Comparison of gene expression responses of zebrafish larvae to Vibrio parahaemolyticus infection by static immersion and caudal vein microinjection

Guo, Xinya; Ji, Ce; Du, Xuan; Ren, Jianfeng; Zu, Yao; Li, Weiming; Zhang, Qinghua

doi:10.1016/j.aaf.2019.08.002

Cited by 4 publications

(3 citation statements)

References 40 publications

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“…5D to F ). Our data show a significant upregulation of three zebrafish immune or inflammatory response genes, ifnphi1 , cc120a.3 , and il1b ( 81 , 82 ) ( Fig. 5D to F ).…”

Section: Discussionmentioning

confidence: 55%

Neuromasts and Olfactory Organs of Zebrafish Larvae Represent Possible Sites of SARS-CoV-2 Pseudovirus Host Cell Entry

Choi

Chan

et al. 2022

J Virol

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“…5D to F ). Our data show a significant upregulation of three zebrafish immune or inflammatory response genes, ifnphi1 , cc120a.3 , and il1b ( 81 , 82 ) ( Fig. 5D to F ).…”

Section: Discussionmentioning

confidence: 55%

Neuromasts and Olfactory Organs of Zebrafish Larvae Represent Possible Sites of SARS-CoV-2 Pseudovirus Host Cell Entry

Choi

Chan

et al. 2022

J Virol

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“…Guo et al [45] previously stated that bacterial dose of 107 CFU/mL is usually required to induce innate immunity in zebrafsh larvae via immersion. Teir suggestion was based on a compilation of studies conducted on the immune responses of zebrafsh larvae from bacterial infection [46][47][48][49].…”

Section: Discussionmentioning

confidence: 99%

Maternal Immunity Transfer of Zebrafish (Danio rerio) following Vaccination with a Live-Attenuated Vibrio harveyi Vaccine

Muhamad-Sofie

Mohamad

Azzam-Sayuti

et al. 2023

Aquaculture Research

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Vibriosis causes a major setback in the marine aquaculture industry. In controlling vibriosis, vaccination of the broodstock could help in transferring the initial immunity to the fry and perhaps giving immunity to them during the culture period. This study determines the efficacy of three live-attenuated Vibrio harveyi vaccine (LAVh) derivatives on zebrafish broodstock in providing immunological protection to their offspring against pathogenic Vibrio spp. Zebrafish were vaccinated intraperitoneally with full effective dose (ED80) and monitored for 14 weeks. On week 6, the specimens were intraperitoneally (i.p.) challenged with pathogenic 107 CFU/ml of Vibrio spp., and subsequently another batch was left to spawn. Thereafter, the antibody level of larvae was monitored for 4 weeks post-hatching. Groups of juvenile zebrafish were given booster vaccination via immersion (1 × 107 CFU/mL) with vaccines similar to their predecessor. The fish were sampled weekly for four weeks to determine their antibody profile and pro-inflammatory gene expression. Finally, at week 3, the juvenile fish were challenged with pathogenic strains of Vibrio spp. Results of antibody profiling in the adult zebrafish model indicated that freeze-dried and stale LAVh delivers a longer immunological protective duration, while the FKVh vaccination group failed to provide immunological protection against V. alginolyticus. However, the gene expression of pro-inflammatory interleukin 1β (IL-1β) in the FKVh vaccinated group was elevated longer than that in the freeze-dried LAVh vaccinated group. Nevertheless, the relative percent survival (RPS) of both vaccination groups against pathogenic Vibrio spp. displayed 100% immunity. Overall, the freeze-dried LAVh vaccine was found to confer maternal immune protection for its offspring, provide a long duration of immunological protection and cross-protection coverage against pathogenic Vibrio spp., and have a longer shelf life. It is proposed to be commercially available for farmers to protect against Vibrio spp. infection.

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“…Wound infection with V. parahaemolyticus can also lead to septicemia, which can be fatal [29][30][31]. Marine animals such as bony fish and grouper, mud crabs, and shrimp infected with V. parahaemolyticus often exhibit marked histopathological changes and increased mortality, thus resulting in huge economic losses to the aquaculture industry [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Nanolayered Double Hydroxide Inhibits the Pathogenicity of Vibrio parahaemolyticus

Wang¹,

Ma²,

Hu³

et al. 2021

Journal of Nanomaterials

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Nanolayered double hydroxide (LDH) is a type of anion layered inorganic compound whose bacteriostatic properties have recently garnered much attention. Vibrio parahaemolyticus is a marine pathogen that can lead to aquaculture diseases and substantial economic losses. Therefore, our study assessed the mechanisms by which Mg/Al-LDH prevents V. parahaemolyticus infection. Our results demonstrated that Mg/Al-LDH not only inhibited V. parahaemolyticus growth but also biofilm formation. Moreover, coupling Mg/Al-LDH with hydrogen peroxide and UV irradiation further inhibited the growth and biofilm formation of V. parahaemolyticus. Additionally, Mg/Al-LDH was found to adversely affect DNA and the gelling ability of chitosan. Furthermore, exposing V. parahaemolyticus to Mg/Al-LDH led to a 54.73% and 4.3% inhibition in the expression of the toxic genes tlh and trh, respectively. Mg/Al-LDH also improved the symptoms of V. parahaemolyticus infection in Penaeus vannamei, making this a promising candidate to prevent pathogenic bacteria infection in aquaculture.

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Comparison of gene expression responses of zebrafish larvae to Vibrio parahaemolyticus infection by static immersion and caudal vein microinjection

Cited by 4 publications

References 40 publications

Neuromasts and Olfactory Organs of Zebrafish Larvae Represent Possible Sites of SARS-CoV-2 Pseudovirus Host Cell Entry

Neuromasts and Olfactory Organs of Zebrafish Larvae Represent Possible Sites of SARS-CoV-2 Pseudovirus Host Cell Entry

Maternal Immunity Transfer of Zebrafish (Danio rerio) following Vaccination with a Live-Attenuated Vibrio harveyi Vaccine

Nanolayered Double Hydroxide Inhibits the Pathogenicity of Vibrio parahaemolyticus

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