Comparative transcriptome analysis identifies genes involved in the regulation of the pollen cytoskeleton in a genic male sterile wheat line

Wang, Yukun; Bai, Jianfang; Wang, Peng; Duan, Wenjing; Yuan, Shu; Zhang, Fengting; Gao, Shiqing; Liu, Lihuan; Pang, Bao-sen; Zhang, Liping; Zhao, Changping

doi:10.1007/s10725-018-0416-2

Cited by 12 publications

(11 citation statements)

References 54 publications

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“…After normalization, the false discovery rate (FDR) and fold change were calculated using the edgeR package for R (Wu et al, 2019). The differentially expressed genes (DEGs) were isolated with a log 2 fold change ≥1 or log 2 fold change ≤−1 and FDR < 0.05 (Wang et al, 2018). Gene Ontology (GO) term enrichment analysis of DEGs was carried out using Blast2GO (q-value ≤ 0.05).…”

Section: Rna-seqmentioning

confidence: 99%

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Morphological and Physiological Framework Underlying Plant Longevity in Arabidopsis thaliana

et al. 2020

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Section: Rna-seqmentioning

confidence: 99%

“…Gene Ontology (GO) term enrichment analysis of DEGs was carried out using Blast2GO (q-value ≤ 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was performed (q-value ≤ 0.05) using BlastX searches against the KEGG pathway database (Wang et al, 2018).…”

Section: Rna-seqmentioning

confidence: 99%

Morphological and Physiological Framework Underlying Plant Longevity in Arabidopsis thaliana

et al. 2020

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“…Based on previous reports, the fertility sensitive stage of BS366 was the meiosis stage of microspore mother cells [ 10 , 16 ]. Herein, we selected three key meiotic stages (1, 2, 3) for expression pattern analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Pollen cell staining was performed as described by Wang et al [ 10 ] and Xu et al [ 15 ]. Briefly, microfilaments and microtubules were respectively marked with tetramethylrhodamine isothiocyanate (TRITC)-phalloidin (Sigma, St. Louis, MO, USA) and anti-α-tubulin (mouse IgG monoclonal anti-α-tubulin, T-9026; Sigma) in pollen cells.…”

Section: Methodsmentioning

confidence: 99%

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Comprehensive analysis of formin gene family highlights candidate genes related to pollen cytoskeleton and male fertility in wheat (Triticum aestivum L.)

et al. 2021

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Background Formin, a highly conserved multi-domain protein, interacts with microfilaments and microtubules. Although specifically expressed formin genes in anthers are potentially significant in research on male sterility and hybrid wheat breeding, similar reports in wheat, especially in thermo-sensitive genic male sterile (TGMS) wheat, remain elusive. Results Herein, we systematically characterized the formin genes in TGMS wheat line BS366 named TaFormins (TaFHs) and predicted their functions in inducing stress response. In total, 25 TaFH genes were uncovered, majorly localized in 2A, 2B, and 2D chromosomes. According to the neighbor-joining (NJ) method, all TaFH proteins from wheat and other plants clustered in 6 sub-groups (A-F). The modeled 3D structures of TaFH1-A/B, TaFH2-A/B, TaFH3-A/B and TaFH3-B/D were validated. And different numbers of stress and hormone-responsive regulatory elements in their 1500 base pair promoter regions were contained in the TaFH genes copies. TaFHs had specific temporal and spatial expression characteristics, whereby TaFH1, TaFH4, and TaFH5 were expressed highly in the stamen of BS366. Besides, the accumulation of TaFHs was remarkably lower in a low-temperature sterile condition (Nanyang) than fertile condition (Beijing), particularly at the early stamen development stage. The pollen cytoskeleton of BS366 was abnormal in the three stages under sterile and fertile environments. Furthermore, under different stress levels, TaFHs expression could be induced by drought, salt, abscisic acid (ABA), salicylic acid (SA), methyl jasmonate (MeJA), indole-3-acetic acid (IAA), polyethylene glycol (PEG), and low temperature. Some miRNAs, including miR167, miR1120, and miR172, interacts with TaFH genes; thus, we constructed an interaction network between microRNAs, TaFHs, phytohormone responses, and distribution of cytoskeleton to reveal the regulatory association between upstream genes of TaFH family members and sterile. Conclusions Collectively, this comprehensive analysis provides novel insights into TaFHs and miRNA resources for wheat breeding. These findings are, therefore, valuable in understanding the mechanism of TGMS fertility conversion in wheat.

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Transcriptome Analysis of Sterile and Fertile Floral Buds from Recessive Genetic Male Sterility Lines in Watermelon (Citrullus lanatus L.)

Zhang

Ding²,

Wei

et al. 2022

J Plant Growth Regul

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Comparative transcriptome analysis identifies genes involved in the regulation of the pollen cytoskeleton in a genic male sterile wheat line

Cited by 12 publications

References 54 publications

Morphological and Physiological Framework Underlying Plant Longevity in Arabidopsis thaliana

Morphological and Physiological Framework Underlying Plant Longevity in Arabidopsis thaliana

Comprehensive analysis of formin gene family highlights candidate genes related to pollen cytoskeleton and male fertility in wheat (Triticum aestivum L.)

Transcriptome Analysis of Sterile and Fertile Floral Buds from Recessive Genetic Male Sterility Lines in Watermelon (Citrullus lanatus L.)

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