Composition and antioxidant activity of propolis from three species of Scaptotrigona stingless bees

Sawaya, Alexandra Christine Helena Frankland; Calado, J; Santos, Letícia Cavalcante dos; Marcucci, María Cristina; Akatsu, Ivan Paulo; Soares, Ademilson Espencer Egea; Abdelnur, Patrícia Verardi; Cunha, Inara Pereira da; Eberlin, Marcos N.

doi:10.3896/ibra.4.01.2.03

Cited by 49 publications

(52 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…M. mondury geopropolis contained the same ions, which suggests that this bee also collects resin from S. terebinthifolius (e.g., Sawaya et al 2007Sawaya et al , 2009). This plant occurs naturally on the coastline of Brazil, in the remaining areas of the Atlantic Forest (Carvalher-Machado et al 2008), which is an area inhabited by M. mondury (Melo 2003, Souza et al 2012.…”

Section: Biological Activities Of M Mondury Geopropolis 2253mentioning

confidence: 75%

“…Previous studies of propolis produced by native bees from different Brazilian regions found ions at m/z 373 (Figures 2e and f) and 401 as diagnostic of terpenes with acid groups present in Schinus terebinthifolius resin (e.g., Sawaya et al 2006Sawaya et al , 2007Sawaya et al , 2009. M. mondury geopropolis contained the same ions, which suggests that this bee also collects resin from S. terebinthifolius (e.g., Sawaya et al 2007Sawaya et al , 2009).…”

Section: Biological Activities Of M Mondury Geopropolis 2253mentioning

confidence: 78%

See 1 more Smart Citation

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

Santos

Queiroz

Sawaya

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproliferative activities of M. mondury geopropolis, and determined its chemical profile. The antioxidant activity was determined using in vitro ABTS ·+ , · DPPH, and β-carotene/linoleic acid co-oxidation methods. The antibacterial activity was determined using a microdilution method with Pseudomonas aeruginosa, Staphylococcus aureus, and methicillin-resistant S. aureus. The antiproliferative effect was determined in tumor cell lines using the Alamar Blue assay. The chemical profile was obtained using UHPLC-MS and UHPLC-MS/MS. The butanolic fraction had the highest concentration of phenolic compounds and more potent antioxidant properties in all assays. This fraction also had bacteriostatic and bactericidal effects against all bacterial strains at low concentrations, especially S. aureus. The hexane fraction had the highest antiproliferative potential, with IC 50 values ranging from 24.2 to 46.6 µg/mL in HL-60 (human promyelocytic leukemia cell) and K562 (human chronic myelocytic leukemia cell), respectively. Preliminary chemical analysis indicates the presence of terpenes and gallic acid in the geopropolis. Our results indicate the therapeutic potential of geopropolis from M. mondury against inflammatory, oxidative, infectious, and neoplastic diseases.

show abstract

Section: Biological Activities Of M Mondury Geopropolis 2253mentioning

confidence: 75%

Section: Biological Activities Of M Mondury Geopropolis 2253mentioning

confidence: 78%

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

Santos

Queiroz

Sawaya

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…Sawaya et al (2009) studied the same Tubi-bee propolis used in this work and showed that its composition varied seasonally. The mass spectra ions found in this extract were m/z 371, 373, 401 and 471.…”

mentioning

confidence: 99%

“…On the other hand, diterpenes and prenylated compounds, which are virtually absent in propolis from temperate zones, have been reported to be the major constituents of propolis from tropical South America, along with lignans, flavonoids and other classes of compounds (Sawaya et al , 2009). …”

mentioning

confidence: 99%

Antiproliferative effects of Tubi-bee propolis in glioblastoma cell lines

et al. 2011

View full text Add to dashboard Cite

Propolis is a resin formed by a complex chemical composition of substances that bees collect from plants. Since ancient times, propolis has been used in folk medicine, due to its biological properties, that include antimicrobial, anti-inflammatory, antitumoral and immunomodulatory activities. Glioblastoma is the most common human brain tumor. Despite the improvements in GBM standard treatment, patients’ prognosis is still very poor. The aim of this work was to evaluate in vitro the Tubi-bee propolis effects on human glioblastoma (U251 and U343) and fibroblast (MRC-5) cell lines. Proliferation, clonogenic capacity and apoptosis were analyzed after treatment with 1 mg/mL and 2 mg/mL propolis concentrations for different time periods. Additionally, glioblastoma cell lines were submitted to treatment with propolis combined with temozolomide (TMZ). Data showed an antiproliferative effect of tubi-bee propolis against glioblastoma and fibroblast cell lines. Combination of propolis with TMZ had a synergic anti-proliferative effect. Moreover, propolis caused decrease in colony formation in glioblastoma cell lines. Propolis treatment had no effects on apoptosis, demonstrating a cytostatic action. Further investigations are needed to elucidate the molecular mechanism of the antitumor effect of propolis, and the study of its individual components may reveal specific molecules with antiproliferative capacity.

show abstract

“…No entanto, estudos utilizando a própolis de origem de abelhas sem ferrão têm sido descritos, demonstrando seu potencial terapêu-tico, como a atividade antimicrobiana (Ortega et al 2011, Choudhari et al 2012, antioxidante (Sawaya et al 2009) e antitumoral (Gilberto et al 2012). Além disso, entre as atividades biológicas da própolis de Apis mellifera, destacam--se a antibacteriana (Cabral et al 2009, Ortega et al 2011, antiviral (Fischer et al 2007, Cueto et al 2011, antifúngi-ca (Salomão et al 2008, Jug et al 2014) e antiparasitária (Gressler et al 2012).…”

Section: Introductionunclassified

Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV)

Peter

Picoli²,

Zani³

et al. 2017

Pesq. Vet. Bras.

View full text Add to dashboard Cite

Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV) 1 Cristina Mendes Peter Among the biological properties of propolis, the antimicrobial activity has received prominent attention. In this paper, we describe the antiviral and virucidal effect of three hydroalcoholic extracts of propolis (brown, green and jataí bees (Tetragonisca angustula), against bovine herpesvirus type-1 (BoHV-1) and bovine viral diarrhea Virus (BVDV). All hydroalcoholic extracts were obtained from ethanol extraction. The chemical composition of propolis extracts was determined by high-performance liquid chromatography coupled to mass spectrometer (UFLC-PDA-ESI-TOF/MS) to identify and quantify compounds such as caffeic acid and p-coumaric acid, chlorogenic acid, ferulic, and flavonoids such as rutin. Cell toxicity and antiviral activity of propolis extracts in monolayers of MDBK cells (Madin-Darby Bovine Kidney) were assessed by microscopic observation and quantified by the MTT assay (3-(4.5 dimethylthiazol-2yl) -2-5-diphenyl-2H-tetrazolato bromine). Propolis extract from Jataí bees proved to be less cytotoxic (1.57mg / ml) when compared to green extracts (0.78mg / ml) and brown (0.39mg/mL). Regarding antiviral activity, propolis has shown greater efficacy in both cellular treatments (post and pre-exposure) against BoHV-1 when compared to other extracts, ie, there was increased cell viability compared to cell and virus controls. Extracts from Jataí showed activity against both viruses (BoHV-1 and BVDV) infection in the pre-test, whereas brown propolis demonstrated action only against the BoHV-1 in the pre-infection method. To determine the virucidal activity, it were used different dilutions of virus, as well as different temperatures and incubation times. The green propolis at 37°C led to a greater reduction in viral titer (4.33 log ) compared to brown (3.5 log ) and jataí (3.24 log ). Jataí propolis showed the best results in both temperatures (22 o

show abstract

Composition and antioxidant activity of propolis from three species of Scaptotrigona stingless bees

Cited by 49 publications

References 6 publications

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

Antiproliferative effects of Tubi-bee propolis in glioblastoma cell lines

Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV)

Contact Info

Product

Resources

About