Content of deuterium in biological fluids and organs: Influence of deuterium depleted water on D/H gradient and the process of adaptation

Джимак, С. С.; Басов, А. А.; Барышев, М. Г.

doi:10.1134/s1607672915060071

Cited by 28 publications

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“…Our data may not be related to the DDW factor until an exact mechanism of action has been described. However, the effect of deuterium on the change of biochemical parameters values is of interest to the scientific community [13][14][15][16]25,32,47,50,56,57,64,77] et al Therefore, our data will contribute to the study of mechanisms of action in this problem. The data obtained can be used in biomedical and therapeutic research, where deuterium can be considered as an adjuvant regulator of biological processes in the obesity model.…”

Section: Discussionmentioning

confidence: 99%

“…However, whether deuterium is involved in the regulation of biological properties of in-vivo models has not been fully characterized [26,27]. Differences in the D/H ratio is manifested in the form of kinetic isotope effects, characterized by a change in the biotransformation and excretion rates of the drugs [31][32][33]. Because of its characteristics, DDW has attracted much interest in the context of obesity.…”

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confidence: 99%

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Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

et al. 2019

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In this study, we present the potential application of deuterium-depleted water (DDW) for the prevention and adjuvant treatment of obesity in rats. We tested the hypothesis that DDW can alleviate diet-induced obesity (DIO) and its associated metabolic impairments. Rats fed a high-fat diet had an increased body weight index (BWI), glucose concentration, and level of certain proinflammatory cytokines; decreased levels of insulin in the serum; decreased tryptophan and serotonin in the brain, and a decreased concentration of some heavy metals in the liver. Drinking DDW at a concentration of 10 ppm deuterium/protium (D/H) ad libitum for 3 weeks restored the BWI, glucose (serum), tryptophan (brain), and serotonin (brain) levels and concentration of Zn in the liver in the DIO animals to those of the controls. The levels of proinflammatory cytokines (IL-1β, IL-6, IFNγ) and anti-inflammatory TNFα were decreased in DIO rats, while anti-inflammatory cytokine (IL-4, IL-10) levels remained at the control levels, which is indicative of a pathophysiological syndrome. In contrast, in groups of rats treated with DDW, a significant increase in anti-inflammatory (IL-4, IL-10) and proinflammatory cytokines (IFNγ) was observed. This finding indicates a reduction in systemic inflammation in obese animals treated with DDW. Similarly, the high-fat diet caused an increased level of oxidative stress products, which was accompanied by decreased activity of both superoxide dismutase and catalase, whereas the administration of DDW decreased the level of oxidative stress and enhanced antioxidant enzyme activities.Molecules 2020, 25, 23 2 of 15 the development of new, safer and more effective pharmaceutical approaches for obesity treatment is essential.Natural water is a mixture of molecules containing stable isotopes 16 O, 17 O, 18 O, 1 H, and 2 H. The ratio of deuterium atoms to protium (D/H) in fresh and marine water is 132-156 µg/g (ppm) [7,8].Since the mass of protium H is half of the mass of deuterium D, effects arising from the replacement of isotopes influence biological systems; for example, the rates of chemical reactions between substances containing these isotopes may differ by a factor of 5-10 [9-11].Numerous publications over the last 20 years have been devoted to the study of the role of deuterium in natural (150 ppm) or reduced content in the human body [12]. It has been shown that deuterium-depleted water (DDW) exhibits antidotal properties with individual and combined effects on the dosage of pharmaceutical substances and auxiliary substances [13][14][15][16]. The mechanisms of such influence are due to the structure, physicochemical properties of DDW [17] and changes in ligand-receptor interactions in biological objects of different hierarchical levels [18]. DDW increases the rate of photosynthesis and promotes the growth of plants and aquatic animals [19,20]. In Europe, the USA, Japan, China and Russia, DDW is used for prophylactic and therapeutic purposes [21,22]. Currently, DDW is using as an adjuvant in cancer treatment ...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

et al. 2019

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“…Moreover, DDW may effect transcription and protein translation by isotope exchange reactions during thermodynamic fluctuations in DNA and its hydration shell. These changes can enhance the DNA ability to interact with transcription factors, and this leads to an increase of proteins contenting in nonspecific defense systems (heat shock proteins and enzymes with antiradical activity (18)), and therefore stimulate resistance to stress influences (19), e.g. during intoxication related to hepatic-renal insufficiency.…”

Section: Discussionmentioning

confidence: 99%

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Сергеевич

Басов

Елкина

et al. 2018

Jundishapur J Nat Pharm Prod

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The objective of the current study was to evaluate the influence of deuterium-depleted water (DDW) consumption on healthy animals and animals with hepatorenal toxicity. The influence of deuterium-depleted water on hepatorenal toxicity was investigated in Wistar rats. Deuterium-depleted water consumption during 42 days caused changes in isotopic D/H composition of rats' plasma and lyophilized tissues of internal organs (liver and kidney). Moreover, deuterium-depleted water influenced plasma biochemical parameters. Concentration of creatinine and bilirubin, as well as aspartate aminotransferase and alanine aminotransferase activities were reduced in plasma of rats with hepatorenal toxicity. Deuterium-depleted water consumption during 42 days influenced the body and internal organ weights while integral index of chronic intoxication did not increase in rats with chronic intoxication. It was also revealed that deuterium-depleted water consumption did not display any toxic effects.

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“…The latter is often associated with the traditional explanation of kinetic isotope effects, which is based on the idea that increase in their intensity is proportional to the concentration of heavy isotopes. In this case, however, isotope effects are associated with a deliberate decrease in the concentration of heavy nonradioactive isotopes in comparison to their natural concentration [11] . According to data found in literature, among works describing changes in the proportion of isotopes of biogenic elements, those devoted to the effect of various deuterium concentrations (δD) on the body are most frequently found, which can be explained by more pronounced isotope differences in nuclear masses of 1 H and 2 H in comparison to corresponding indices of atomic weight ratio of stable isotopes of oxygen, carbon and nitrogen.…”

Section: Introductionmentioning

confidence: 99%

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

Басов

Anatolyevna

Alexandrovich

et al. 2019

ibj

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Background: This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. Methods: This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. Results: Modification of isotope (deuterium-protium [D/H]) composition of rats' blood and organ tissues with DDW (-705‰), introduced into rats' diet within four weeks, led to the formation of isotope D/H gradient between blood plasma and organ tissues and affected isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H 2 O 2 production during the incubation in DDW medium. This increased level of H 2 O 2 production was higher in the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰). Conclusion: The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.

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Content of deuterium in biological fluids and organs: Influence of deuterium depleted water on D/H gradient and the process of adaptation

Cited by 28 publications

References 10 publications

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

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