Nanolayered Double Hydroxide Inhibits the Pathogenicity of Vibrio parahaemolyticus

Abstract

Nanolayered double hydroxide (LDH) is a type of anion layered inorganic compound whose bacteriostatic properties have recently garnered much attention. Vibrio parahaemolyticus is a marine pathogen that can lead to aquaculture diseases and substantial economic losses. Therefore, our study assessed the mechanisms by which Mg/Al-LDH prevents V. parahaemolyticus infection. Our results demonstrated that Mg/Al-LDH not only inhibited V. parahaemolyticus growth but also biofilm formation. Moreover, coupling Mg/Al-LDH with hydrogen peroxide and UV irradiation further inhibited the growth and biofilm formation of V. parahaemolyticus. Additionally, Mg/Al-LDH was found to adversely affect DNA and the gelling ability of chitosan. Furthermore, exposing V. parahaemolyticus to Mg/Al-LDH led to a 54.73% and 4.3% inhibition in the expression of the toxic genes tlh and trh, respectively. Mg/Al-LDH also improved the symptoms of V. parahaemolyticus infection in Penaeus vannamei, making this a promising candidate to prevent pathogenic bacteria infection in aquaculture.

Impact of Nanolayered Material and Nanohybrid Modifications on Their Potential Antibacterial Activity

“…Recently, there has been growing interest in the widespread application of func tionalized layered double hydroxide (LDH) (Figure 1) as a type of two-dimensional in organic layered material, especially in catalysis, for the sorption of pollutants, in bio sensors, for corrosion inhibition, drug delivery, photocatalytic water remediation and antimicrobial material development, and for environmental protection. In addition, the biocompatibility of LDH and its low toxicity and cost has attracted attention [7][8][9][10][11][12][13]. Due to the limited applicability of pure LDH, several crucial modifications have been intro duced using appropriate organic and inorganic modifying agents.…”