Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel

Abstract

In the current study, sweet potato peel (Ipomoea batatas) was observed as the most favorable substrate for the maximum synthesis of α-1,4-glucosidase among various agro-industrial residues. Bacillus licheniformis KIBGE-IB4 produced 6533.0 U ml of α-1,4-glucosidase when growth medium was supplemented with 1% dried and crushed sweet potato peel. It was evident from the results that bacterial isolate secreted 6539.0 U ml of α-1,4-glucosidase in the presence of 0.4% peptone and meat extract with 0.1% yeast extract. B. licheniformis KIBGE-IB4 released 6739.0 and 7190.0 U ml of enzyme at 40 °C and pH 7.0, respectively. An improved and cost-effective growth medium design resulted 8590.0 U ml of α-1,4-glucosidase with 1.3-fold increase as compared to initial amount from B. licheniformis KIBGE-IB4. This enzyme can be used to fulfill the accelerating demand of food and pharmaceutical industries. Further purification and immobilization of this enzyme can also enhance its utility for various commercial applications. Graphical abstract Pictorial representation of maltase production from sweet potato peel.

Avaliação Das Coordenadas Colorimétricas De Leites Uht Com Baixo Teor De Lactose

“…A literatura apresenta que as cascas de bata-doce são mais ricas nutricionalmente que a polpa, e são fonte potencial de compostos bioativos, incluindo antioxidantes, fibras dietéticas, pigmentos (como os carotenoides), o que a torna um alimento que traz benefícios para a saúde, pois os compostos bioativos presentes atuam como antioxidantes, hepatoprotectores, antiinflamatórios, antitumorais, antidiabéticos, antimicrobianos, antiobesidade e antienvelhecimento (NAWAZ et al, 2016;WU, 2016;PATHAK et al, 2017).…”

“…De modo geral, os resíduos industriais poluem o meio ambiente (NAWAZ et al, 2016). Segundo Pathak et al (2017), a cada tonelada de batata processada 0,16 toneladas de resíduos são gerados.…”

“…Por isso, faz-se necessário destinar esses resíduos sólidos para utilização, já que possuem valor nutricional. A sua utilização além de ajudar a reduzir a poluição, contribui para produção de enzimas e para o fornecimento de nutrientes (NAWAZ et al, 2016).…”

Peer Review #1 of "Starch biotransformation into isomaltooligosaccharides using thermostable alpha-glucosidase from Geobacillus stearothermophilus (v0.2)"

“…Among the alpha-glucosidases that have been studied, the vast majority are derived from microorganisms while only a few are from plants and animals. Aspergillus niger (Liu et al, 2013;Hatano et al, 2017), Aspergills foetidus, Bacillus licheniformis (Nawaz et al, 2014;Nawaz et al, 2016), Pseudoalteromonas sp. (Li et al, 2016), Podospora anserina (Song et al, 2013), Monascus rubervan Tieghem (Chen and Xie, 2008), crenarchaeon Sulfolobus tokodaii (Park et al, 2013), Patinopecten yessoensis (Masuda et al, 2016), Geobacillus sp (Hung et al, 2005)., Malbranchea cinnamomea (Yan et al, 2015), Saccharomyces cerevisiae (Hossain et al, 2016), Xanthophyllomyces dendrorhous (Gutierrez-Alonso et al, 2016), Thermus thermophilus (Zhou et al, 2015), rice seed (Kim et al, 2016), Bifidobacterium adolescentis, Geobacillus stearothermophilus (G. stearothermophilus), Bombyx mori, and Spodoptera frugiperda PeerJ reviewing PDF | (2018:01:23093:2:2:CHECK 2 Jun 2018)…”

Starch biotransformation into isomaltooligosaccharides using thermostable alpha-glucosidase from Geobacillus stearothermophilus

“…Among the alpha-glucosidases that have been studied, the vast majority are derived from microorganisms while only a few are from plants and animals. Aspergillus niger ( Liu et al, 2013 ; Hatano et al, 2017 ), Aspergills foetidus, Bacillus licheniformis ( Nawaz et al, 2014 ; Nawaz et al, 2016 ), Pseudoalteromonas sp. ( Li et al, 2016 ), Podospora anserina ( Song et al, 2013 ), Monascus rubervan Tieghem ( Chen & Xie, 2008 ), crenarchaeon Sulfolobus tokodaii ( Park et al, 2013 ) , Patinopecten yessoensis ( Masuda et al, 2016 ) , Geobacillus sp ( Hung et al, 2005 ), Malbranchea cinnamomea ( Yan et al, 2015 ), Saccharomyces cerevisiae ( Hossain et al, 2016 ) , Xanthophyllomyces dendrorhous ( Gutierrez-Alonso et al, 2016 ) , Thermus thermophilus ( Zhou, Xue & Ma, 2015 ) , rice seed ( Kim et al, 2016 ), Bifidobacterium adolescentis , Geobacillus stearothermophilus , Bombyx mori , and Spodoptera frugiperda ( Watanabe et al, 2013 ) secrete alpha-glucosidase.…”