Controlled layer-by-layer immobilization of horseradish peroxidase

Rao, Srivatsa V.; Anderson, Kimberly W.; Bachas, Leonidas G.

doi:10.1002/(sici)1097-0290(19991120)65:4<389::aid-bit3>3.0.co;2-v

Cited by 85 publications

(51 citation statements)

References 42 publications

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“…Binding proteins, such as avidin and lectin, are also useful tools for immobilizing functional molecules on solid surfaces [33,74,75,76,77,78]. Tiefenauer and coworkers prepared Fc-labeled avidin for assembling protein architectures, to which they coupled biotinylated enzymes to fabricate biosensors [79,80].…”

Section: Fc-containing Thin Filmsmentioning

confidence: 99%

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Takahashi

Anzai

2013

Materials

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This article reviews recent progress in the development of ferrocene (Fc)-modified thin films and nanoparticles in relation to their biosensor applications. Redox-active materials in enzyme biosensors commonly use Fc derivatives, which mediate electron transfer between the electrode and enzyme active site. Either voltammetric or amperometric signals originating from redox reactions of Fc are detected or modulated by the binding of analytes on the electrode. Fc-modified thin films have been prepared by a variety of protocols, including in situ polymerization, layer-by-layer (LbL) deposition, host-guest complexation and molecular recognitions. In situ polymerization provides a facile way to form Fc thin films, because the Fc polymers are directly deposited onto the electrode surface. LbL deposition, which can modulate the film thickness and Fc content, is suitable for preparing well-organized thin films. Other techniques, such as host-guest complexation and protein-based molecular recognition, are useful for preparing Fc thin films. Fc-modified Au nanoparticles have been widely used as redox-active materials to fabricate electrochemical biosensors. Fc derivatives are often attached to Au nanoparticles through a thiol-Au linkage. Nanoparticles consisting of inorganic porous materials, such as zeolites and iron oxide, and nanoparticle-based composite materials have also been used to prepare Fc-modified nanoparticles. To construct biosensors, Fc-modified nanoparticles are immobilized on the electrode surface together with enzymes.

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Section: Fc-containing Thin Filmsmentioning

confidence: 99%

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Takahashi

Anzai

2013

Materials

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“…3). A variety of enzymes have been built into LbL films for enzyme biosensors, including GOx, 75 alcohol oxidase, 76 HRP, 77 and glutamate oxidase. 78 The amperometric response of all the sensors increased with the number of enzyme layers in the LbL film, confirming that the enzymes retain their catalytic activity and that enzyme substrates penetrate the LbL film.…”

Section: ·2 Biological Affinity-based Lbl Filmsmentioning

confidence: 99%

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012

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We present an overview of the preparation and properties of layer-by-layer (LbL) deposited thin films and microcapsules in relation to their use in the development of biosensors and controlled release systems. Enzyme biosensors can be constructed by immobilizing enzymes on the surface of electrodes by LbL deposition without loss of their catalytic activity. In addition to synthetic polymers, binding proteins, such as avidin and lectin, are also used for constructing LbL films through avidin-biotin and lectin-sugar interactions. The performance characteristics of LbL film-based biosensors can be tuned by controlling the number of layers and by the choice of film components. The permeability of polyelectrolyte LbL films to ions and molecules is discussed in relation to the use of the films for eliminating interference in biosensors. The possible use of polysaccharide LbL film-coated electrodes for the construction of biosensors is highlighted, and examples of LbL film-and microcapsule-based optical sensors are described. We then focus on the use of LbL films and microcapsules as vehicles for controlled release, in particular on recent progress in the controlled release of insulin from LbL films and microcapsules. LbL films composed of insulin and polymers are sensitive to environmental pH, and release insulin in response to pH changes, suggesting that insulin LbL films can be used in the development of orally administered insulin. The construction of glucose-dependent insulin release systems using LbL insulin microcapsules functionalized with phenylboronic acid, lectin, and glucose oxidase is also examined.

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“…phenol and hydrogen peroxide [33]. The amount of produced chinonoimine was determined using spectrophotometrical measurements at = 510 nm.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Metalloporphyrin-based acetate-selective electrodes as detectors for enzymatic acetylcholine determination in flow-injection analysis system

Górski

Mroczkiewicz

Pietrzak

et al. 2009

Analytica Chimica Acta

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Controlled layer-by-layer immobilization of horseradish peroxidase

Cited by 85 publications

References 42 publications

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Recent Progress in Ferrocene-Modified Thin Films and Nanoparticles for Biosensors

Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

Metalloporphyrin-based acetate-selective electrodes as detectors for enzymatic acetylcholine determination in flow-injection analysis system

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