty acid metabolism and muscle development. Furthermore, we reported that DNA methylation regulate intramuscular adipogenesis by regulating the genes, such as collagen, type VI, alpha 1 (COL6A1) thus affecting IMF deposition. Overexpression of COL6A1 increases the lipid droplet and inhibits cell proliferation by regulating CHAD and CAMK2 in intramuscular adipocytes, while knockdown of COL6A1 shows the opposite effect. Taken together, our results reveal that DNA methylation plays an important role in poultry IMF deposition.

show abstract

“…Meat quality plays an essential role in consumer acceptance, and intramuscular fat (IMF), deposited within the muscle tissue, is a widely acknowledged indicator of meat quality [1]. Numerous studies have explored the molecular mechanisms behind differential IMF deposition using different chicken breeds with typically different IMF contents [2][3][4]; however, the different genetic backgrounds of the chickens used makes explaining the inconsistent results of these studies difficult. As they have the same genetic origin, IMF-divergent selection lines are considered a good model for studying the molecular mechanisms behind IMF deposition.…”

Section: Introductionmentioning

confidence: 99%

Effect of Divergent Selection for Intramuscular Fat Content on Muscle Lipid Metabolism in Chickens

Liu

Cui

Xing

et al. 2019

Animals

View full text Add to dashboard Cite

Simple Summary: Intramuscular fat is an important factor affecting meat quality and consumer acceptance. Appropriate increases in the intramuscular fat content contribute to the improvement of meat quality, and genetic selection is an effective method to increase the intramuscular fat content in chickens. In this study, chicken lines divergently selected for their intramuscular fat content were used to investigate the mechanisms behind differential intramuscular fat deposition. These results found in this study may contribute to the improvement of meat quality in chickens.Abstract: Intramuscular fat (IMF)-an important factor affecting meat quality-can be appropriately increased by genetic selection. Chicken lines divergently selected for IMF content were used in this study to investigate the mechanisms behind differential IMF deposition. Sixty 15th generation chickens were genotyped using the IASCHICK 55K single nucleotide polymorphism (SNP) chip. After quality control, 59 chickens and 36,893 SNPs were available for subsequent analysis. Population structure assessment indicated that the lines were genetically differentiated. Based on the top 1% paired fixation index values, three pathways were significantly (p < 0.05) enriched, and nine genes were considered candidate genes for differential IMF deposition. Differences between the lines in the expressions of representative genes involved in the above pathways were detected in 16th generation chickens. This study suggests that genetic selection for increased IMF in the pectoralis major muscle may enhance fatty acid synthesis, transport, and esterification, and reduce triglyceride hydrolysis. The peroxisome proliferator-activated receptor (PPAR) signaling pathway, glycerolipid metabolism, and fatty acid degradation pathway may have contributed to the differences in IMF deposition between the lines. These results contribute to the understanding of the genetic mechanisms behind IMF deposition, and the improvement of chicken meat quality.

show abstract

Lower Expression of SLC27A1 Enhances Intramuscular Fat Deposition in Chicken via Down-Regulated Fatty Acid Oxidation Mediated by CPT1A

Cited by 56 publications

References 90 publications

circRNA_0046366 inhibits hepatocellular steatosis by normalization of PPAR signaling

circRNA_0046366 inhibits hepatocellular steatosis by normalization of PPAR signaling

The Landscape of DNA Methylation Associated With the Transcriptomic Network of Intramuscular Adipocytes Generates Insight Into Intramuscular Fat Deposition in Chicken

Effect of Divergent Selection for Intramuscular Fat Content on Muscle Lipid Metabolism in Chickens

Contact Info

Product

Resources

About