Comparison of a quasi-dynamic and a static extraction method for the cytotoxic evaluation of acrylic bone cements

Hoess, Andreas; López, Alejandro; Engqvist, Håkan; Ott, Marjam Karlsson; Persson, Cecilia

doi:10.1016/j.msec.2016.01.048

Cited by 16 publications

(23 citation statements)

References 28 publications

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“…Once the polymerization is completed the cytotoxicity of the modified materials is comparable to their unmodified counterparts. This is in accordance with a previous study on modified PMMA cements, 23 where both a modification with castor oil, as well as a customized PMMA formulation (components acquired from Sigma-Aldrich) induced a cytotoxic effect at early time points, but not with 24 h extracts.…”

Section: Discussionsupporting

confidence: 93%

The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement

et al. 2016

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With the increasing elderly population an increase in the number of bony fractures associated to age-related diseases such as osteoporosis also follows. The relatively high stiffness of the acrylic bone cements used in these patients has been suggested to give raise to a suboptimal load distribution surrounding the cement in vivo, and hence contribute to clinical complications, such as additional fractures. The aim of this study was to develop a low-modulus bone cement, based on currently used, commercially available poly(methyl methacrylate) (PMMA) cements for vertebroplasty. To this end, acrylate end-functionalized oligo(trimethylene carbonate) (oTMC) was incorporated into the cements, and the resulting compressive mechanical properties were evaluated, as well as the cytotoxic and handling properties of selected formulations. Sixteen wt%oTMC was needed in the vertebroplastic cement Osteopal V to achieve an elastic modulus of 1063 MPa (SD 74), which gave a corresponding compressive strength of 46.1 MPa (SD 1.9). Cement extracts taken at 1 and 12 hours gave a reduced MG-63 cell viability in most cases, while extracts taken at 24 hours had no significant effect on cell behavior. The modification also gave an increase in setting time, from 14.7 min (SD 1.7) to 18.0 min (SD 0.9), and a decrease in maximum polymerization temperature, from 41.5°C (SD 3.4) to 30.7°C (SD 1.4). While further evaluation of other relevant properties, such as injectability and in vivo biocompatibility, remains to be done, the results presented herein are promising in terms of approaching clinically applicable bone cements with a lower stiffness.

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Section: Discussionsupporting

confidence: 93%

The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement

et al. 2016

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“…It is generally accepted that clinically successful BC should be a biocompatible material, but some bone cement components may be toxic to human body, such as unreacted methyl methacrylate (MMA) monomer or certain additives, i.e., N , N -dimethyl- p -toluidine, benzoyl peroxide, barium sulfate, as well as free radicals released during the polymerization process. The most recognized negative responses include tissue necrosis, fibrosis, or impaired bone remodeling in the vicinity of the implant [7,8,9]. When introduced into the body, BC may affect blood components, and some BCs used for implantation may affect hemostasis due to the hyperactivation or even inactivation of platelets [10].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration

et al. 2019

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Bacterial infections due to bone replacement surgeries require modifications of bone cement with antibacterial components. This study aimed to investigate whether the incorporation of gentamicin or nanometals into bone cement may reduce and to what extent bacterial growth without the loss of overall cytocompatibility and adverse effects in vitro. The bone cement Cemex was used as the base material, modified either with gentamicin sulfate or nanometals: Silver or copper. The inhibition of bacterial adhesion and growth was examined against five different bacterial strains along with integrity of erythrocytes, viability of blood platelets, and dental pulp stem cells. Bone cement modified with nanoAg or nanoCu revealed greater bactericidal effects and prevented the biofilm formation better compared to antibiotic-loaded bone cement. The cement containing nanoAg displayed good cytocompatibility without noticeable hemolysis of erythrocytes or blood platelet disfunction and good viability of dental pulp stem cells (DPSC). On the contrary, the nanoCu cement enhanced hemolysis of erythrocytes, reduced the platelets aggregation, and decreased DPSC viability. Based on these studies, we suggest the modification of bone cement with nanoAg may be a good strategy to provide improved implant fixative for bone regeneration purposes.

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“…Determination of the bending strength [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] of the sample (σi) in the software of the Bionix MTS system is based on the following equations: And the modulus of elasticity of the sample material, with the equation: (4) with: (5) where: y -the arrow that the test makes under the force of the machine [mm].…”

Section: Determination Of Bending Strengthmentioning

confidence: 99%

Experimental and Virtual Studies in Mechanical Applications of PP-RCT Pipes with Armored Materials

Enescu¹,

Stoian²,

Negrea³

et al. 2019

Mat.Plast.

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The new generation of newly developed materials, namely the high-crystallinity and high temperature polypropylene random copolymer, called PP-RCT, increases the crystalline capability of polypropylene, which allows pipes produced from this material to operate at higher pressures at elevated temperatures. Pressure tests on pipes made of PP-RCT materials show a resistance over a period of 50 years at 7000C (1580 F) 5 MPa (725 psi) compared with 3.2 MPa (464 psi) for standard materials PPR. By reinforcing PP-RCT with aluminum, glass and basalt composite results in a more resistant structure. As a result of the traction tests, it was found that in all three samples the tensile strength exceeds the manufacturer s limit (10 - 12.7 MPa), the values obtained being: st1 = 8 MPa - sample no. 1; st2 = 13 MPa - sample no. 2; st1 = 11 MPa - sample no. 3. The fiber glass insert is less resistant, the best variant being the basal insect sample (st2 = 13 MPa). Elongation at break for specimen no. 3 was recorded at 0.027 mm / mm. Tests on these material combinations were performed for stretching and flexing requests and compared to 3D created virtual models. Simulations of internal pressure behavior were also performed for different material configurations and the results were compared with the experimental data and the data supplied by the manufacturers.

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Comparison of a quasi-dynamic and a static extraction method for the cytotoxic evaluation of acrylic bone cements

Cited by 16 publications

References 28 publications

The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement

The effect of oligo(trimethylene carbonate) addition on the stiffness of acrylic bone cement

Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration

Experimental and Virtual Studies in Mechanical Applications of PP-RCT Pipes with Armored Materials

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