Metabolomics Reveals Ecological Significance of Secondary Metabolites in Scutellaria baicalensis under drought stress

Meng, Xiangcai; Wang, Bin; Du, Hongwei; Zhang, Aihua; Wang, Xijun

doi:10.21203/rs.3.rs-23642/v1

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…from the family Rutaceae rapidly biosynthesized two flavone compounds such as isovitexin and vitexin in response to drought stress ( Hu et al., 2021 ). In Scutellaria baicalensis roots, chrysin was accumulated in response to drought stress, and a high concentration of chrysin helps plants to eliminate the excessive ROS produced during drought stress and maintains the equilibrium in plant cells ( Meng et al., 2020 ). Our results showed that cynaroside, eriocitrin, 2′-hydroxygenistein, 2′-hydroxydaidzein, chrysin, neohesperidin, kaempferol 3-neohesperidoside, baimaside, hyperoside, quercitrin, and vitexin were uniquely higher in the leaves of sour orange after drought stress, whereas these compounds were significantly lower or had no obvious difference in pummelo and lemon under drought stress ( Figure 2 ; Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

LC-MS/MS-based metabolomics approach identified novel antioxidant flavonoids associated with drought tolerance in citrus species

et al. 2023

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Citrus fruits are cultivated around the world, and they face drought stress frequently during their growth and development. Previous studies showed that citrus plants biosynthesized flavonoid compounds in response to abiotic stress. In this study, we have quantified 37 flavonoid compounds from the leaves of three distinct citrus species including sour orange (drought-tolerant), pummelo ‘Majia you pummelo’ (drought-sensitive), and lemon (drought-sensitive). The 37 flavonoids consisted of 12 flavones, 10 flavonols, 6 flavanones, 5 isoflavanones, and 1 each for chalcone, flavanol, flavanonol, and flavone glycoside. Drought stress differentially altered the flavonoid metabolism in drought-tolerant and drought-sensitive citrus species. The kaempferol 3-neohesperidoside was 17-fold higher in sour orange (124.41 nmol/L) after 18 days of drought stress than lemon (7.33 nmol/L). In sour orange, neohesperidin (69.49 nmol/L) was 1,407- and 37-fold higher than pummelo and lemon, respectively. In sour orange, some flavonoids were significantly increased, such as vitexin, neohesperidin, cynaroside, hyperoside, genistin, kaempferol 3-neohesperidoside, eriocitrin, and luteolin, in response to drought stress, whereas in lemon, these flavonoids were significantly decreased or not altered significantly in response to drought stress. Moreover, the total contents of flavonoids and antioxidant activity were increased in sour orange as compared with pummelo and lemon. The genes associated with flavonoid biosynthesis (PAL, CHI, FLS, GT1, F3H, F3’M, C4H, 4CL, FLS, FG2, FG3, and CYP81E1) were more highly expressed in sour orange leaves than in pummelo and lemon after drought stress. These outcomes showed that pummelo and lemon failed to biosynthesize antioxidant flavonoids to cope with the prolonged drought stress, whereas the sour orange biosynthesized fortified flavonoid compounds with increased antioxidant activity to detoxify the harmful effects of reactive oxygen species produced during drought stress.

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Section: Discussionmentioning

confidence: 99%

LC-MS/MS-based metabolomics approach identified novel antioxidant flavonoids associated with drought tolerance in citrus species

et al. 2023

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“…Another study found that SOD was strongly expressed in the heart of white-leg shrimp (Penaeus vannamei), stimulated by ROS after one week of culture [58]. S. baicalensis decreased the ROS level and maintained the equilibrium of ROS through biosynthesis [59]. S. baicalensis Georgi flower extract (SFE) could significantly reduce oxidative damage of aging rats by increasing their SOD level in the serum [60].…”

Section: Discussionmentioning

confidence: 99%

Scutellaria Polysaccharide Mediates the Immunity and Antioxidant Capacity of the Giant Freshwater Prawn (Macrobrachium Rosenbergii)

Sun

Lin

Zhang

et al. 2021

Preprint

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Background: The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially valuable freshwater crustacean species that is cultivated throughout the world. M. rosenbergii is frequently affected by a variety of diseases. Therefore, feed additive research is necessary to improve the immunity and survival rate of M. rosenbergii. Scutellaria polysaccharide (SPS) extracted from Scutellaria baicalensis (a Chinese medicinal herb) can enhance the antioxidant ability of organisms. Methods: In this study, M. rosenbergii were fed with 50 mg/kg, 100 mg/kg, and 150 mg/kg of SPS. Following a four-week feeding trial, SPS had no positive effect on the growth of M. rosenbergii compared with the control group. Then, the immunity and antioxidant capacity of M. rosenbergii were tested by qRT-PCR and enzyme activities. Results: The results showed that the expressions of prophenoloxidase (proPO) and toll receptor (Toll-R) mRNA showed no changes in hemocytes of M. rosenbergii. However, the expressions of heat shock protein 70 (HSP70) and nuclear factor κB (NF-κB) mRNA were up-regulated during the first two weeks of culture and were down-regulated in weeks 3 and 4. The mRNA expressions of HSP70, NF-κB, and Toll-R (participating in the immune response) in heart, muscle, and hepatopancreas were decreased after four weeks of SPS feeding. This indicated that long-term feeding of SPS could regulate the immune responses of M. rosenbergii. The activity levels of antioxidant biomarkers, alkaline phosphatase (ACP), acid phosphatase (AKP), polyphenol oxidase (PPO), catalase (CAT), and superoxide dismutase (SOD), in hemocytes, heart, muscle, and hepatopancreas increased during the feeding time, indicating that the antioxidant capacity of M. rosenbergii was improved by SPS supplementation in the feed. Conclusions: In summary, SPS was conducive to enhancing the antioxidant capacity of M. rosenbergii. These results provide a theoretical basis in supporting of SPS addition to the feed of M. rosenbergii.

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Section: Phosphatase Can Catalyze the Hydrolysis Of Various Phosphoro...mentioning

confidence: 99%

Scutellaria polysaccharide mediates the immunity and antioxidant capacity of giant freshwater prawn (Macrobrachium rosenbergii)

Sun

Lin

Sun

et al. 2023

Developmental & Comparative Immunology

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Metabolomics Reveals Ecological Significance of Secondary Metabolites in Scutellaria baicalensis under drought stress

Abstract: Background

Cited by 3 publications

References 19 publications

LC-MS/MS-based metabolomics approach identified novel antioxidant flavonoids associated with drought tolerance in citrus species

LC-MS/MS-based metabolomics approach identified novel antioxidant flavonoids associated with drought tolerance in citrus species

Scutellaria Polysaccharide Mediates the Immunity and Antioxidant Capacity of the Giant Freshwater Prawn (Macrobrachium Rosenbergii)

Scutellaria polysaccharide mediates the immunity and antioxidant capacity of giant freshwater prawn (Macrobrachium rosenbergii)

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