Controlled Internal Drug Release Uygulaması İle Senkronizasyon Yapılarak Gebe ve Gebe Kalmayan Abaza Keçilerinde Oksidatif/Nitrozatif Stres, Leptin ve Progesteron Konsantrasyonlarının Karşılaştırılması

Abstract

The aim of this study was to determine the oxidative/nitrosative stress, leptin and progesterone concentrations in pregnant and non-pregnant Abaza goats after synchronization with controlled internal drug release (CIDR) during the breeding season. For this purpose, 40 clinically healthy Abaza goats, aged 2-4 years, were intravaginally exposed to CIDR on day 0, and injected with equine chorionic gonadotropin and prostaglandin F2α on day 9 of the experiment. CIDR was removed on day 11. At the end of the experiment, they were monitored for estrus and exposed to fertile males for mating. Blood samples were collected 8 days before synchronization, then on days 0, 11 of CIDR insertion and on mating day. A pregnancy diagnosis was conducted using transrectal ultrasonography 30 day after mating. The blood serum from 40 goats (30 pregnant + 10 non-pregnant) was used for biochemical analyses. Malondialdehyde (MDA), nitric oxide (NO), total oxidant capacity (TOC), total antioxidant capacity (TAC) and endothelial NO synthase (eNOS) activities were significantly higher on mating day in pregnant goats compared to non-pregnant goats (P<0.05). The eNOS activity and TOC concentrations were significantly higher on day 11 in pregnant goats compared to non-pregnant goats (P<0.001). Serum P4 concentration increased in pregnant group on day 11 and decreased all groups on mating day than day-8, day 0 and day 11 (P<0.001). In conclusion, the administration of CIDR to Abaza goats exacerbated oxidative and nitrosative stress.

Effect of exogenous progesterone treatment on ovarian steroid hormones and oxidant and antioxidant biomarkers during peak and low breeding seasons in dromedary she-camel

“…As MDA in serum increased significantly during breeding season, SOD and GSTs were significantly higher during the non-breeding season when compared to breeding season. Increased oxidants such as MDA and NO in goats were related to increased serum P 4 concentration after administration of intravaginal P 4 -releasing devices for estrus synchronization [54,55]. Furthermore, short-term PRID treatments increase serum P 4 levels but decrease total antioxidant capacity in dairy heifers [23].…”

Enfektif Hastalıklarda Oksidan ve Antioksidan Parametrelerin Rolü: Sistematik Bir Literatür Değerlendirmesi

“…In luteal phase in the oestrus cycle and in follicular (oestrus) period, oxidation level is high. Reactive oxygen species are dissolved in cell and are used in the regulation of guanylate cyclase activity and in vital activities such as gene transcription [2,[21][22][23][24][25][26][27][28][29][30] .…”

“…MDA, hydroxy-2-nonenal (HNE), 2-propenal (acrolein), isoprostanes, oxide glutathione (GSSG), NO, total oxidant capacity (TOC), 8-hydroxy-2-deoxyguanosine (8-OHdG) are among the most studied oxidative markers clinically for this purpose. Although oxidative stress has been studied with too many diseases and syndromes, it has been reported to be effective in the development of cancer, cardiovascular disease, neural disorders, Alzheimer's disease, mild cognitive disorder, Parkinson's disease, alcohol-related liver disease, ulcerative colitis, aging and atherosclerosis, lead poisoning, liver damage due to carbon tetrachloride, aminoglycoside, reactions from drugs and toxins, such as heavy metal toxicity, chronic and degenerative diseases such as glomerulonephritis, emphysema, porphyria, bronchopulmonary dysplasia, atherosclerosis, pancreatitis, rheumatoid arthritis, aging, neurodegenerative disorders, hemolytic anemia, cardiovascular diseases, pneumonia, sepsis, mastitis, metritis, retentio secundinarum, genital tract inflammation, acidosis, tongue-playing, sepsis, mastitis, ketosis, enteritis, respiratory, joint diseases and autoimmune disorders [14,26,28,[31][32][33][34][35][36][37][38][39][40][41][42][43] .…”

Protective Effect of Caffeic Acid Phenethyl Ester on Cadmium-Induced Liver Toxicity: A Histopathological and Biochemistry Study

“…Oxidative stress occurs as a result of the disturb of the balance between the free radicals and antioxidant system in the organism. Free radicals disrupt the structures of important organic combined such as protein, DNA, carbohydrate and lipid in the cells of living organisms, leading to lipid peroxidation (El-Sokkary et al 2010; Baser et al 2020; Kuru et al 2018; Kükürt et al 2020).…”