Neuromasts and Olfactory Organs of Zebrafish Larvae Represent Possible Sites of SARS-CoV-2 Pseudovirus Host Cell Entry

Choi, Sheyne S. A.; Chan, Ho Hin; Chan, C. T.; Wang, Xiaoyang; Webb, Sarah; Leung, Ka Wing; Tsim, Karl Wah Keung; Miller, Andrew L.

doi:10.1128/jvi.01418-22

Cited by 4 publications

(1 citation statement)

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“…Working dilutions of the antibodies and phalloidin were prepared in blocking buffer followed by centrifugation at 16,873× g for 5 min to pellet any unwanted debris. Although no specificity control was run by labeling morphants or mutants with the anti-TPCN2 antibody, the antibody was previously used to immunolabel the neuromasts and olfactory organs of zebrafish larvae (Choi et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

A Role for Two-Pore Channel Type 2 (TPC2)-Mediated Regulation of Membrane Contact Sites During Zebrafish Notochord Biogenesis?

Rice,

Chan,

Kelu

et al. 2023

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We have previously shown that in the developing trunk of zebrafish embryos, two-pore channel type 2 (TPC2)-mediated Ca2+ release from endolysosomes plays a role in the formation of the skeletal slow muscle. In addition, TPC2-mediated Ca2+ signaling is required for axon extension and the establishment of synchronized activity in the primary motor neurons. Here, we report that TPC2 might also play a role in the development of the notochord of zebrafish embryos. For example, when tpcn2 was knocked down or out, increased numbers of small vacuoles were formed in the inner notochord cells, compared with the single large vacuole in the notochord of control embryos. This abnormal vacuolation was associated with embryos displaying attenuated body axis straightening. We also showed that TPC2 has a distinct pattern of localization in the notochord in embryos at ∼24 hpf. Finally, we conducted RNAseq to identify differentially expressed genes in tpcn2 mutants compared to wild-type controls, and found that those involved in actin filament severing, cellular component morphogenesis, Ca2+ binding, and structural constituent of cytoskeleton were downregulated in the mutants. Together, our data suggest that TPC2 activity plays a key role in notochord biogenesis in zebrafish embryos.

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Section: Methodsmentioning

confidence: 99%