Construction of a molecular database for soybean cultivar identification in Brazil

Oliveira, M. B.; Vieira, Elisa Serra Negra; Schuster, Ivan

doi:10.4238/vol9-2gmr706

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…Microsatellite markers are widely used for the determination of genetic diversity, paternity tests, varietal purity analysis, and genetic mapping (Schuster et al, 2004;Song et al, 2004;Garcia et al, 2007;Rodrigues et al, 2008;Oliveira et al, 2010;Kalia et al, 2011). With the advent of automatic techniques for genotyping, the use of fluorescent primers for DNA fragment analysis has become quite common (Lazaruk et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence

Ribeiro

Tanure

Maciel

et al. 2013

Pesq. agropec. bras.

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-The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic data used for varietal registration. The 13 markers allowed the distinction of all analyzed cultivars. The low cost of the UTSP method, associated with its high accuracy, makes it ideal for the characterization of soybean cultivars and for the determination of genetic purity.Index terms: Glycine max, cultivar protection, genetic diversity, genotyping method, random identity probability. Caracterização molecular de cultivares de soja por meio de marcadores microssatélites com sequência de cauda universalResumo -O objetivo deste trabalho foi padronizar um método semi-automatizado para genotipagem de soja, baseado na metodologia de iniciadores com sequências de cauda universal (PSCU), e compará-lo ao método de genotipagem convencional de eletroforese em gel de poliacrilamida. Trinta cultivares de soja foram caracterizadas genotipicamente por ambos os métodos, com o uso de 13 locos microssatélites. Para o método PSCU, o número de alelos (NA) foi de 50 (2-7 por marcador) e o conteúdo de informação polimórfica (PIC) variou de 0,40 a 0,74. Para o método convencional, o NA foi de 38 (2-5 por marcador) e o PIC variou de 0,39 a 0,67. As matrizes de dissimilaridade genética obtidas pelos dois métodos apresentaram alta correlação entre si (0,8026), e os grupos formados foram coerentes com dados fenotípicos utilizados para o registro varietal. Os 13 marcadores permitiram a distinção de todas as cultivares analisadas. O baixo custo do método PSCU, associado a sua alta acurácia, torna-o ideal para a caracterização de cultivares de soja e a determinação de pureza genética.Termos para indexação: Glycine max, proteção de cultivar, diversidade genética, método de genotipagem, probabilidade de identidade ao acaso.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence

Ribeiro

Tanure

Maciel

et al. 2013

Pesq. agropec. bras.

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“…Li and Nelson (2001) reported that the number of accessions of each region was not representative of the diversity found in each country, and the data allowed the identification of genetic patterns. Another method of identifying genotypes individually is molecular characterization (Oliveira et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Genetic divergence in a soybean (Glycine max) diversity panel based on agro-morphological traits

et al. 2016

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Owing to the narrow genetic basis of soybean (Glycine max), the incorporation of new sources of germplasm is indispensable when searching for alleles that contribute to a greater diversity of varieties. The alternative is plant introduction, which may increase genetic variability within breeding programs. Multivariate techniques are important tools to study genetic diversity and allow the precise elucidation of variability in a set of genotypes of interest. The agro-morphological traits of 93 soybean accessions from various continents were analyzed in order to assess the genetic diversity present, and to highlight important traits. The experimental design was incomplete blocks (Alpha lattice, 8 x 12) with three replicates. Nine agro-morphological traits were analyzed, and principal component analysis and cluster analysis were performed, the latter by Ward's method. The dendrogram obtained contained eight subgroups, confirming the genetic diversity among the accessions and revealing similarities between 11 national genotypes. The geographical origin of the accessions was not always related to the clusters. The traits evaluated, and the methods used, facilitated the distinction and characterization of genotypes between and within groups, and could be used in Brazilian soybean breeding programs.

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“…These values generated a minimum exclusion power of 99.99998 % (Table 4). Six SSR markers generated an overall exclusion power greater than 99.99 % in a group of 192 eucalyptus trees (Kirst et al, 2005), whereas 13 SSR markers conferred more than 99.9999 % of exclusion power to distinguish 32 soybean cultivars (Oliveira et al, 2010). Thus, this set of 20 SSR markers confers a high distinctiveness capacity and could be recommended as a molecular descriptor for maize cultivar registration purposes.…”

Section: Molecular Identity Testmentioning

confidence: 95%

Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids

Ribeiro

Pinto

Maciel

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10 −7 to 10 −14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.

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Construction of a molecular database for soybean cultivar identification in Brazil

Cited by 8 publications

References 13 publications

Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence

Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence

Genetic divergence in a soybean (Glycine max) diversity panel based on agro-morphological traits

Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids

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