Conjugated Linoleic Acid Reduces Phorbol Ester-Induced Prostaglandin F2 Production by Bovine Endometrial Cells

Rodriguez-Sallaberry, C.; Caldari‐Torres, Cristina; Greene, Elizabeth S.; Badinga, L.

doi:10.3168/jds.s0022-0302(06)72424-9

Cited by 14 publications

(12 citation statements)

References 26 publications

(24 reference statements)

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“…The PGFM production in response to an oxytocin challenge did not differ among treatments. This differs from results in vitro in which a mixture of cis-9, trans-11 CLA and trans-10, cis-12 CLA, as well as either of the individual isomers decreased PGF 2α synthesis in ewe cotyledonary endometrium (Cheng et al, 2003) and in BEND cells (Rodriguez-Sallaberry et al, 2006). It has also been reported that CLA decreased uterine prostaglandin production in pregnant rats (Harris et al, 2001), but not in gilts (Chartrand et al, 2003).…”

Section: Discussioncontrasting

confidence: 63%

Evaluation of the Mechanism of Action of Conjugated Linoleic Acid Isomers on Reproduction in Dairy Cows

Castañeda-Gutiérrez

Benefield

Veth³

et al. 2007

Journal of Dairy Science

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The objective of this study was to evaluate the mechanism of action through which conjugated linoleic acid (CLA) beneficially affects reproduction. Lactating Holstein cows (n = 45, 20 +/- 1 DIM) were assigned to 1 of 3 treatments: 70 g/d of Ca salts of tallow (control); 63 g/d of lipid-encapsulated CLA providing 7.1 g/d of cis-9, trans-11 CLA and 2.4 g/d of trans-10, cis-12 CLA (CLA 75:25); or 76 g/d of lipid-encapsulated CLA providing 7.1 g/d each of cis-9, trans-11 and trans-10, cis-12 CLA (CLA 50:50). Supplements were top-dressed for 37 d, milk production and DMI were recorded daily, and blood samples were taken 3 times per week. At 30 +/- 3 DIM, ovulation was synchronized in all cows with a modified Ovsynch protocol, and on d 15 of the cycle cows received an oxytocin injection; blood samples were obtained frequently to measure 13,14 dihydro, 15-keto PGF2alpha. On d 16 of the cycle cows received a PGF2alpha injection and ovarian follicular aspiration was performed 54 h later. Follicular fluid was analyzed for fatty acids, progesterone, and estradiol. Endometrial biopsies were taken before and again near the end of the supplementation period for fatty acid analysis. The CLA resulted in decreased milk fat content of 14.1 and 6.1% at wk 5 of treatment of CLA 50:50 and CLA 75:25, respectively. There were no differences in energy balance or plasma nonesterified fatty acids; however, plasma IGF-I was greater in cows supplemented with CLA 50:50. The CLA isomers were not detectable in endometrial tissue, but cis-9, trans-11 CLA tended to be greater in follicular fluid of supplemented cows. Response to the oxytocin challenge was not different among treatments. Progesterone during the early luteal phase and the estradiol:progesterone ratio in follicular fluid tended to be greater in cows supplemented with CLA 50:50. Overall, these results indicate that short periods of CLA supplementation do not alter uterine secretion of PGF2alpha. The mechanism through which CLA affects reproduction may involve improved ovarian follicular steroidogenesis and increased circulating concentrations of IGF-I.

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Section: Discussioncontrasting

confidence: 63%

Evaluation of the Mechanism of Action of Conjugated Linoleic Acid Isomers on Reproduction in Dairy Cows

Castañeda-Gutiérrez

Benefield

Veth³

et al. 2007

Journal of Dairy Science

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“…Phorbol esters stimulate PKC activity, activates COX-2 gene expression and PGF2α secretion via the mitogen-activated protein kinase (MAPK) pathway . The abilities of PDBu and OT to stimulate PGF2α synthesis in bovine endometrial tissue endometrial have been reported by Danet-Desnoyers et al (1994); Mann (2001); Guzeloglu et al (2004) and Rodriguez-Sallaberry et al (2006).…”

Section: Introductionmentioning

confidence: 55%

Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle

Bressan

Membrive

Goissis

et al. 2018

Braz. J. Vet. Res. Anim. Sci.

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Prostaglandin F2α (PGF2α) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E 2 ) and progesterone (P 4 ) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2α; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2α due to a local effect of endogenous E 2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P 4 in ipsilateral horn containing the corpus luteum (CL). The PGF2α stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2α content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10 -6 M PDBu or 10 -6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2α content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P < 0.01). However, the PGF2α levels did not differ between the UHs as a function of the CL position (9.46 ± 0.95 vs. 11 ± 0.95 pg/mL/mg; P > 0.05). The cellular stimulators promoted an increase in PGF2α synthesis (P < 0.02), and the effects differed among the animals (P < 0.04). The PGF2a production was higher in the explants treated with PDBu rather than OT (13.68 ± 1.16 vs. 10.01 ± 1.16 pg/mL/mg tissue, respectively; P < 0.05). In conclusion, PGF2α synthesis is modulated by the presence of the DF (local E 2 ) but not the CL (local P 4 ), and both PDBu and OT stimulated PGF2a synthesis.A prostaglandina F2α (PGF2α) determina a luteólise em bovinos. A capacidade de manipular sua síntese endógena é indispensável para a produção animal em grande escala. O estradiol (E 2 ) e a progesterona (P 4 ) modulam diversas vias moleculares das células endometriais, incluindo a síntese de PGF2α; no entanto, pouco se sabe sobre seus mecanismos específicos. Este trabalho investigou a produção in vitro e a possível modulação da PGF2α endometrial devido a um efeito local do E 2 endógeno no corno uterino ipsilateral ao folículo dominante (FD) ou da P 4 no corno ipsilateral ao corpo lúteo (CL). Os estimuladores de PGF2α oxitocina (OT) e 12,23-dibutirato de forbol (PDBu) foram incubados com explantes endometriais, e o conteúdo de PGF2α foi mensurado. Para tal, vacas cíclicas foram sincronizadas, o desenvolvimento de FD e CL foi examinado por ultrassonografia, e no 17º dia do ciclo estral os explantes endometriais foram coletados e cultivados em meio ou suplementados com PDBu 10 -6 M ou 10 -6 M OT. As amostras de meio foram coletadas imediatamente após o tratamento e sessenta minutos depois. O radioimunoensaio mostrou que o conteúdo de PGF2α do ...

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“…To examine CLA and n-3 PUFA modulation of LPSinduced PGE 2 and PGF 2a production, replicate samples of subconfluent BEND cells were pretreated with the fatty acids (100 µM) for 24 h and then challenged with LPS (1 µg/mL) for an additional 24 h. Fatty acids were complexed with bovine serum albumin (BSA; 1:2 ratio) for 2 h in a 37 °C water bath prior to addition to the cultures. The concentrations of fatty acids and the duration of fatty acid treatment (24 h) were based on previous studies in our laboratory, which indicated that these concentrations of PUFA elicited maximal physiological effects at 24 h of treatment of BEND cells (5,9). Cultures were then challenged with LPS (1 µg/mL) for an additional 24 h. After LPS treatment, samples of cell-conditioned media (500 µL) were collected and stored at -80 °C for subsequent PG assays.…”

Section: Fatty Acid Treatment Of Bend Cellsmentioning

confidence: 99%

“…One hypothesis of the mechanism of the antitumorigenic activity of CLA has been that this lipid alters the fatty acid composition of cell membrane phospholipids resulting in modulation of eicosanoids. In fact, a previous study in our laboratory demonstrated that CLA was a potent suppressor of phorbol ester-induced PGE 2 and PGF 2α in cultured bovine endometrial (BEND) cells (5). The fact that pretreatment with CLA did not affect prostaglandin H synthase-2 (PGHS-2) gene expression in cultured BEND cells suggested that, in this cell type, CLA modulation of prostaglandin (PG) biosynthesis may not involve the PGHS-2 gene expression.…”

Section: Introductionmentioning

confidence: 95%

“…Because CLA and n-3 PUFA decrease inflammatory PG production in various experimental models (5,9,11), we hypothesized that these fatty acids may attenuate LPSinduced prostaglandin release by cultured BEND cells. The primary objective of this study was to examine the effects of exogenous CLA and n-3 PUFA on LPS-stimulated PGE 2 and PGF 2a production by BEND cells.…”

Section: Introductionmentioning

confidence: 99%

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CLA and EPA inhibit LPS-induced prostaglandin release from bovine endometrial cells through an NF-κB-dependent signaling mechanism

Badinga¹,

Gülay

Ealy³

2016

Turk J Vet Anim Sci

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Although their action in antagonizing arachidonic acid metabolism is a key antiinflammatory effect of conjugated linoleic acid (CLA) and omega-3 polyunsaturated fatty acids (n-3 PUFA), these molecules have other antiinflammatory effects that might occur upstream of altered eicosanoid production. We examined the effects of two CLA isomers (c9,t11 and t10,c12 isomers), n-3 PUFA (EPA and DHA), and κNF-κB inhibitor (ammonium pyrrolidine dithiocarbamate (PDTC)) on lipopolysaccharide (LPS)-induced secretion of prostaglandins (PGE 2 and PGF 2a) by bovine endometrial (BEND) cells. LPS increased PGE 2 and PGF 2a concentrations in BEND cellconditioned media in a concentration-and time-dependent manner. The c9,t11 CLA isomer and EPA decreased prostaglandin response to LPS. Addition of PDTC, alone or in combination with c9,t11 CLA or EPA, inhibited LPS-induced eicosanoid release into the culture medium. Results indicate that the c9,t11 CLA isomer and EPA inhibit the endometrial prostaglandin release in cattle and that these molecules may act through an NF-κB signaling mechanism.

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Conjugated Linoleic Acid Reduces Phorbol Ester-Induced Prostaglandin F2 Production by Bovine Endometrial Cells

Cited by 14 publications

References 26 publications

Evaluation of the Mechanism of Action of Conjugated Linoleic Acid Isomers on Reproduction in Dairy Cows

Evaluation of the Mechanism of Action of Conjugated Linoleic Acid Isomers on Reproduction in Dairy Cows

Endometrial prostaglandin F2α in vitro production and its modulation regarding dominant follicle position in cattle

CLA and EPA inhibit LPS-induced prostaglandin release from bovine endometrial cells through an NF-κB-dependent signaling mechanism

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