Quantitative Estimation of Anti Hypertension Combination by Ratio Subtraction Spectrophotometry Method

Abstract

BACKGROUND:Irbesartan and hydrochlorothiazide are a group of anti-hypertensive drugs that are very effective and safe to use to reduce blood pressure and oedema. The combination has a small active ingredient content so that if the treatment didn’t meet the requirements for therapeutic doses, it not achieved to the maximum therapy.AIM:The research aims to the simultaneous determination of irbesartan and hydrochlorothiazide in tablets by Ratio subtraction spectrophotometry method.METHODS:The absorption spectra and sample measurement in the Ratio subtraction method performed on Irbesartan at a wavelength of 247.6 nm and 273.6 nm for the Hydrochlorothiazide (HCT) using 0.1 N NaOH as a solution. This method is validated with linearity, accuracy, and precision in intraday and interday, LOD and LOQ and applied in the determination of a mixture of irbesartan and hydrochlorothiazide in the dosage tablet.RESULTS:The validation test for IRB is 101.03 for accuracy, with a precision of 0.57; with precision testing at intraday 0.34 and interday 1.34, and LOD is 0.70 and LOQ is 2.12. Meanwhile, validation for HCT that the accuracy 100.34%; precision 0.89 and precision on intraday 1.20 and interday 1.18, and LOD 0.78 and LOQ 2.37 with IRB levels are 101.03 ± 0.63% and HCT is 100.59 ± 0.91%.CONCLUSION:The ultraviolet spectrophotometric method in subtraction ratio method was validated a method of linearity, accuracy, precision in intraday and interday, LOD, and LOQ and according to ICH guidelines and successfully applied for the determination simultaneous of irbesartan and hydrochlorothiazide in the tablet’s dosage form.

Simultaneous Determination of Isoniazid and Pyridoxine Hydrochloride in Tablet Dosage Forms using Ratio Subtraction Spectrophotometry

“…This analysis assessed the statistical significance of the results, that the relationship between absorbance and concentration is linear [16], meaning the ratio subtraction spectrophotometry method can be used in this study. Figure 3 shows that the spectral overlap of INH (15 µg/mL), PRD (18 µg/mL), and mixture (INH 15 µg/mL and PRD 18 µg/mL) was overcome by using the ratio subtraction spectrophotometry method [15].…”

“…This analysis of wavelength usage for analysis indicates that the initial divisor is used for the ratio subtraction approach, where the initial divisor is employed in the next step [15,16]…”

Simultaneous Content Analysis of Rifampicin, Isoniazid, and Pyrazinamide in Tablet Dosage Form by Spectrophotometry Ultraviolet With DWM and RSM

“…The concentration of the divisor was determined from a concentration range that satisfied the Lambert-Beer law. The spectral overlaps of the ratio spectrum of RFP, INH, and PRZ were obtained from various concentrations (Bachri et al, 2019).…”

Method Development for Simultaneously Determining Indomethacin and Nicotinamide in New Combination in Oral Dosage Formulations and Co-Amorphous Systems Using Three UV Spectrophotometric Techniques

“…In total, three UV spectrophotometric methods were developed. Tese methods have been proven to be accurate, precise, fast, simple, and eco-friendly for many combinations and multicomponent dosage formulations without using any hazardous solvents or invasive materials [30][31][32][33][34][35]. Additionally, more advanced UV spectrophotometric methods have been developed initially from these reported ones deploying further mathematical processing [36][37][38].…”