Comparative Analysis of Chemical Constituents of Moringa oleifera Leaves from China and India by Ultra-Performance Liquid Chromatography Coupled with Quadrupole-Time-Of-Flight Mass Spectrometry

Abstract

With the aim to discuss the similarities and differences of phytochemicals in Moringa oleifera leaves collected from China (CML) and India (IML) in mind, comparative ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS) analysis was performed in this study. A screening analysis based on a UNIFI platform was first carried out to discuss the similarities. Next, untargeted metabolomic analysis based on multivariate statistical analysis was performed to discover the differences. As a result, a total of 122 components, containing 118 shared constituents, were characterized from CML and IML. The structure types included flavonoids, alkaloids, glyosides, organic acids and organic acid esters, iridoids, lignans, and steroids, etc. For CML, 121 compounds were characterized; among these, 18 potential biomarkers with higher contents enabled differentiation from IML. For IML, 119 compounds were characterized; among these, 12 potential biomarkers with higher contents enabled differentiation from CML. It could be concluded that both CML and IML are rich in phytochemicals and that CML is similar to IML in the kinds of the compounds it contains, except for the significant differences in the contents of some compounds. This comprehensive phytochemical profile study provides a basis for explaining the effect of different growth environments on secondary metabolites and exists as a reference for further research into or applications of CML in China.

Improvements of alveolar bone healing using Moringa oleifera leaf powder and extract biomimetic composite: an experimental study in dogs

“…10,12.13.18,19 For example, A study conducted on differences between chemical constitutes of MO leaf in China and India results showed some differences of ingredients. 6,10 From all above, quality control method is demanded for standardization, to access the active ingredients. Current study utilize dried leaf powder by conventional method as stated in material and method section to preserve as a much as possible the active ingredients and to be used for long time without loss of value.…”

“…Magnesium (Mg) affects the bone cells activities and bone homeostasis. [7][8][9][10] Most studies have focused on the leaves of the plant. Moringa oleifera leaf (MO) have been confirmed for antioxidant, anti-inflammatory, anticancer, and antimicrobial properties.…”

Comprehensive metabolomics analysis based on UPLC-Q/TOF-MSEand the anti-COPD effect of different parts ofCelastrus orbiculatusThunb.

“…by UNIFI platform UNIFI 1.7.0 soware (Waters, Manchester, UK) was used for data analysis. 28,29 Firstly, in addition to the internal Traditional Medicine Library on UNIFI platform, the chemical constituent investigation was conducted. As the result, a self-built database of chemical compounds isolated from the genus of Celastrus L. was established by searching the online databases including Web of Science, Medline, PubMed, ChemSpider and China National Knowledge Infrastructure (CNKI).…”

Metabolomics Reveals Ecological Significance of Secondary Metabolites in Scutellaria baicalensis under drought stress

“…The secondary metabolites in a plant are numerous, by Ultra-High-Performance Liquid Chromatography a total of 447 metabolites in Isatis indigotica Fortune, 128 in American ginseng root, 132 in Scutellaria baicalensis, 122 in Moringa oleifera leaves were identified [13][14][15][16] ,most of them are secondary metabolites. Their activities differ from each other, their proportion varied according to the changing environmental conditions [17] .…”