The objective of the current study was to evaluate the influence of deuterium-depleted water (DDW) consumption on healthy animals and animals with hepatorenal toxicity. The influence of deuterium-depleted water on hepatorenal toxicity was investigated in Wistar rats. Deuterium-depleted water consumption during 42 days caused changes in isotopic D/H composition of rats' plasma and lyophilized tissues of internal organs (liver and kidney). Moreover, deuterium-depleted water influenced plasma biochemical parameters. Concentration of creatinine and bilirubin, as well as aspartate aminotransferase and alanine aminotransferase activities were reduced in plasma of rats with hepatorenal toxicity. Deuterium-depleted water consumption during 42 days influenced the body and internal organ weights while integral index of chronic intoxication did not increase in rats with chronic intoxication. It was also revealed that deuterium-depleted water consumption did not display any toxic effects.
Background: This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. Methods: This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. Results: Modification of isotope (deuterium-protium [D/H]) composition of rats' blood and organ tissues with DDW (-705‰), introduced into rats' diet within four weeks, led to the formation of isotope D/H gradient between blood plasma and organ tissues and affected isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H 2 O 2 production during the incubation in DDW medium. This increased level of H 2 O 2 production was higher in the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰). Conclusion: The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.