Comparison of acellular and cellular bioactivity of poly 3-hydroxybutyrate/hydroxyapatite nanocomposite and poly 3-hydroxybutyrate scaffolds

Saadat, A.; Behnamghader, Aliasghar; Karbasi, Saeed; Abedi, Daryoush; Soleimani, Masoud; Shafiee, Abbas

doi:10.1007/s12257-012-0744-4

Cited by 27 publications

(16 citation statements)

References 25 publications

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“…Then, the samples were soaked in deionized water overnight and wet weight of samples were recorded. The percentage of porosity of the composite scaffolds was calculated by using the following equation :

Porosity (%) = \frac{W_{w} - W_{normald}}{V_{a}}

where W w is the wet scaffold weight (g), W d is the dry scaffold weight (g) and V a is the apparent scaffold volume (mL).…”

Section: Methodsmentioning

confidence: 99%

Section: Porosity Of the Scaffoldmentioning

confidence: 99%

“…Polymer‐based composite scaffold showed great potential in bone tissue engineering. Efforts have thus been made to form porous PHB/HA and PHBV/HA composites for bone tissue repair by utilizing the osteoconductivity property of HA . For instance, particulate hydroxyapatite (HA) incorporated into poly(3‐hydroxybutyrate) (PHB) formed a bioactive and biodegradable composite for applications in hard tissue replacement and regeneration .…”

Section: Introductionmentioning

confidence: 99%

“…Efforts have thus been made to form porous PHB/HA and PHBV/HA composites for bone tissue repair by utilizing the osteoconductivity property of HA [10][11][12][13]. For instance, particulate hydroxyapatite (HA) incorporated into poly(3-hydroxybutyrate) (PHB) formed a bioactive and biodegradable composite for applications in hard tissue replacement and regeneration [11]. Jack et al [14] fabricated PHBV/HA composite scaffolds with high porosity and controlled pore architectures.…”

Section: Introductionmentioning

confidence: 99%

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A porous medium‐chain‐length poly(3‐hydroxyalkanoates)/hydroxyapatite composite as scaffold for bone tissue engineering

Ansari

Annuar

Murphy

2016

Engineering in Life Sciences

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Polyhydroxyalkanoates (PHA) are hydrophobic biopolymers with huge potential for biomedical applications due to their biocompatibility, excellent mechanical properties and biodegradability. A porous composite scaffold made of medium‐chain‐length poly(3‐hydroxyalkanoates) (mcl‐PHA) and hydroxyapatite (HA) was fabricated using particulate leaching technique and NaCl as a porogen. Different percentages of HA loading was investigated that would support the growth of osteoblast cells. Ultrasonic irradiation was applied to facilitate the dispersion of HA particles into the mcl‐PHA matrix. The different P(3HO‐co‐3HHX)/HA composites were investigated using field emission scanning electron microscopy (FESEM), X‐ray diffraction (XRD) and energy dispersive X‐ray analysis (EDXA). The scaffolds were found to be highly porous with interconnecting pore structures and the HA particles were homogeneously dispersed in the polymer matrix. The scaffolds biocompatibility and osteoconductivity were also assessed following the proliferation and differentiation of osteoblast cells on the scaffolds. From the results, it is clear that scaffolds made from P(3HO‐co‐3HHX)/HA composites are viable candidate materials for bone tissue engineering applications.

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Porosity (%) = \frac{W_{w} - W_{normald}}{V_{a}}

where W w is the wet scaffold weight (g), W d is the dry scaffold weight (g) and V a is the apparent scaffold volume (mL).…”

Section: Methodsmentioning

confidence: 99%

Section: Porosity Of the Scaffoldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A porous medium‐chain‐length poly(3‐hydroxyalkanoates)/hydroxyapatite composite as scaffold for bone tissue engineering

Ansari

Annuar

Murphy

2016

Engineering in Life Sciences

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“…P3HB-nHA composite prepared by solvent casting method showed pronounced cell proliferation and differentiation and higher ALP activity than the polymer alone [70], [71].…”

Section: Bacterial Derived Polymer Compositesmentioning

confidence: 94%

Design of biocomposite materials for bone tissue regeneration

Basha

Kumar

Doble

2015

Materials Science and Engineering: C

243

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Synthetic strategy for the production of electrically polarized polyvinylidene fluoride‐trifluoroethylene—co‐polymer osseo‐functionalized with hydroxyapatite scaffold

Swain,

Lenka,

Rautray

2024

J Biomedical Materials Res

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The physiological mechanism of bone tissue regeneration is intricately organized and involves several cell types, intracellular, and extracellular molecular signaling networks. To overcome the drawbacks of autografts and allografts, a number of synthetically produced scaffolds have been manufactured by integrating ceramics, polymers, and their hybrid‐composites. Considering the fact that natural bone is composed primarily of collagen and hydroxyapatite, ceramic‐polymer composite materials seem to be the most viable alternative to bone implants. Here, in this experimental study, copolymer PVDF‐TrFE has been amalgamated with HA ceramics to produce composite scaffolds as bone implants. In order to fabricate PVDF‐TrFE‐HA (polyvinylidene fluoride‐trifluoroethylene—hydroxyapatite) composite scaffolds, solvent casting‐particulate leaching technique was devised. Two scaffold specimens were produced, with different PVDF‐TrFE and HA molar ratios (70:30 and 50:50), and then electrically polarized to observe the subsequent polarization impact on the tissue growth and the suppression of bacterial cell proliferation. Both the specimens underwent characterization to analyze their biocompatibility and bactericidal activities. The bacterial culture of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) bacteria on the composites was studied to understand the antibacterial characteristics. Moreover, MG63 cells cultured on these as‐formed composites provided information about osteogenesis. Improved osteogenesis and antibacterial efficacy were observed on both the composites. However, the composite with 70 wt% PVDF‐TrFE and 30 wt% HA showed a higher bactericidal effect as well as osteogenesis. It was found that PVDF‐TrFE‐HA‐based biomaterials have the potential for bone tissue engineering applications.

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Comparison of acellular and cellular bioactivity of poly 3-hydroxybutyrate/hydroxyapatite nanocomposite and poly 3-hydroxybutyrate scaffolds

Cited by 27 publications

References 25 publications

A porous medium‐chain‐length poly(3‐hydroxyalkanoates)/hydroxyapatite composite as scaffold for bone tissue engineering

A porous medium‐chain‐length poly(3‐hydroxyalkanoates)/hydroxyapatite composite as scaffold for bone tissue engineering

Design of biocomposite materials for bone tissue regeneration

Synthetic strategy for the production of electrically polarized polyvinylidene fluoride‐trifluoroethylene—co‐polymer osseo‐functionalized with hydroxyapatite scaffold

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