Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Context: Mentha longifolia L. (Lamiaceae), a traditional medicinal herb, has been highly valued for exhibiting antimicrobial, antioxidant and antispasmodic properties. Objective: For the first time, the synergetic anti-inflammatory effects of deuterium depleted water (DDW) and M. longifolia essential oils (ML) were investigated in experimental sepsis. Materials and methods: Fifty Wistar rats were divided into 5 groups ( n = 10): negative control (laparotomy), CLP, treatment groups including the combination of DDWs (15 and 30 ppm) and ML (100 mg/kg b.w) and indomethacin. At 24 h after CLP induction, lipid peroxidation (LP), glutathione (GSH), glutathione in S -transferases (GST), ferric reducing ability of plasma (FRAP), myeloperoxidase (MPO), prostaglandin E2 (PGE2), and COX-2 expression were determined in the plasma and liver tissues. Results: Compared with the CLP group, the administration of DDWs and ML significantly ( p < 0.05) prevented the increase of LP, COX-2 and PGE2 levels and liver enzymes. Additionally, the decreased levels of FRAP and GSH induced by sepsis were remarkably ( p < 0.05) risen by the administration of DDWs and ML in comparison to the CLP group. However, no significant ( p > 0.05) differences were observed regarding GST, ALP and bilirubin levels. Our results also proved the synergistic anti-inflammatory activities of the DDWs and ML. The anti-inflammatory effects of the DDWs and ML were confirmed by histopathological studies. Discussion and conclusions: The combination of DDWs and ML exerted synergistic anti-inflammatory activity against CLP-induced sepsis possibly through modulating oxidative stress/antioxidant parameters.

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“…Moreover, DDW may affect cell cycle and cell proliferation by isotope exchange reactions (Sergeevich et al. 2018).…”

Section: Discussionmentioning

confidence: 99%

Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2

Rasooli

Fatemi

Hajihosseini

et al. 2019

Pharmaceutical Biology

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“…Введение в питьевой рацион воды со сниженным содержанием дейтерия приводит к уменьшению соотношения 2 H/ 1 H в тканях организма за счет реакций обмена изотопов водорода, которые происходят преимущественно вследствие изменения энтропии в живой системе [11]. Некоторые биологические эффекты воды со сниженным содержанием дейтерия изучены на организмах различного уровня [12]- [14]. Влияние реакций изотопного 2 H/ 1 H обмена на биохимические процессы и состояние организма остается малоизученным в связи с невысокой скоростью протекания этих реакций в физиологических условиях, что определяется феноменом постоянства изотопного состава материи в естественной среде обитания [15].…”

Section: Introductionunclassified

Mathematical Modeling of Open States in Double Stranded DNA Molecule Depending on 2H/1H Ratio

Джимак¹,

Drobotenko²,

Басов³

et al. 2019

Math.Biol.Bioinf.

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The evaluation results of the possible deuterium atoms effect on the DNA base pair opening are presented in the article. The cause of these processes is the replacement of protium with deuterium atom due to the increase of energy required to break the hydrogen bond. These processes can be studied by method of mathematical modeling, with account of open states between base pairs being the key condition of the adequacy of the mathematical model of the DNA. The experiment data show that the presence of deuterium in a chain of nucleotides can cause - depending on the value of hydrogen bond disruption energy - both increase and decrease in probability of open states occurrence. For example: hydrogen bond disruption energy of 0.358·10-22 n·m, non-zero probability of open states occurrence is observed in case of the absence of deuterium in the molecule, and with hydrogen bond disruption energy of 0.359·10-22 n·m or more such probability equals zero. Also, when one deuterium atom is present in a molecule, non-zero probability is observed even with hydrogen bond disruption energy equal to 0.368·10-22 n·m (i.e. more than 0.358·10-22 n·m). Thus participation of deuterium atoms in the formation of hydrogen bonds of double helixes of a DNA molecule can cause the changes in the time required for transfer of genetic information, which can explain the effect of even minor deviations in deuterium concentration in a medium on metabolic processes in a living system.

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“…According to data found in literature, among works describing changes in the proportion of isotopes of biogenic elements, those devoted to the effect of various deuterium concentrations (δD) on the body are most frequently found, which can be explained by more pronounced isotope differences in nuclear masses of 1 H and 2 H in comparison to corresponding indices of atomic weight ratio of stable isotopes of oxygen, carbon and nitrogen. However, despite the increasing number of studies on the impact of low δD product on living systems, these studies mainly focus on alterations in isotope D/H ratio in blood plasma [12][13][14] . These investigations are not often adequate to comparatively study δD in body tissues and fluids during the active consumption of water containing modified isotope composition with reduced deuterium content [15] .…”

Section: Introductionmentioning

confidence: 99%

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

Басов

Anatolyevna

Alexandrovich

et al. 2019

ibj

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Background: This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. Methods: This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. Results: Modification of isotope (deuterium-protium [D/H]) composition of rats' blood and organ tissues with DDW (-705‰), introduced into rats' diet within four weeks, led to the formation of isotope D/H gradient between blood plasma and organ tissues and affected isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H 2 O 2 production during the incubation in DDW medium. This increased level of H 2 O 2 production was higher in the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰). Conclusion: The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.

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Influence of Deuterium-Depleted Water on Hepatorenal Toxicity

Cited by 16 publications

References 15 publications

Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2

Synergistic effects of deuterium depleted water and Mentha longifolia L. essential oils on sepsis-induced liver injuries through regulation of cyclooxygenase-2

Mathematical Modeling of Open States in Double Stranded DNA Molecule Depending on 2H/1H Ratio

Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis

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