Comparison of total phenols, flavonoids and antioxidant activity of various crude extracts of Hyoscyamus gallagheri traditionally used for the treatment of epilepsy

Abstract

Background: Most of the locally available medicinal plant is used as medicine through different ways since the ancient time. Hyoscyamus gallagheri (H. gallagheri) is one of the rare medicinal plant which is indigenous to Oman as well as Gulf countries. The main target of this study is to estimate the total phenols and flavonoids content and evaluate the antioxidant activities of the aerial parts extracts which were collected from the southern part of Oman. Methods: Initially, the powder of aerial parts of the selected plant was extracted with a polar solvent. The prepare extract was the successive fractionation by different polarities of solvents. All the extracts were used for the estimation of total phenols and flavonoids content by common Folin-Ciocalteu reagent (FCR) and calorimetric methods. In addition, the extracts were evaluated for their antioxidant activity by spectroscopic technique through α,α-diphenyl-picrylhadrazyl (DPPH) method. Results: In the present experiment, the ethyl acetate extract contains the highest amount of phenols and the lowest in hexane extract. However, the highest amount of flavonoids was obtained from butanol extracts and the lowest in water aerial extract. In addition, the highest antioxidant activity showed in the ethyl acetate aerial extract and the lowest was in the hexane aerial extract. The other aerial extracts also showed the similar antioxidant activity comparable to Gallic acid standard. Conclusion: It was also observed that there is a significant correlation between the antioxidant and total phenols. Therefore, the best activity aerial extract might be used for the treatment of human diseases in near future.

Phenol Contents and Antioxidant Activity of Sonokeling (Dalbergia latifolia Roxb) Wood

“…Figure 3 shows the plots between antioxidant activity and phenol contents, which displayed a good pattern against TPC, suggesting the dependence of D. latifolia on TPC for effectiveness (3a), while Figure 3b and 3c were resulted in random plots. This relationship is in agreement with several prior studies (Eddebbagh et al 2016;Guedes et al 2017;Amamra et al 2018;Hossain et al 2019).…”

A Comparative Study of Diospyros malabarica (Gaub) Extracts in Various Polarity-Dependent Solvents for Evaluation of Phytoconstituents and Biological Activities

“…(1) Test for Total Flavonoid Contents (TFC). Following the established colorimetric method with few modifications, the TFC was estimated in different extracts of D. malabarica using quercetin as standard described by Hossain et al (2019). 5 mg of each plant extract was added in 4 mL of methanol in volumetric flask (5 mL).…”

“…Total flavonoid contents of all tested plant extracts were determined by calibration curve of quercetin standard, and the attained results of TFC were expressed as quercetin equivalents ( μ g quercetin/mg dry sample). All the estimation for TFC investigation in the extracts was done in triplicates [ 30 ].…”

Comparative Analysis of Metabolite Profiling of Momordica charantia Leaf and the Anti-Obesity Effect through Regulating Lipid Metabolism

“…According to the results of composition analysis, we found that the types and contents of polyphenols in MCLW were significantly higher than those in MCLE. The structure of (±)-3′,4′-Methylenedioxy-5,7-dimethylepicatechin is very similar to the structure of catechin, so (±)-3′,4′-Methylenedioxy-5,7-dimethylepicatechin may function like a catechin analog, leading to the activation of AMPK, which suppresses the expression of genes encoding enzymes and transcription factors involved in adipogenesis and lipogenesis, and stimulates those involved in lipolysis [ 36 , 37 ]. This is consistent with our findings, as M. charantia extracts showed significant fatty acid metabolism regulating capacity.…”