Comparative Study of Electrochemical Biosensors Based on Highly Efficient Mesoporous ZrO₂-Ag-G-SiO₂ and In₂O₃-G-SiO₂ for Rapid Recognition of E. coli O157:H7

Abstract

Here, we reported an innovative and electrochemical biosensor for the rapid detection of Escherichia coli O157:H 7. We fabricated the mesoporous ZrO 2 -Ag-G-SiO 2 (ZAGS) and In 2 O 3 -G-SiO 2 (IGS) sensors, and cyclic voltammetry (CV) was employed to detect the bacteria. The development of these portable sensors addresses the challenges of conventional time-consuming and more expensive laboratory-based analyses. Hence, the biosensors were highly selective to detect E. coli . The sensor could recognize an individual E. coli cell in 1 μL of sample volume within 30 s. E. coli live cells tied down on sample nanoparticles worked toward the definite acquirement of E. coli . The high thickness of negative charge on the surface of E. coli cells effectively regulated the concentration of dominant part charge carriers in the mesoporous channel, allowing a continuous check of E. coli concentration in a known sample. The signal current decreased linearly, while the E. coli concentration increased from 1.0 × 10 1 to 1.0 × 10 10 CFU/mL. ZAGS and IGS biosensors could detect E. coli in the range from 10 1 to 10 10 CFU/mL. ZAGS and IGS biosensors in this investigation showed great specificity, reproducibility, stability, and selectivity and are expected to have a great impact on applications in the detection of foodborne pathogens.

Advances in Nanomaterials-Based Electrochemical Biosensors for Foodborne Pathogen Detection

“…Especially, nanomaterials with intrinsic conductivity, such as metal oxide, carbon nanomaterials and metal nanoparticles, significantly improve the sensing devices that relied on electrical signal. Moreover, synergic effects can be achieved by combining two or more 0-3D nanomaterials, forming a nanocomposite heterostructure on the same electrode [45][46][47][48]. Different surface modifications involving thiols, amines and silanes are performed to functionalize electrodes carrying nanomaterials in order to attach biorecognition elements to the electrode in a stable way.…”

“…Recent research has focused on nanocomposite heterostuctures, where different characteristics of each component lead to improved performance and characteristics of electrochemical biosensors for food pathogen detection [46]. Metal oxide 0-3D nanomaterials have great potential to improve the biorecognition performance, where focus is on engineering the microstructure, as shown by Zhai et al, where a 3D networked carbon nanowall/diamond supporting CuO architecture was developed combining microwave plasma chemical vapor deposition of the hybrid carbon nanowall/diamond film on fluorine tin oxide (FTO) glass substrate.…”

“…It was then used as a template for deposition of Cu nanoparticles by magnetron sputtering followed by growth of CuO nanoparticles by an electrochemical method [45]. Fatema et al performed a comparative study of two mesoporous nanocomposites, ZrO 2 -Ag-G-SiO 2 and In 2 O 3 -G-SiO 2 (Figure 3), for rapid and highly efficient detection of Escherichia coli using cyclic voltammetry, achieving detection in the range from 10 1 CFU/mL to 10 10 CFU/mL [46]. Several reports have indicated significant sensing improvements obtained by using metal oxide nanoparticles in electrochemical biosensors.…”

“…Carbon nanomaterials are often used as one of the components in nanocomposite electrochemical biosensors for foodborne pathogen detection. For example, grapheme oxide as part of mesoporous nanocomposite for detection of E.coli [46]. A rapid and sensitive detection in the dynamic range from 10 1 CFU/mL to 10 8 CFU/mL with a detection limit of 10 1 CFU/mL of S. enterica was achieved with a nanocomposite of rGO and CNT modified with an amino-modified DNA aptamer [124].…”

3D Modeling of Silver Doped ZrO2 Coupled Graphene-Based Mesoporous Silica Quaternary Nanocomposite for a Nonenzymatic Glucose Sensing Effects

“…Electrochemistry, fluorescence, colorimetry, photoelectrochemistry, and chemical luminescence, have been received for glucose sensing [1,2]. Among them, the electrochemical detecting method has gotten high attention due to its high affectability, promising reaction time [3][4][5]. Glucose oxidation on the sensor is dependable for the chemisorption of the hydroxyl group onto the metal oxide and shaping the bond among the d-electron of metal and glucose atoms.…”