A porous medium‐chain‐length poly(3‐hydroxyalkanoates)/hydroxyapatite composite as scaffold for bone tissue engineering

Ansari, Nor Faezah; Annuar, Mohamad Suffian Mohamad; Murphy, Belinda

doi:10.1002/elsc.201600084

Cited by 28 publications

(19 citation statements)

References 34 publications

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“…They are mainly utilized as scaffolds and implants in roles including repair, replacement, or diagnostic applications. [1][2][3] Hydrogel biomaterials have found widespread use in dental, skin, drug distribution, and various surgical applications. 4 The large molecular mass of the polymer contributes to the distinctive and, often advantageous, physical properties relative to the small molecule compounds.…”

Section: Introductionmentioning

confidence: 99%

Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route

et al. 2018

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Natural biopolymers have many attractive medical applications; however, complications due to fibrosis caused a reduction in diffusion and dispersal of nutrients and waste products. Consequently, severe immunocompatibility problems and poor mechanical and degradation properties in synthetic polymers ensue. Hence, the present study investigates a novel hydrogel material synthesized from caprolactone, ethylene glycol, ethylenediamine, polyethylene glycol, ammonium persulfate, and tetramethylethylenediamine via chemo-enzymatic route. Spectroscopic analyses indicated the formation of polyurea and polyhydroxyurethane as the primary building block of the hydrogel starting material. Biocompatibility studies showed positive observation in biosafety test using direct contact cytotoxicity assay in addition to active cellular growth on the hydrogel scaffold based on fluorescence observation. The synthesized hydrogel also exhibited (self) fluorescence properties under specific wavelength excitation. Hence, synthesized hydrogel could be a potential candidate for medical imaging as well as tissue engineering applications as a tissue expander, coating material, biosensor, and drug delivery system.

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Section: Introductionmentioning

confidence: 99%

Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route

et al. 2018

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“…These characteristics of fractured PHB are related to a possible improvement of the polymer surface matrix and its use in the application of cell adhesion for 3D engineering, drug release and cell proliferation studies [ 14 , 62 ]. However, to make PHB polymers biologically active and a suitable biodegradable biomaterials, it is necessary to make them softer and more elastic.…”

Section: Resultsmentioning

confidence: 99%

“…However, to make PHB polymers biologically active and a suitable biodegradable biomaterials, it is necessary to make them softer and more elastic. In this regard, PHB composites with hydroxyapatite (HA), clay, and copolymers such as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) provide good physical and mechanical properties and increase hydrophobicity and degradation [ 62 ]. The PHB improvement from Stigeoclonium sp.…”

Section: Resultsmentioning

confidence: 99%

Characterization and Biotechnological Potential of Intracellular Polyhydroxybutyrate by Stigeoclonium sp. B23 Using Cassava Peel as Carbon Source

et al. 2021

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The possibility of utilizing lignocellulosic agro-industrial waste products such as cassava peel hydrolysate (CPH) as carbon sources for polyhydroxybutyrate (PHB) biosynthesis and characterization by Amazonian microalga Stigeoclonium sp. B23. was investigated. Cassava peel was hydrolyzed to reducing sugars to obtain increased glucose content with 2.56 ± 0.07 mmol/L. Prior to obtaining PHB, Stigeoclonium sp. B23 was grown in BG-11 for characterization and Z8 media for evaluation of PHB nanoparticles’ cytotoxicity in zebrafish embryos. As results, microalga produced the highest amount of dry weight of PHB with 12.16 ± 1.28 (%) in modified Z8 medium, and PHB nanoparticles exerted some toxicity on zebrafish embryos at concentrations of 6.25–100 µg/mL, increased mortality (<35%) and lethality indicators as lack of somite formation (<25%), non-detachment of tail, and lack of heartbeat (both <15%). Characterization of PHB by scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimeter (DSC), and thermogravimetry (TGA) analysis revealed the polymer obtained from CPH cultivation to be morphologically, thermally, physically, and biologically acceptable and promising for its use as a biomaterial and confirmed the structure of the polymer as PHB. The findings revealed that microalgal PHB from Stigeoclonium sp. B23 was a promising and biologically feasible new option with high commercial value, potential for biomaterial applications, and also suggested the use of cassava peel as an alternative renewable resource of carbon for PHB biosynthesis and the non-use of agro-industrial waste and dumping concerns.

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“…However, the in vivo application of these materials is limited by their brittle nature ( Obat et al, 2013 ). Therefore, recently mcl-PHAs have also been investigated as an alternative material for bone regeneration in non-load bearing applications ( Ansari et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Composite Materials Based on the Combination of Polyhydroxyalkanoates With Selenium and Strontium Co-substituted Hydroxyapatite for Bone Regeneration

Marcello

Maqbool

Nigmatullin

et al. 2021

Front. Bioeng. Biotechnol.

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Due to the threat posed by the rapid growth in the resistance of microbial species to antibiotics, there is an urgent need to develop novel materials for biomedical applications capable of providing antibacterial properties without the use of such drugs. Bone healing represents one of the applications with the highest risk of postoperative infections, with potential serious complications in case of bacterial contaminations. Therefore, tissue engineering approaches aiming at the regeneration of bone tissue should be based on the use of materials possessing antibacterial properties alongside with biological and functional characteristics. In this study, we investigated the combination of polyhydroxyalkanoates (PHAs) with a novel antimicrobial hydroxyapatite (HA) containing selenium and strontium. Strontium was chosen for its well-known osteoinductive properties, while selenium is an emerging element investigated for its multi-functional activity as an antimicrobial and anticancer agent. Successful incorporation of such ions in the HA structure was obtained. Antibacterial activity against Staphylococcus aureus 6538P and Escherichia coli 8739 was confirmed for co-substituted HA in the powder form. Polymer-matrix composites based on two types of PHAs, P(3HB) and P(3HO-co-3HD-co-3HDD), were prepared by the incorporation of the developed antibacterial HA. An in-depth characterization of the composite materials was conducted to evaluate the effect of the filler on the physicochemical, thermal, and mechanical properties of the films. In vitro antibacterial testing showed that the composite samples induce a high reduction of the number of S. aureus 6538P and E. coli 8739 bacterial cells cultured on the surface of the materials. The films are also capable of releasing active ions which inhibited the growth of both Gram-positive and Gram-negative bacteria.

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A porous medium‐chain‐length poly(3‐hydroxyalkanoates)/hydroxyapatite composite as scaffold for bone tissue engineering

Cited by 28 publications

References 34 publications

Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route

Structural and bone marrow stem cell biocompatibility studies of hydrogel synthesized via chemo-enzymatic route

Characterization and Biotechnological Potential of Intracellular Polyhydroxybutyrate by Stigeoclonium sp. B23 Using Cassava Peel as Carbon Source

Antibacterial Composite Materials Based on the Combination of Polyhydroxyalkanoates With Selenium and Strontium Co-substituted Hydroxyapatite for Bone Regeneration

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