Comparative study between univariate spectrophotometry and multivariate calibration as analytical tools for quantitation of Benazepril alone and in combination with Amlodipine

Farouk, Maha; Elaziz, Omar Abd; Tawakkol, Shereen M.; Hemdan, A.; Shehata, Mohamed

doi:10.1016/j.saa.2013.12.094

Cited by 15 publications

(6 citation statements)

References 27 publications

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“…BEN and AML combination was reported to be analyzed by several techniques. These reports proposed several spectrophotometric methods [3][4][5][6][7], high-performance liquid chromatography (HPLC) methods [7][8][9][10][11][12][13][14][15], and only 2 highperformance thin-layer chromatography (HPTLC) methods [16,17]. 6-Chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7sulfonamide 1,1-dioxide HCT is a diuretic of moderate potency [2].…”

Section: Introductionmentioning

confidence: 99%

Validated capillary zone electrophoretic method for simultaneous analysis of benazepril in combination with amlodipine besylate and hydrochlorothiazide

Ahmed

Belal

Shaalan

et al. 2020

Acta Chromatographica

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In this work, a novel, simple, and quick capillary zone electrophoresis (CZE) method was proposed for simultaneous analysis of benazepril (BEN) with other co-administrated antihypertensive drugs, amlodipine besylate (AML) and hydrochlorothiazide (HCT), using a diode array detector (DAD). A fused silica capillary (78.5 cm total length, 70 cm effective length, and 75 μm id) was used in separation using a 40 mM phosphate buffer pH 7.5 as a running background electrolyte (BGE) under a positive potential of 30 KV, at a stable temperature of 25 °C for capillary during separation. Hydrodynamic injections were performed for 12 s at 50 mbar, and detection was performed at 210 nm for AML and BEN, at 225 nm for HCT, and at 232 nm for xipamide (XIP) added as an internal standard (IS). Separation of the three analyzed drugs and the IS was performed in less than 8 min. Migration times were 4.06, 5.23, 6.69, and 7.3 min for AML, HCT, BEN, and XIP, respectively. The findings proved that the proposed method was linear in the range of 10–80 μg/mL for all drugs with correlation coefficients >0.9994. The limit of detection (LOD) values of AML, HCT, and BEN were 1.004, 1.224, and 0.896 μg/mL, respectively, whereas the limit of quantification (LOQ) values were 3.124, 3.727, and 2.749 μg/mL for the cited drugs, respectively. Peak identity and purity were confirmed by DAD. The developed CZE method was applied for the analysis of the three antihypertensive drugs successfully in their combined pharmaceutical tablets, and it can be used for the quality control of single-pill combination (SPC) samples of these drugs in short time.

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Section: Introductionmentioning

confidence: 99%

Validated capillary zone electrophoretic method for simultaneous analysis of benazepril in combination with amlodipine besylate and hydrochlorothiazide

Ahmed

Belal

Shaalan

et al. 2020

Acta Chromatographica

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“…The ratio subtraction method shows that this method is very simple, accurate, and does not require complicated mathematical calculations using only constants in the spectrum of ratios that can be applied to the IRB and HCT levels. [16], [17], [18].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Estimation of Anti Hypertension Combination by Ratio Subtraction Spectrophotometry Method

Bachri

Masfria

Syahputra

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND:Irbesartan and hydrochlorothiazide are a group of anti-hypertensive drugs that are very effective and safe to use to reduce blood pressure and oedema. The combination has a small active ingredient content so that if the treatment didn’t meet the requirements for therapeutic doses, it not achieved to the maximum therapy.AIM:The research aims to the simultaneous determination of irbesartan and hydrochlorothiazide in tablets by Ratio subtraction spectrophotometry method.METHODS:The absorption spectra and sample measurement in the Ratio subtraction method performed on Irbesartan at a wavelength of 247.6 nm and 273.6 nm for the Hydrochlorothiazide (HCT) using 0.1 N NaOH as a solution. This method is validated with linearity, accuracy, and precision in intraday and interday, LOD and LOQ and applied in the determination of a mixture of irbesartan and hydrochlorothiazide in the dosage tablet.RESULTS:The validation test for IRB is 101.03 for accuracy, with a precision of 0.57; with precision testing at intraday 0.34 and interday 1.34, and LOD is 0.70 and LOQ is 2.12. Meanwhile, validation for HCT that the accuracy 100.34%; precision 0.89 and precision on intraday 1.20 and interday 1.18, and LOD 0.78 and LOQ 2.37 with IRB levels are 101.03 ± 0.63% and HCT is 100.59 ± 0.91%.CONCLUSION:The ultraviolet spectrophotometric method in subtraction ratio method was validated a method of linearity, accuracy, precision in intraday and interday, LOD, and LOQ and according to ICH guidelines and successfully applied for the determination simultaneous of irbesartan and hydrochlorothiazide in the tablet’s dosage form.

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“…Few analytical methods for the determination of amlodipine besylate and benazepril hydrochloride in combined dosage forms including RP-HPLC [4][5][6], spectrophotometry [6,7] and HPTLC [8] have been reported. Several methods have been reported for the determination of amlodipine with other drugs by UV-spectrophotometry [9][10][11][12][13], dual wavelength spectrophotometry [14,15], derivative spectrophotometry [16,17], kinetic spectrophotometry [18], HPLC with UV detection [17,[19][20][21][22][23][24][25][26][27], HPLC with PDA detection [28][29][30], HPLC with fluorescence detection [31], spectrofluorimetry [32], HPTLC [33] and capillary electrophoresis [34] and amlodipine alone by visible spectrophotometry [35,36] and voltammetry [37] from pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

A Validated Stability-Indicating Liquid Chromatographic Method for the Simultaneous Determination of Amlodipine and Benazepril in Capsules Dosage Form

2014

Can Chem Trans

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A simple, sensitive, reproducible and specific stability indicating high performance liquid chromatographic method for the simultaneous estimation of amlodipine besylate (AMD) and benazepril hydrochloride (BEZ) in their combined drug product was developed and validated. Risperidone (RIS) was used as internal standard. AMD and BEZ were separated and quantitated on a Hichrom 5 C8 column (250 × 4.6 mm i.d., 5 μm particle size) using a mixture of methanol-formic acid 0.1 M (63:37, v/v) as a mobile phase and at a flow rate of 0.9 mL min-1. The retention times were about 3.7, 5.3 and 7.3 min for RIS, BEZ and AMD, respectively. Quantification was achieved with an UV detector at 242 nm over the concentration ranges of 0.85-807.0 μg mL-1 for BEZ and 0.32-785.0 μg mL-1 for AMD. The limits of detection were 0.18 and 0.12 μg mL-1 for BEZ and AMD, respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. AMD and BEZ stock solutions were subjected to different stress conditions. The degraded product peaks were well resolved from the pure drug peak with significant difference in their retention time values. Stressed samples were assayed using developed HPLC method. The method is also demonstrated to be robust, because it is resistant to small variations of chromatographic variables such as pH, mobile phase composition and flow rate. The proposed method is found to be very sensitive and accurate for the determination of AMD and BEZ in combined capsule dosage form.

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Comparative study between univariate spectrophotometry and multivariate calibration as analytical tools for quantitation of Benazepril alone and in combination with Amlodipine

Cited by 15 publications

References 27 publications

Validated capillary zone electrophoretic method for simultaneous analysis of benazepril in combination with amlodipine besylate and hydrochlorothiazide

Validated capillary zone electrophoretic method for simultaneous analysis of benazepril in combination with amlodipine besylate and hydrochlorothiazide

Quantitative Estimation of Anti Hypertension Combination by Ratio Subtraction Spectrophotometry Method

A Validated Stability-Indicating Liquid Chromatographic Method for the Simultaneous Determination of Amlodipine and Benazepril in Capsules Dosage Form

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