Comparison of the genomes of 15 avian herpes‐virus isolates by restriction endonuclease analysis

Günther, Babett; Klupp, Barbara G.; Gravendyck, M.; Lohr, Jürgen; Mettenleiter, Thomas; Kaleta, E. F.

doi:10.1080/03079459708419213

Cited by 22 publications

(21 citation statements)

References 20 publications

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“…Restriction enzyme analysis of whole-virus DNA also suggests that there is more than one PsHV variant (1,18). Our data document 10 PsHV variants.…”

Section: Discussionsupporting

confidence: 49%

“…Based on serologic cross-reactivity, five PsHV subtypes have been isolated from birds with PD-like lesions (16). Also, limited comparisons of herpesviruses isolated from parrots with PD by restriction enzyme digestion of their entire genome have demonstrated a significant degree of genetic polymorphism (1,18). The observed variation in the distribution of lesions in birds with PD also suggests that there may be multiple variants of PsHV.…”

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confidence: 99%

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Detection and Heterogeneity of Herpesviruses Causing Pacheco's Disease in Parrots

Tomaszewski¹,

Wilson

Wigle

et al. 2001

J Clin Microbiol

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Pacheco's disease (PD) is a common, often fatal, disease of parrots. We cloned a virus isolate from a parrot that had characteristic lesions of PD. Three viral clones were partially sequenced, demonstrating that this virus was an alphaherpesvirus most closely related to the gallid herpesvirus 1. Five primer sets were developed from these sequences. The primer sets were used with PCR to screen tissues or tissue culture media suspected to contain viruses from 54 outbreaks of PD. The primer sets amplified DNA from all but one sample. Ten amplification patterns were detected, indicating that PD is caused by a genetically heterogeneous population of viruses. A single genetic variant (psittacid herpesvirus variant 1) amplified with all primer sets and was the most common virus variant (62.7%). A single primer set (23F) amplified DNA from all of the positive samples, suggesting that PCR could be used as a rapid postmortem assay for these viruses. PCR was found to be significantly more sensitive than tissue culture for the detection of psittacid herpesviruses.

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“…Restriction enzyme analysis of whole-virus DNA also suggests that there is more than one PsHV variant (1,18). Our data document 10 PsHV variants.…”

Section: Discussionsupporting

confidence: 49%

mentioning

confidence: 99%

Detection and Heterogeneity of Herpesviruses Causing Pacheco's Disease in Parrots

Tomaszewski¹,

Wilson

Wigle

et al. 2001

J Clin Microbiol

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“…There exist strains of different virulence and pathogenicity, but all isolates belong to one serotype. Some degree of strain differentiation by restriction endonuclease techniques is possible (Kotiw et al, 1982;Sikezdi, 1991;Gu È nther et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Comparison of commercial ELISA test kits from Australia and the USA with the serum neutralization test in cell cultures for the detection of antibodies to the infectious laryngotracheitis virus of chickens

Bauer¹,

Lohr²,

Kaleta³

1999

Avian Pathology

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The objective of this work was the evaluation under our conditions of two commercial ELISA kits for the detection of antibodies to avian infectious laryngotracheitis (ILT) virus, one from Australia (Trop-ELISA, TropBio) and one from the USA (ProFLOK-ELISA, KPL), and to compare their performance with the conventional serum neutralization test (SNT) in chick embryo liver cells. Repeatability varied considerably, particularly when using the Trop-ELISA. Therefore, if individual results are important, at least two parallel measurements per serum sample are recommended. In 89.3% of the sera tested by SNT, results of two parallel measurements did not vary by more than one 2-fold dilution step. There was good linear correlation between both ELISAs and the SNT, the correlation coef® cient for the Trop-ELISA being r 5 0.758 and for the ProFLOK-ELISA r 5 0.867. The negative/positive cut-off was rede® ned to suit our conditions. Sera with a SN titre of $ 1:4 were considered positive. Sera with # 15% absorption in the ProFLOK-ELISA were considered clearly negative. For the Trop-ELISA, extinctions of $ 0.477 were considered positive, # 0.168 clearly negative. Values in between were regarded as doubtful for young chickens and as possibly due to non-speci® c reactions in older chickens. The sensitivity and speci® city of the ELISAs relative to the SNT were 87 and 77% for the Trop-ELISA, and 95 and 60%, respectively, for the ProFLOK-ELISA. However, the results indicate that the sensitivity of the ELISA is higher than that of the SNT, because most sera showed similar deviations from SNT results with both ELISAs. Generally, both ELISAs were a suitable alternative to the SNT under our conditions, as long as only negative/positive results are required.

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“…Within the PsHVs there appears to be a significant amount of genetic heterogeneity (1,7,15,20). Restriction fragment length polymorphism (RFLP) analysis of the entire genome of 31 PsHVs of European origin produced eight major migration patterns (A through H).…”

mentioning

confidence: 99%

Molecular Phylogeny of the Psittacid Herpesviruses Causing Pacheco's Disease: Correlation of Genotype with Phenotypic Expression

Tomaszewski

Kaleta

Phalen

2003

J Virol

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Fragments of 419 bp of the UL16 open reading frame from 73 psittacid herpesviruses (PsHVs) from the United States and Europe were sequenced. All viruses caused Pacheco's disease, and serotypes of the European isolates were known. A phylogenetic tree derived from these sequences demonstrated that the PsHVs that cause Pacheco's disease comprised four major genotypes, with each genotype including between two and four variants. With the exception of two viruses, the serotypes of the virus isolates could be predicted by the genotypes. Genotypes 1 and 4 corresponded to serotype 1 isolates, genotype 2 corresponded to serotype 2 isolates, and genotype 3 corresponded to serotype 3 isolates. The single serotype 4 virus mapped to genotype 4. DNA from a virus with a unique serotype could not be amplified with primers that amplified DNA from all other PsHVs, and its classification remains unknown. Viruses representing all four genotypes were found in both the United States and Europe, and it was therefore predicted that serotypes 1, 2, and 3 were present in the United States. Serotype 4 was represented by a single European isolate that could not be genetically distinguished from serotype 1 viruses; therefore, the presence of serotype 4 in the United States could not be predicted. Viruses of genotype 4 were found to be the most commonly associated with Pacheco's disease in macaws and conures and were least likely to be isolated in chicken embryo fibroblasts in the United States. All four genotypes caused deaths in Amazon parrots, but genotype 4 was associated with Pacheco's disease only in Amazons in Europe. Genotypes 2, 3, and 4, but not 1, were found in African grey parrots. Although parrots from the Pacific distribution represent a relatively small percentage of the total number of birds with Pacheco's disease, all four genotypes were found to cause disease in these species.

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Comparison of the genomes of 15 avian herpes‐virus isolates by restriction endonuclease analysis

Cited by 22 publications

References 20 publications

Detection and Heterogeneity of Herpesviruses Causing Pacheco's Disease in Parrots

Detection and Heterogeneity of Herpesviruses Causing Pacheco's Disease in Parrots

Comparison of commercial ELISA test kits from Australia and the USA with the serum neutralization test in cell cultures for the detection of antibodies to the infectious laryngotracheitis virus of chickens

Molecular Phylogeny of the Psittacid Herpesviruses Causing Pacheco's Disease: Correlation of Genotype with Phenotypic Expression

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