Comparison of Extraction Methods for Fatty Acid and Conjugated Linoleic Acid Quantification in Milk

Martha, Resa; Despal,; Toharmat, Toto; Rofiah, Noerhayati; Anggraeni, D.

doi:10.1088/1757-899x/546/4/042022

Cited by 8 publications

(11 citation statements)

References 11 publications

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“…Milk samples were homogenized before analysis by shaking for 5 min at room temperature. Lipid extraction, methylation process and GC condition used in this analysis were similar to Martha et al (2019). The amount of 100 µL milk sample was pipetted into a screw-cap tube.…”

Section: Fatty Acids Analysis Using Gcmentioning

confidence: 99%

“…Milk fat is one of the most complex natural fat because of its fatty acid composition (Amores, 2019). Therefore, detailed fatty acid profiles are necessary to demonstrate the benefit of milk fat to human health (Martha et al, 2019). Besides fatty acid saturation, the Atherogenicity Index (AI) (Ulbricht and Southgate, 1991) and Hypocholesterolemic/Hypercholesterolemic (H/H) ratio (Santos-Silva et al, 2002) was calculated from the fatty acid profiles that were used as an indicator to describe the benefits of milk fatty acids for human health.…”

Section: Introductionmentioning

confidence: 99%

“…The conventional method for determining fatty acid is Gas Chromatography (GC) (Amores, 2019). It included steps for lipid extraction, fractionation, methylation, separation and analysis by GC (Martha et al, 2019). However, this method is time-consuming, requires solvent, sophisticated equipment and skilled labour.…”

Section: Introductionmentioning

confidence: 99%

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Estimation of Milk Fatty Acids Health Index as Milk Value Added Determinant using FT-NIRS

Despal¹,

Anzhany²,

Permana³

et al. 2021

American Journal of Animal and Veterinary Sciences

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The current milk price system based on Total Plate Count (TPC) and Total Solids (TS) are less sensitive in determining milk quality. Milk fatty acids profiles reflected milk quality for human health. However, their determination using Gas Chromatography (GC) is impracticable to be included as a daily price decision determinant. The study aimed to find a model for milk value added based on milk fatty acids profiles that reflected milk quality for human health measured by pre-calibrated rapid Fourier Transform Near-Infrared Reflectance Spectroscopy (FT-NIRS) method. Two hundred fifty-six samples of milk were collected from 3 dairy farm areas. Samples were analyzed using a Milkotronic milk analyzer for fat, protein and lactose contents and Gas Chromatography (GC) for fatty acids. The data were inputted into the FT-NIRS spectrum for calibration. The regression model to calculate milk value-added that can be used as a bonus system was developed after classifying and weighting of Milk Fatty Acid Index (MFAI) determined based on expert judgment. The results showed that milk fatty acids profiles vary greatly. Eight parameters (CLA, C16:0, SFA, MUFA, LCFA, PUFA, C18:2 trans9, 12 and H/H) can be detected accurately using FT-NIRS and used in milk value-added calculation. Simpler equation was used Y = 16.38307 + 5.395582 CLA + 0.695062 PUFA -0.0244 C18:2, trans 9, 12 with R 2 = 0.950 and was validated insignificantly different as calculated from the 8 parameters. It is concluded that the milk processing industry can use milk fatty acids generated from FT-NIRS to add value to milk collected from smallholder farmers.

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Section: Fatty Acids Analysis Using Gcmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Estimation of Milk Fatty Acids Health Index as Milk Value Added Determinant using FT-NIRS

Despal¹,

Anzhany²,

Permana³

et al. 2021

American Journal of Animal and Veterinary Sciences

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“…Analysis of milk FAs carried out with three stages consists of fat separation, methylation, and identification and injection using gas chromatography (GC), similarly to the method used by Martha et al [9]. Milk sample separation was done by mixing 10 μl of milk sample with 2 ml of 25 ml H2SO4 solution in 1-liter methanol, then homogenized using vortex for 2 minutes.…”

Section: Milk Fatty Acid Profiles Analysis 221 Analysis Of Milk Fatty Acidmentioning

confidence: 99%

Identification of feeding pattern and their impact on milk fatty acid profiles from traditional dairy cows in Pangalengan Sub-district

Anzhany

Despal

Toharmat

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Fatty acids (FA) profiles especially conjugated linoleic acid (CLA) are commonly used to distinguish milk quality. However, its content is very sensitive and tends to change with a different type of feed. The study aims to identify the relationship between the feeding system and milk FA profile in the Pangalengan sub-district. The feeding system and milk FA profiles were studied using two-step post observatory research. Step one, milk was collected from 27 traditional-dairy farms using purposive random sampling and compare with a large-scale farm. The result shows that FA profiles vary greatly among traditional-farms especially for long-chain FA (LCFA) which was found in small concentrations. CLA content was also found higher in traditional-farms. Step two, five traditional-farms with the highest CLA milk content were observed for their feeding systems. Parameter observed including cow’s specification, feed type used, and feed intake. The best ration produced high milk CLA and production was the ration consisted of 38 % high-quality forages and 62 % commercial concentrate. Thus, it can be concluded that milk from traditional farms was better than milk from large-scale farms. The best milk CLA was produced with a combination of 38 % high-quality forages and 62 % commercial concentrate.

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“…In addition to proper sample preparation, selection of appropriate analytical tool is paramount for fatty acid analysis. Wide range of analytical tools, including GC [ 173 , 174 , 176 , 179 , 182 ], GC-MS, gas-chromatography vacuum ultraviolet spectroscopy (GC-Vacuum UV) [ 178 ], gas-chromatography coupled with flame ionization detection (GC-FID) [ 170 , 172 ], liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) [ 171 , 183 ], UHPLC-MS/MS [ 142 ], Raman spectroscopy [ 181 ], FTIR [ 188 , 189 ] and many more improved platforms have been used to perform FA analysis. Fatty acid analysis in LC-MS instrument showed some drawbacks in term of larger solvent consumption and lower selectivity [ 190 ].…”

Section: Fatty Acids and Cholesterolmentioning

confidence: 99%

Recent Techniques in Nutrient Analysis for Food Composition Database

et al. 2020

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Food composition database (FCD) provides the nutritional composition of foods. Reliable and up-to date FCD is important in many aspects of nutrition, dietetics, health, food science, biodiversity, plant breeding, food industry, trade and food regulation. FCD has been used extensively in nutrition labelling, nutritional analysis, research, regulation, national food and nutrition policy. The choice of method for the analysis of samples for FCD often depends on detection capability, along with ease of use, speed of analysis and low cost. Sample preparation is the most critical stage in analytical method development. Samples can be prepared using numerous techniques; however it should be applicable for a wide range of analytes and sample matrices. There are quite a number of significant improvements on sample preparation techniques in various food matrices for specific analytes highlighted in the literatures. Improvements on the technology used for the analysis of samples by specific instrumentation could provide an alternative to the analyst to choose for their laboratory requirement. This review provides the reader with an overview of recent techniques that can be used for sample preparation and instrumentation for food analysis which can provide wide options to the analysts in providing data to their FCD.

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Comparison of Extraction Methods for Fatty Acid and Conjugated Linoleic Acid Quantification in Milk

Cited by 8 publications

References 11 publications

Estimation of Milk Fatty Acids Health Index as Milk Value Added Determinant using FT-NIRS

Estimation of Milk Fatty Acids Health Index as Milk Value Added Determinant using FT-NIRS

Identification of feeding pattern and their impact on milk fatty acid profiles from traditional dairy cows in Pangalengan Sub-district

Recent Techniques in Nutrient Analysis for Food Composition Database

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