CLA and EPA inhibit LPS-induced prostaglandin release from bovine endometrial cells through an NF-κB-dependent signaling mechanism

Abstract

Although their action in antagonizing arachidonic acid metabolism is a key antiinflammatory effect of conjugated linoleic acid (CLA) and omega-3 polyunsaturated fatty acids (n-3 PUFA), these molecules have other antiinflammatory effects that might occur upstream of altered eicosanoid production. We examined the effects of two CLA isomers (c9,t11 and t10,c12 isomers), n-3 PUFA (EPA and DHA), and κNF-κB inhibitor (ammonium pyrrolidine dithiocarbamate (PDTC)) on lipopolysaccharide (LPS)-induced secretion of prostaglandins (PGE 2 and PGF 2a) by bovine endometrial (BEND) cells. LPS increased PGE 2 and PGF 2a concentrations in BEND cellconditioned media in a concentration-and time-dependent manner. The c9,t11 CLA isomer and EPA decreased prostaglandin response to LPS. Addition of PDTC, alone or in combination with c9,t11 CLA or EPA, inhibited LPS-induced eicosanoid release into the culture medium. Results indicate that the c9,t11 CLA isomer and EPA inhibit the endometrial prostaglandin release in cattle and that these molecules may act through an NF-κB signaling mechanism.

Conjugated linolenic acid (CLnA) isomers as new bioactive lipid compounds in ruminant-derived food products. A review

“…Investigation of CLA isomers biological features began in 1980s when Pariza et al (1979) found in bovine meat (both raw and fried) a previously unknown compound with mutagenic inhibitory activity which was confirmed to be the effect of CLA isomers (Ha et al, 1987). Thereafter, in various animal models (e.g., rats, mice and ruminants) and in humans it was proved that some CLA isomers can exert positive effect on different pathological conditions, such as atherosclerosis, diabetes, obesity and different types of cancer (Park and Pariza, 2007;Wallace et al, 2007;Badinga et al, 2016).…”

Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats

“…In the current study, acute phase proteins can be partially induced because the research period is limited to 6 h. The effects of sulfasalazine on acute phase proteins may have limited, because acute phase proteins reach maximum levels for longer periods of time ( Table 2 ). LPS effects PGE 2 levels at different doses and times [ 6 ]. Sulfasalazine inhibits cyclooxygenase (COX)-2 enzyme activity and has an anti-inflammatory effect [ 12 ].…”