Comparative study on Manuka and Indonesian honeys to support the application of plasma jet during proliferative phase on wound healing

Wahyuningtyas, Eka Sakti; Iswara, Arya; Sari, Yunita; Kamal, Sodiq; Santosa, Budi; Ishijima, Tatsuo; Nakatani, Toshio; Putri, Indri Kartika; Nasruddin,

doi:10.1016/j.cpme.2018.08.001

Cited by 13 publications

(10 citation statements)

References 31 publications

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“…A complimentary report applied oil to the wound first, followed by Ar/air or Ar/O 2 plasma jet treatment, also reporting positive results [ 73 ]. In contrast, the combination of gas plasma jet wound treatment and medical honey did not lead to beneficial healing effects [ 62 , 63 ].…”

Section: Gas Plasma Treatment Of Skin and Wounds In Animal And Veterinary Modelsmentioning

confidence: 99%

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

2021

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The loss of skin integrity is inevitable in life. Wound healing is a necessary sequence of events to reconstitute the body’s integrity against potentially harmful environmental agents and restore homeostasis. Attempts to improve cutaneous wound healing are therefore as old as humanity itself. Furthermore, nowadays, targeting defective wound healing is of utmost importance in an aging society with underlying diseases such as diabetes and vascular insufficiencies being on the rise. Because chronic wounds’ etiology and specific traits differ, there is widespread polypragmasia in targeting non-healing conditions. Reactive oxygen and nitrogen species (ROS/RNS) are an overarching theme accompanying wound healing and its biological stages. ROS are signaling agents generated by phagocytes to inactivate pathogens. Although ROS/RNS’s central role in the biology of wound healing has long been appreciated, it was only until the recent decade that these agents were explicitly used to target defective wound healing using gas plasma technology. Gas plasma is a physical state of matter and is a partially ionized gas operated at body temperature which generates a plethora of ROS/RNS simultaneously in a spatiotemporally controlled manner. Animal models of wound healing have been vital in driving the development of these wound healing-promoting technologies, and this review summarizes the current knowledge and identifies open ends derived from in vivo wound models under gas plasma therapy. While gas plasma-assisted wound healing in humans has become well established in Europe, veterinary medicine is an emerging field with great potential to improve the lives of suffering animals.

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Section: Gas Plasma Treatment Of Skin and Wounds In Animal And Veterinary Modelsmentioning

confidence: 99%

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

2021

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“…Irradiation also degrades the chemical structure of polymers [ 26 , 27 ] and denatures the structure of proteins or other biopolymers [ 28 , 29 ]. In another study, Manuka and Indonesian honeys were compared for supporting the application of a plasma jet during the proliferative phase of wound healing [ 30 ]. In addition, Manuka honey was mixed with chitosan to prepare bioactive wound dressings that were more effective than commercial dressings [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Physically Crosslinked Chitosan/PVA Hydrogels Containing Honey and Allantoin with Long-Term Biocompatibility for Skin Wound Repair: An In Vitro and In Vivo Study

Koosha

Aalipour

Shirazi

et al. 2021

JFB

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Chitosan/PVA hydrogel films crosslinked by the freeze–thaw method and containing honey and allantoin were prepared for application as wound dressing materials. The effects of the freeze–thaw process and the addition of honey and allantoin on the swelling, the gel content and the mechanical properties of the samples were evaluated. The physicochemical properties of the samples, with and without the freeze–thaw process, were compared using FTIR, DSC and XRD. The results showed that the freeze–thaw process can increase the crystallinity and thermal stability of chitosan/PVA films. The freeze–thaw process increased the gel content but did not have a significant effect on the tensile strength. The presence of honey reduced the swelling and the tensile strength of the hydrogels due to hydrogen bonding interactions with PVA and chitosan chains. Long-term cell culture experiments using normal human dermal fibroblast (NHDF) cells showed that the hydrogels maintained their biocompatibility, and the cells showed extended morphology on the surface of the hydrogels for more than 30 days. The presence of honey significantly increased the biocompatibility of the hydrogels. The release of allantoin from the hydrogel was studied and, according to the Korsmeyer–Peppas and Weibull models, the mechanism was mainly diffusional. The results for the antimicrobial activity against E. coli and S. aureus bacteria showed that the allantoin-containing samples had a more remarkable antibacterial activity against S. aureus. According to the wound healing experiments, 98% of the wound area treated by the chitosan/PVA/honey hydrogel was closed, compared to 89% for the control. The results of this study suggest that the freeze–thaw process is a non-toxic crosslinking method for the preparation of chitosan/PVA hydrogels with long term biocompatibility that can be applied for wound healing and skin tissue engineering.

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“…11,12 Abnormal epidermal tissue in skin wounds was found to be due to the contact style of plasma jet treatment. 13 However, applying plasma jet treatment in the contact style may effectively eradicate chronic wound-related bacteria. 14−16 Plasma jet treatment is significantly effective in killing bacteria including S. aureus, Pseudomonas aeruginosa, MRSA, and methicillin-resistant Staphylococcus epidermidis (MRSE).…”

Section: Introductionmentioning

confidence: 99%

Accelerated Healing of Chronic Wounds under a Combinatorial Therapeutic Regimen Based on Cold Atmospheric Plasma Jet Using Contact and Noncontact Styles

et al. 2021

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One critical element for applying atmospheric pressure plasma jet for medical purposes is that it is possible to construct a combinatorial therapeutic regimen based on contact and noncontact styles for the cold atmospheric plasma jet. This study evaluates plasma jet effectiveness for bacteria-infected wounds in a small animal model. In this investigation, we test a novel combinative treatment using contact and noncontact style for plasma jet that was generated at high voltage of ~ 9 kV. We use medical-grade argon gas as a single carrier gas. The object of plasma treatment is BALB/c mouse skin wounds that were infected with Staphylococcus aureus. We use four plasma jet treatments, namely, C (control), CP-CP (contact), NCP-NCP (noncontact), and CP-NCP (contact-noncontact). For CP-NCP, from days 0 to 7 we apply a contact style of plasma jet treatment to wounds to kill bacteria; from days 8 to 13, a noncontact style of plasma jet is applied to stimulate wound healing. Our results show that with CP-CP, contact plasma treatment can remove the biofilm layer, but after the biofilm layer disappears contact plasma treatment inhibits the wound-healing process. NCP-NCP is not effective in eliminating bacterial biofilms and impedes the wound-healing process. With CP-NCP, contact plasma exposure during days 0 to 7 is able to remove bacterial biofilms, and irradiation of noncontact plasma during days 8 to 14 accelerates wound healing. Finally, CP-NCP significantly accelerates healing. The combinatorial therapeutic regimen based on contact and noncontact styles of cold atmospheric plasma jet is recommended for chronic wound management, because it effectively removes bacterial biofilms and accelerates wound healing.

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Comparative study on Manuka and Indonesian honeys to support the application of plasma jet during proliferative phase on wound healing

Cited by 13 publications

References 31 publications

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

Physically Crosslinked Chitosan/PVA Hydrogels Containing Honey and Allantoin with Long-Term Biocompatibility for Skin Wound Repair: An In Vitro and In Vivo Study

Accelerated Healing of Chronic Wounds under a Combinatorial Therapeutic Regimen Based on Cold Atmospheric Plasma Jet Using Contact and Noncontact Styles

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