Comparative production of glucose and high fructose syrup from cassava and sweet potato roots by direct conversion techniques

Johnson, Regy; Padmaja, G.; Moorthy, S. N.

doi:10.1016/j.ifset.2009.04.001

Cited by 48 publications

(31 citation statements)

References 15 publications

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“…Además, se emplean en la industria química para la producción de alcohol (2, 3) y tienen aplicabilidad en la industria farmacéutica para la elaboración de vacunas y antibióticos (4). Aunque el almidón de maíz es la principal materia prima para la producción de jarabes de glucosa, la yuca ha sido considerada como fuente alternativa debido a sus altos contenidos de almidón (87,67% en base seca) (5,6). La hidrólisis del almidón se hace comúnmente por vía enzimática, obteniéndose hidrolizados hasta con 95 ED o superior (7).…”

Section: Introductionunclassified

Estudio Del Proceso De Producción en Continuo De Hidrolizados De Almidón De Yuca Integrando Hidrólisis Enzimática Y Clarificación Con Membranas

Salcedo

Ricardo

Medina

2017

Vitae

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RESUMENAntecedentes: Los jarabes de glucosa obtenidos a partir del almidón de yuca han sido estudiados debido al alto contenido de almidón que su materia prima presenta (87,67 % base seca). El proceso de producción se realiza convencionalmente por vía enzimática en reactores batch (en dos etapas), seguida de una etapa de clarificación para remover sólidos suspendidos e impurezas insolubles; esta configuración aumenta los costos de producción y lo hace menos productivo. Objetivos: Estudiar el proceso de producción en continuo de hidrolizados de almidón de yuca integrando hidrólisis enzimática (en una sola etapa) y clarificación con tecnología de membranas. Métodos: Se utilizaron membranas cerámicas tubulares monocanal TAMI InsideCeram ® de 50 kDa y 0,2 μm de umbral de corte. Se analizó el efecto de la concentración de enzima (α-amilasa: 0,028-0,112 % p/p; glucoamilasa: 0,0315-0,126 % p/p) en los hidrolizados producidos en una sola etapa, además de evaluar el efecto del umbral de corte en la producción de hidrolizados bajo la configuración en continuo. Se determinaron permeabilidades, y los hidrolizados y sus filtrados fueron caracterizados en turbidez, ED, % glucosa, % materia seca y °Brix. Resultados: Se encontró que las características de los hidrolizados no se ven afectadas significativamente por las concentraciones de enzima evaluadas en mezcla, permitiendo trabajar con la menor relación. Los resultados para continuo mostraron que es posible trabajar con la membrana de 0,2 μm sin afectar la calidad del producto, haciendo el proceso más productivo. Conclusiones: Se comprobó las ventajas de utilizar una configuración en continuo respecto a una en batch, distanciando tiempos de parada, manteniendo la calidad del producto, y aumentando la productividad.Palabras clave: Filtración, membranas, hidrólisis, almidón, yuca. ABSTRACTBackground: Glucose syrups obtained from cassava starch have been studied due to the high content of starch that their raw material presents (87.67% dry base). The production process is performed conventionally in batch reactors using enzymes (in two steps). Subsequently, the hydrolysates produced are clarified to

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Section: Introductionunclassified

Estudio Del Proceso De Producción en Continuo De Hidrolizados De Almidón De Yuca Integrando Hidrólisis Enzimática Y Clarificación Con Membranas

Salcedo

Ricardo

Medina

2017

Vitae

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“…Growthstimulating compounds within sweet potato peel are carbohydrates, amino acids, vitamins, minerals, and dietary fibers which directly affect the growth of bacterial cells by providing most essential nitrogen and carbon components for cell wall synthesis and proceed metabolic machinery of microbial cells for enzyme synthesis (Gyawali and Ibrahim 2012;Padmaja 2009;Broiheir 2006;Bibi et al 2014). Sweet potato peel is not only used for the production of enzymes but it can also be directly hydrolyzed to sugar syrups by amylolytic proteins and utilized in food, chocolate, and confectionery industries to make juices, sweets, and fermented products such as citric acid, gluconic acid, and ethanol (Johnson et al 2009;Hull 2010). Interestingly, it was noticed that the production of α-1,4-glucosidase was decreased in all other tested carbon sources including barley, wheat starch, and sugarcane bagasse (2037.1 ± 101.8, 1481.5 ± 74.0, 1111.0 ± 55.5 U ml −1 ) as compared to sweet potato peel.…”

Section: Resultsmentioning

confidence: 99%

Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel

Nawaz

Bibi

Karim

et al. 2016

Environ Sci Pollut Res

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In the current study, sweet potato peel (Ipomoea batatas) was observed as the most favorable substrate for the maximum synthesis of α-1,4-glucosidase among various agro-industrial residues. Bacillus licheniformis KIBGE-IB4 produced 6533.0 U ml of α-1,4-glucosidase when growth medium was supplemented with 1% dried and crushed sweet potato peel. It was evident from the results that bacterial isolate secreted 6539.0 U ml of α-1,4-glucosidase in the presence of 0.4% peptone and meat extract with 0.1% yeast extract. B. licheniformis KIBGE-IB4 released 6739.0 and 7190.0 U ml of enzyme at 40 °C and pH 7.0, respectively. An improved and cost-effective growth medium design resulted 8590.0 U ml of α-1,4-glucosidase with 1.3-fold increase as compared to initial amount from B. licheniformis KIBGE-IB4. This enzyme can be used to fulfill the accelerating demand of food and pharmaceutical industries. Further purification and immobilization of this enzyme can also enhance its utility for various commercial applications. Graphical abstract Pictorial representation of maltase production from sweet potato peel.

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“…Several authors had investigated the enzymatic hydrolysis of numerous tuber starches. These studies included the hydrolysis profiles of native and mildly heat-treated tapioca and sweet potato starches at sub gelatinization temperature [11], direct conversion of cassava and sweet potato roots slurry into glucose and high fructose syrups [12], and the production of glucose and high fructose syrup from various tuber starches [13]. The hydrolyzed starches were chemically analyzed for their degree of hydrolysis, which was also further physically proven by the presence of distinct pores penetrating deep into the starch granules [14].…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Cyanide on the Activity of Granular Starch Hydrolyzing Enzyme (GSHE) during Hydrolysis of Cassava (Manihot Esculenta Crantz) Starch

Hargono

Kumoro

Jos

2018

Period. Polytech. Chem. Eng.

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The kinetics and inhibitory effects of cyanide on the granular starch hydrolyzing enzyme (GSHE) activity during hydrolysis of cassava (Manihot esculenta Crantz) starch at low temperature were studied. The substrates included native cassava starch at various concentrations (100-400 g/L) and native cassava starches with added cyanide at various concentrations (50-150 mg/kg), while the concentration of enzyme was 1.5% (w/w). A decrease in reducing sugar concentration during hydrolysis of cassava starch indicated that the cyanide reduced the enzyme activity. Lineweaver-Burk plot of Michaelis-Menten equation was used to study the inhibition kinetics. The maximum velocity (V max ) value was higher for native cassava starch than that of native cassava starch with added cyanides.The presence of cyanide was found to reduce the V max values. No significant different of the saturation constant (K m ) value between native cassava starch and native cassava starch with added cyanides was observed. Based on the inhibition type analysis, the effect of cyanide in the cassava starch can be classified as a noncompetitive inhibition, with the K i value of 0.33 mg/L.

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Comparative production of glucose and high fructose syrup from cassava and sweet potato roots by direct conversion techniques

Cited by 48 publications

References 15 publications

Estudio Del Proceso De Producción en Continuo De Hidrolizados De Almidón De Yuca Integrando Hidrólisis Enzimática Y Clarificación Con Membranas

Estudio Del Proceso De Producción en Continuo De Hidrolizados De Almidón De Yuca Integrando Hidrólisis Enzimática Y Clarificación Con Membranas

Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel

Inhibitory Effects of Cyanide on the Activity of Granular Starch Hydrolyzing Enzyme (GSHE) during Hydrolysis of Cassava (Manihot Esculenta Crantz) Starch

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