2016
DOI: 10.1099/jmm.0.000294
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Abstract: In this study, polyaminopropyl biguanide (PAPB) was compared to the molecularly closely related polyhexamethylene biguanide (PHMB) with respect to chemical relationship, antiseptic efficacy and cytotoxicity in vitro. Cytotoxicity for human keratinocytes (HaCaTs) and murine fibroblasts (L929) was determined according to ISO EN 10993-5 for both substances. Antimicrobial efficacy tests were performed via determination of the MBC, quantitative suspension method for substances and investigation of two PAPB-or PHMB-… Show more

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Cited by 38 publications
(26 citation statements)
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“…While PHMB was shown to be the second most cytotoxic biocide tested, it exhibited a relatively low rf value, resulting in the highest BI value out of all the test biocides. PHMB has previously shown low-level cytotoxicity toward mammalian cells, including L929 cells, which is suggested to be due to the interaction of the biocide with the mammalian cell membrane, leading to membrane damage (44). BAC was the second least cytotoxic biocide tested and showed the second highest level of antimicrobial activity in the presence of serum in the quantitative suspension tests.…”
Section: Discussionmentioning
confidence: 99%
“…While PHMB was shown to be the second most cytotoxic biocide tested, it exhibited a relatively low rf value, resulting in the highest BI value out of all the test biocides. PHMB has previously shown low-level cytotoxicity toward mammalian cells, including L929 cells, which is suggested to be due to the interaction of the biocide with the mammalian cell membrane, leading to membrane damage (44). BAC was the second least cytotoxic biocide tested and showed the second highest level of antimicrobial activity in the presence of serum in the quantitative suspension tests.…”
Section: Discussionmentioning
confidence: 99%
“…Product C also contained polyaminopropyl biguanide and citric acid in addition to potassium sorbate and sodium benzoate. Polyaminopropyl biguanides, which are polymeric biguanides consist of polycationic linear polymers with a hydrophobic backbone and multiple cationic groupings separated by methylene chains , are used widely to control microbes in pharmacological formulations and cosmetics . Their antibacterial action is believed to result from rapid binding of multiple positively charged domains to the negatively charged cell membranes of both Gram‐negative and Gram‐positive bacteria, which bridges acidic phospholipids through the hydrophobic methylene groups.…”
Section: Discussionmentioning
confidence: 99%
“…with respect to antiseptic efficacy and cytotoxicity in vitro. 32 Antimicrobial efficacy tests were performed via determination of the minimum bactericidal concentration. Polyaminopropyl Biguanide (PHMB; C6) exhibited high antimicrobial activity against Staphylococcus aureus and Escherichia coli (minimal bactericidal concentration 0.05 mg/mL [0.005%]), whereas polyaminopropyl biguanide (C3) proved to be ineffective in bacterial eradication.…”
Section: Johnson Et Al 33smentioning
confidence: 99%
“…Cytotoxicity was evaluated in human keratinocytes (HaCaTs) and murine fibroblasts (L929). 32 In fibroblast or keratinocyte cultures, concentrations for both test substances ranged from 0.005% to 1% vol/vol. Polyaminopropyl biguanide (C3) was also tested at concentrations ranging from 0.25% to 3% vol/vol.…”
Section: Johnson Et Al 33smentioning
confidence: 99%