Geographical position and feed quality of farms in highland and lowland, particularly in the tropical region, cause the differences in milk quality. One of the factors that influenced milk quality is the fatty acid (FA) profile of milk. The objective of this study is to compare the FA profiles of milk produced by farms in highland and lowland regions. Milk sampling was done by random purposive method at two regions which have different altitudes. Milk sampling was conducted with 28 farmers whose cows were fed botanically diverse forages and with commercial mix concentrate of dairy cows. The FA profiles of the milk samples were analyzed using Gas Chromatography (GC-7820A/G4350B, Agilent Technologies). Individual FAME was identified by comparison to standard mixture Supelco 37 Component FAME Mix and standard CLA (O5507, Sigma-Aldrich). The FA profiles were statistically analyzed for normality test and t-test carried out by SPSS ver.22. There were nine significantly different FA profiles consisting of C6:0, C10:0, C12:0, C14:0, C16:0, C18:0, C18:1-trans9, C18:2-trans, C18:2-cis, and C18:2-CLA. The milk from the lowland region has higher PUFA content, especially CLA content, and also has lower SFA content. The conclusion is milk from highland and lowland regions consists of different FA profiles.

The current milk price system based on Total Plate Count (TPC) and Total Solids (TS) are less sensitive in determining milk quality. Milk fatty acids profiles reflected milk quality for human health. However, their determination using Gas Chromatography (GC) is impracticable to be included as a daily price decision determinant. The study aimed to find a model for milk value added based on milk fatty acids profiles that reflected milk quality for human health measured by pre-calibrated rapid Fourier Transform Near-Infrared Reflectance Spectroscopy (FT-NIRS) method. Two hundred fifty-six samples of milk were collected from 3 dairy farm areas. Samples were analyzed using a Milkotronic milk analyzer for fat, protein and lactose contents and Gas Chromatography (GC) for fatty acids. The data were inputted into the FT-NIRS spectrum for calibration. The regression model to calculate milk value-added that can be used as a bonus system was developed after classifying and weighting of Milk Fatty Acid Index (MFAI) determined based on expert judgment. The results showed that milk fatty acids profiles vary greatly. Eight parameters (CLA, C16:0, SFA, MUFA, LCFA, PUFA, C18:2 trans9, 12 and H/H) can be detected accurately using FT-NIRS and used in milk value-added calculation. Simpler equation was used Y = 16.38307 + 5.395582 CLA + 0.695062 PUFA -0.0244 C18:2, trans 9, 12 with R 2 = 0.950 and was validated insignificantly different as calculated from the 8 parameters. It is concluded that the milk processing industry can use milk fatty acids generated from FT-NIRS to add value to milk collected from smallholder farmers.

Hermetia illucens larvae or known as maggot contains considerable amount of oil in its body. This experiment aimed to evaluate the use of maggot oil as a feed supplement for reducing methanogenesis of rumen microbial culture in vitro. The oil was supplemented into a feed substrate that consisted of forage: concentrate mixture (3:2 w/w). Supplementation of the oil was performed at different levels, i.e., 0, 1, 2, 3, 4 and 5% from substrate dry matter. All incubation bottles were tightly closed and incubated for 24 h in a water bath that maintained at 39oC. Results revealed that maggot oil at 4 and 5% supplementation level was able to reduce methane emission by 20.7 and 26.9% (P<0.05) in comparison to control, respectively. However, its supplementation at 3% or lower did not alter the methane emission. The organic matter digestibility parameter was linearly reduced with increasing level of maggot oil supplementation (P<0.05), but volatile fatty acid concentration was not affected. In contrast to our expectation, maggot oil at 3 to 5% increased log protozoa population and ammonia concentration than those of control (P<0.05). In conclusion, maggot oil may serve as a promising supplement for mitigating ruminal methanogenesis and the effect is dose-dependent.

Fatty acids (FA) profiles especially conjugated linoleic acid (CLA) are commonly used to distinguish milk quality. However, its content is very sensitive and tends to change with a different type of feed. The study aims to identify the relationship between the feeding system and milk FA profile in the Pangalengan sub-district. The feeding system and milk FA profiles were studied using two-step post observatory research. Step one, milk was collected from 27 traditional-dairy farms using purposive random sampling and compare with a large-scale farm. The result shows that FA profiles vary greatly among traditional-farms especially for long-chain FA (LCFA) which was found in small concentrations. CLA content was also found higher in traditional-farms. Step two, five traditional-farms with the highest CLA milk content were observed for their feeding systems. Parameter observed including cow’s specification, feed type used, and feed intake. The best ration produced high milk CLA and production was the ration consisted of 38 % high-quality forages and 62 % commercial concentrate. Thus, it can be concluded that milk from traditional farms was better than milk from large-scale farms. The best milk CLA was produced with a combination of 38 % high-quality forages and 62 % commercial concentrate.

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