Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn. – An in vitro approach

Rajan, Murugan; Thangaraj, Parimelazhagan

doi:10.1016/j.jksus.2013.09.006

Cited by 150 publications

(82 citation statements)

References 28 publications

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“…From the data obtained, successive Soxhlet acetone extract of aerial parts has a highest percentage of inhibition followed by fruit methanol extract. This result was in concordance with the study of Murugan and Parimelazhagan 40 where successive Soxhlet methanol extract of Osbeckia parvifolia has the ability to protect the protein membrane from heat and alkali induced protein denaturation comparable to diclofenac. Values are mean± SD, n=3.…”

Section: In Vitro Anti-inflammatory Activity: Inhibition Of Protein Dsupporting

confidence: 92%

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2017

IJPSR

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Aristolochia longa L. (Aristolochiacea) is a native plant of Algeria used in traditional medicine. This study was devoted to the determination of polyphenols, flavonoids, and tannins contents of A. longa L. extracts. Extracts were prepared from aerial parts (stems and leaves), fruits and tubers by using various solvents with different polarities such as acetone, methanol and distilled water. Acetone extracts from the aerial parts presented the highest contents of polyphenols (525.43±29.6 µg/mg) followed by fruit aqueous extract (518.54±14.93 µg/mg), while the aerial parts methanol extract shown the highest flavonoid content (52.37±0.94 µg/mg) and exhibited the highest antioxidant capacity of DPPH and reducing power (55.04 ±1.29 µg/mL and 0.2±0.019 mg/mL, respectively), therefore the aerial parts acetone extract showed the highest antioxidant capacity in the β-carotene bleaching inhibition test with 57%. For antimicrobial activity, the fruit methanol extract was too efficient against the bacterial strains tested, whereas no effect was observed when these extracts were tested against fungi. The protein denaturation was found in the aerial parts acetone extract to be 78.35±6.18% followed by fruit methanol extract, 68.04±4.72% at the dose 500 µg/mL, with regards to standards diclofenac sodium. These preliminary results could be used to justify the traditional use of this plant and its bioactive substances could be exploited for therapeutic purposes.

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Section: In Vitro Anti-inflammatory Activity: Inhibition Of Protein Dsupporting

confidence: 92%

“…Production of autoantigen in certain arthritic diseases may be due to denaturation of protein 40 . The mechanism of denaturation probably involves alteration I electrostatic hydrogen, hydrophobic and disulfid bonding 41 .…”

Section: In Vitro Anti-inflammatory Activity: Inhibition Of Protein Dmentioning

confidence: 99%

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2017

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“…According to the results, the effective solvent used to extract flavonoid content appeared on ethanol solvent (Table 1). This may indicate that sequential extraction using ethanol solvent is the most appropriate to use for extractability of flavonoid content from P. cablin [31].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Antimicrobial Activities ofPogostemon cablin(Blanco) Benth.

et al. 2017

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Pogostemon cablin (Lamiaceae) has been widely used in traditional medicine. In this study, the antioxidant and antimicrobial activities of leaves from P. cablin extracts were investigated. The water extracts had the highest total phenolic content 116.88±0.48 mg gallic acid equivalent/g of dry plant extract. Nevertheless, high levels of total flavonoid content were found in ethanolic extracts 280.12 ± 2.04 mg quercetin equivalent/g of dry plant extract. The highest antioxidant activities were found for the ethanolic extract (IC 50 = 18 ± 0.90, 20 ± 0.24 g/mL) by DPPH and ABTS scavenging assays, respectively. Both extracts showed moderate inhibition of superoxide inhibition (O 2•− ) and nitric oxide (NO) production in concentration-dependent manner. Antibacterial activity was calculated by disk diffusion, minimum inhibitory concentration (MIC), and minimum bacterial concentration (MBC). The ethanolic extract had the greatest activity against methicillin resistant Staphylococcus aureus, methicillin sensitive S. aureus, and Streptococcus pyogenes with zone diameters of 11.67 ± 1.53, 10.33 ± 2.52, and 10.33 ± 1.15 mm, respectively. The corresponding MIC and MBCs were 5, 0.625, and 0.039 mg/mL. P. cablin extracts contain antioxidant and antibacterial properties that should be exploited for possible clinical application.

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“…In Soxhlet extraction method hot extraction process was used so that the extraction yield was high compared to maceration method. In addition, during the Soxhlet extractor method, the high molecular and less soluble antioxidant compounds extracted more due to recycle of the hot solvent [16] . However, in maceration method the yield of extraction was low, which might be due to cold extraction and extraction time.…”

Section: Discussionmentioning

confidence: 99%