Contagem de células somáticas e características produtivas de vacas da raça holandesa em lactação

Pereira, Alfredo Ribeiro; Machado, Paulo Fernando; Sarriés, Gabriel Adrián

doi:10.1590/s0103-90162001000400001

Cited by 6 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is in accordance with [23], who also did not observe an effect of the somatic cell count on milk production and fat content. Reference [30] identified a relationship between increase of the SCC and milk constituents, just as [31] found an inversely proportional relationship between SCC and fat content, which was not observed in this research.…”

Section: Milk Production and Compositioncontrasting

confidence: 68%

Blood and Milk Glutamine + Glutamate and Milk Composition in Lactating Holstein Cows in Semi-Arid of Brazil

D'Paula¹,

Martinez²,

Alves³

et al. 2014

OJVM

View full text Add to dashboard Cite

Our objectives were to measure glutamine (Gln) and glutamate (Glu) concentrations in the milk and blood of lactating Holstein cows, as well as milk composition and somatic cell count (SCC) throughout different stages of lactation. Samples were collected from 21 cows in the 1 st , 2 nd , 3 rd , 5 th and 10 th months of lactation. It analyzed Gln and Glu in the blood and Gln, Glu, lactose, fat, protein and somatic cells in the milk. Also, it measured Gln and Glu in blood from these dairy cows' calves. Results were analyzed using one-way ANOVA, T-test and Tukey's test, with a 5% significance level. There was a difference in blood [Gln] in from dairy cows (P < 0.05), with lost concentration observed at 2 nd (~0.181 µmol/mL) and 5 th (~0.190 µmol/mL) months of lactation and the highest at 10 th month (~0.276 µmol/mL). Also there was a higher protein content in the 5 th and 10 th months of lactation (~3.61% each) when compared to the other months (P < 0.05). There was no difference in calves' parameters and in all others parameters from dairy cows. We concluded that the lactation period produced a significant interference in blood [Gln] but not in milk [Gln] which may indicate increase in utilization or decrease in production of this amino acids during lactation in these cows under tropical conditions.

show abstract

Section: Milk Production and Compositioncontrasting

confidence: 68%

Blood and Milk Glutamine + Glutamate and Milk Composition in Lactating Holstein Cows in Semi-Arid of Brazil

D'Paula¹,

Martinez²,

Alves³

et al. 2014

OJVM

View full text Add to dashboard Cite

show abstract

“…Mastitis is characterized by the presence of an inflammatory response in the mammary gland caused by metabolic and physiological alterations, injuries or, more frequently, environmental or contagious pathogenic microorganisms ( Oviedo-Boyso et al , 2007 ). Studies indicate that world economic losses due to mastitis can reach 35 billion dollars per year, with no actual statistics being available for Brazil, but it is believed that these numbers are significant ( Polititis et al , 1995 ; Giraudo et al , 1997 ; Pereira et al , 2001 ).…”

Section: Introductionmentioning

confidence: 99%

Expression profile of genes associated with mastitis in dairy cattle

Fonseca

Silva

Lange³

et al. 2009

Genet. Mol. Biol.

View full text Add to dashboard Cite

In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-g and TNF-a genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p < 0.05). It was also higher in BW Holstein animals with clinical mastitis (p < 0.001), but it was not significant when Gyr cows with and without mastitis were compared (0.05 < p < 0.10). Among healthy cows, BW Holstein animals tended to present a higher expression of all genes studied, with a significant difference for the IL-2 and IFN-g genes (p < 0.001). For animals with mastitis no significant difference in gene expression was observed between the two breeds. These findings suggest that animals with mastitis develop a preferentially cell-mediated immune response. Further studies including larger samples are necessary to better characterize the gene expression profile in cows with mastitis.

show abstract

“…Além das células do sistema imune, também são encontradas no leite células epiteliais relacionadas ao término da vida secretória e provenientes da descamação do tecido alveolar, presença que sugere a eliminação de células "mortas" (Boutinaud;Jammes, 2002), que variam de forma crescente do começo para o fim da lactação (Pereira et al, 2001;Coldebella et al, 2004). Neste contexto, a contagem de células somáticas no leite é um indicador da evolução e saúde da glândula mamária (Burvenich et al, 1994;Paape et al, 2002), já que, em uma glândula mamária infectada, mais de 90% das células somáticas encontradas no leite serão neutrófilos (PMN) (Boulanger et al, 2001).…”

Section: Introductionunclassified

Contagem de células somáticas e produção leiteira em cabras Saanen estressadas via aplicação de ACTH

et al. 2012

View full text Add to dashboard Cite

RESUMO -Avaliou-se neste estudo o efeito do estresse, via administração de ACTH (hormônio adrenocorticotrófico), sobre a quantidade e qualidade do leite produzido e a indicação de estresse pela alteração no nível de cortisol. Assim, 12 cabras lactantes foram distribuídas em dois grupos: seis animais receberam aplicação de 0,06 UI de ACTH/kg de PV (desafiadas) e seis receberam solução placebo (controle), todas via intravenosa. O desafio ocorreu ao longo da lactação, com aplicação do protocolo aos 30, 60, 120 e 180 dias do início da lactação, avaliando-se em cada data os níveis de cortisol 30 minutos antes, no ato da aplicação, 60, 120 e 300 minutos depois. A produção leiteira foi mensurada diariamente durante 270 dias e, a cada 20 dias, o leite foi colhido para contagem de células somáticas (CCS) pelo método direto. Os teores de cortisol mensurados em -30 e 0 minuto antes da aplicação de ACTH foram baixos e semelhantes entre os grupos. Entretanto, a partir de 60 minutos, constatou-se efeito da aplicação de ACTH, com teor de cortisol de 61,37±6,65 versus 5,47±1,21 ng/mL e de 51,17±10,21 versus 4,67±1,21 ng/mL aos 120 minutos. Aos 300 minutos, o cortisol retornou ao nível basal. Não houve diferença na produção de leite, no teor de proteína e de gordura do leite, cujos valores nos grupos ACTH e Placebo foram 1,37±0,59 e 1,38±0,63 kg de leite, respectivamente. A contagem de células somáticas não foi influenciada pelo estresse e apresentou valores situados na faixa indicada para animais sadios. Constatou-se estresse pontual em decorrência da aplicação de ACTH, porém sem alterações de natureza quantitativa e qualitativa do leite produzido, e isso indica que atividades de manejo que estressam os animais pontualmente não trazem prejuízos ao sistema produtivo.Palavras-chave: apoptose, cortisol, leite, placebo Somatic cell count and milk production of Saanen goats stressed via application of ACTHABSTRACT -The objective of this study was to analyze the stress effect, via administration of ACTH (adrenocorticotropic hormone), on quantity and quality of milk produced and also the indication of stress by alteration of cortisol level. Twelve milking goats were divided in two groups: six animals were subjected to intravenous application of 0.06 UI of ACTH/kg of LW (challenged) and six received placebo solution (control). The challenge occurred during milking with protocol application at 30, 60, 120 and 180 days from the beginning of milking; each date the levels of cortisol were evaluated 30 minutes before, at the application of ACTH, 60, 120 and 300 minutes after ACTH. The milk production was measured daily during 270 days and every twenty days the milk was collected for somatic cell count (SCC) by the direct method. The cortisol levels measured at -30 and zero minutes before ACTH applications were low and similar between the goat groups. However, after 60 minutes it was verified the effect of ACTH application, and cortisol contents of 61.37±6.65 versus 5.47±1.21 ng/mL and of 51.17±10.21 versus 4.67±1.21 ng/mL at 120 minutes. At...

show abstract

Contagem de células somáticas e características produtivas de vacas da raça holandesa em lactação

Cited by 6 publications

References 13 publications

Blood and Milk Glutamine + Glutamate and Milk Composition in Lactating Holstein Cows in Semi-Arid of Brazil

Blood and Milk Glutamine + Glutamate and Milk Composition in Lactating Holstein Cows in Semi-Arid of Brazil

Expression profile of genes associated with mastitis in dairy cattle

Contagem de células somáticas e produção leiteira em cabras Saanen estressadas via aplicação de ACTH

Contact Info

Product

Resources

About