Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV)

Abstract

Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV) 1 Cristina Mendes Peter Among the biological properties of propolis, the antimicrobial activity has received prominent attention. In this paper, we describe the antiviral and virucidal effect of three hydroalcoholic extracts of propolis (brown, green and jataí bees (Tetragonisca angustula), against bovine herpesvirus type-1 (BoHV-1) and bovine viral diarrhea Virus (BVDV). All hydroalcoholic extracts were obtained from ethanol extraction. The chemical composition of propolis extracts was determined by high-performance liquid chromatography coupled to mass spectrometer (UFLC-PDA-ESI-TOF/MS) to identify and quantify compounds such as caffeic acid and p-coumaric acid, chlorogenic acid, ferulic, and flavonoids such as rutin. Cell toxicity and antiviral activity of propolis extracts in monolayers of MDBK cells (Madin-Darby Bovine Kidney) were assessed by microscopic observation and quantified by the MTT assay (3-(4.5 dimethylthiazol-2yl) -2-5-diphenyl-2H-tetrazolato bromine). Propolis extract from Jataí bees proved to be less cytotoxic (1.57mg / ml) when compared to green extracts (0.78mg / ml) and brown (0.39mg/mL). Regarding antiviral activity, propolis has shown greater efficacy in both cellular treatments (post and pre-exposure) against BoHV-1 when compared to other extracts, ie, there was increased cell viability compared to cell and virus controls. Extracts from Jataí showed activity against both viruses (BoHV-1 and BVDV) infection in the pre-test, whereas brown propolis demonstrated action only against the BoHV-1 in the pre-infection method. To determine the virucidal activity, it were used different dilutions of virus, as well as different temperatures and incubation times. The green propolis at 37°C led to a greater reduction in viral titer (4.33 log ) compared to brown (3.5 log ) and jataí (3.24 log ). Jataí propolis showed the best results in both temperatures (22 o

Phytochemical screening of Cordia glabrata (MART.) A.DC. extracts and its potential antioxidant, photoprotective, antimicrobial and antiviral activities

“…The virucidal activity observed can be attributed mainly to phenolic and flavonoid compounds present in the extract (Özçelik et al, 2011). Acoording to Peter et al (2017) caffeic acid and gallic acid present in propolis extracts have antiviral action, and Hochheim et al (2019) when evaluating fractions enriched in phenolic and flavonoid compounds, have identified a potent anti-HSV activity, with evident virucidal effect. The direct inactivation of the viral particles (virucidal action) presented by the C. glabrata extract may be due to the denaturation of the viral capsid proteins of the viral envelope, which may be necessary for the adsorption of the HSV-2 virus in the cellular receptors and penetration into the host cell, as well as a possible damage to the structure of the virion (Suazo et al, 2015;Álvarez et al, 2020).…”

Antiviral action of aqueous extracts of propolis fromScaptotrigona aff. postica(Hymenoptera; Apidae) against Zica, Chikungunya, and Mayaro virus

“…In the literature, studies have been observed in which the concentrations of propolis used in antiviral assays vary from 0.0003% to 5% (Amoros et al 1992), with no morphological alterations being observed in the cells (Vynograd et al 2000). However, Peter et al 2017, observed cytotoxicity of 0.097µg/mL for brown and green propolis and 0.39µg/mL for jataí propolis. In a similar study, Bankova et al (2014) evaluated the antiviral activity of a hydroalcoholic extract of Canadian propolis, where it was observed that the maximum tolerated non-cytotoxic concentration of propolis in MDBK cells was 0.032mg/mL.…”

“…Among the various animal sources of antiviral studies, propolis has gained great attention for obtaining new drugs (Bastos et al 2011; Bankova et al 2014), mainly because of its extensive and varied chemical composition (Bankova et al 2005; Bankova et al, 2014, Negri et al, 2022) that generate huge amounts of molecules of high pharmacological value. Peter et al, 2017, describe the antiviral effect of three hydroalcoholic extracts of propolis (brown, green, and jataí bees (Tetragonisca angustula), against bovine herpesvirus type-1 (BoHV-1) and bovine viral diarrhea Virus (BVDV). This propolis has shown high efficacy in both cellular treatments (post and pre-exposure) against BoHV1.…”

“…Apparently, the viral type also influences the results of antiviral activity. Peter et al (2017), observed that jataí propolis - T. angustula) obtained better antiviral results against RNA virus (BVDV) than when using DNA virus (BoHV-1). The BVDV genome consists of a single strand of RNA, surrounded by a lipid envelope, which constitutes a structural difference concerning BoHV-1, which is a DNA virus (Cueto et al 2011).…”

Extrato de Própolis no Combate ao COVID-19: um Relato de Experiência em Nível da Atenção Básica em Saúde

“…Dos vários compostos presentes na própolis, os flavonoides merecem atenção especial. Estes são compostos fenólicos que compreendem um amplo grupo de substâncias naturais (MANACH et al, 2004;PETER et al, 2017). A ingestão de flavonoides atua em vários processos fisiológicos, uma vez que estes compostos auxiliam na absorção e no mecanismo de ação de várias vitaminas, atuando nos processos de cicatrização como antioxidantes, além de apresentarem atividade antimicrobiana e imunomoduladora (GOMES et al, 2016).…”