Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering

Wang, Lina; Johnson, Joshua A.; Zhang, Qixu; Beahm, Elisabeth K.

doi:10.1016/j.actbio.2013.06.035

Cited by 136 publications

(131 citation statements)

References 36 publications

(52 reference statements)

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“…Although decellularized adipose matrix holds tremendous potential for adipose tissue regeneration, recent attempts to engineer adipose tissue have involved only decellularized adipose tissue matrix without the appropriate circulatory network [15, 18, 19]. Consequently, the engineered constructs require lengthy periods to achieve neovascularization and integration with the host tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Immediately after harvest, the femoral artery was catheterized with a 24G catheter (BD Biosciences, San Jose, CA), and the flap was irrigated with normal saline through the femoral artery until only clear normal saline flowed from the femoral vein. The flaps were frozen at −80°C and thawed at room temperature for 3 cycles and were then processed with chemical detergents as described previously [15]. For each flap, the artery was catheterized, and the flap was connected via the catheter to a Masterflex pump perfusion system (Cole-Parmer, Vernon Hills, IL) and perfused with ultrapure water (2 ml/min) for 1 day at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…(These tissues are usually discarded after surgery.) hASCs were cultured using our established protocol [15]. hASCs were confirmed by adult stem cell differentiation assays (GIBCO stem cell differentiation kit, Life Technologies).…”

Section: Methodsmentioning

confidence: 99%

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Decellularized skin/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps

et al. 2016

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Using a perfusion decellularization protocol, we developed a decellularized skin/adipose tissue flap (DSAF) comprising extracellular matrix (ECM) and intact vasculature. Our DSAF had a dominant vascular pedicle, microcirculatory vascularity, and a sensory nerve network and retained three-dimensional (3D) nanofibrous structures well. DSAF, which was composed of collagen and laminin with well-preserved growth factors (e.g., vascular endothelial growth factor, basic fibroblast growth factor), was successfully repopulated with human adipose-derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs), which integrated with DSAF and formed 3D aggregates and vessel-like structures in vitro. We used microsurgery techniques to re-anastomose the recellularized DSAF into nude rats. In vivo, the engineered flap construct underwent neovascularization and constructive remodeling, which was characterized by the predominant infiltration of M2 macrophages and significant adipose tissue formation at 3 months postoperatively. Our results indicate that DSAF co-cultured with hASCs and HUVECs is a promising platform for vascularized soft tissue flap engineering. This platform is not limited by the flap size, as the entire construct can be immediately perfused by the recellularized vascular network following simple re-integration into the host using conventional microsurgical techniques.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Decellularized skin/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps

et al. 2016

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“…For instance adipose stem cells (ASCs) are a great promise for regenerative medicine applications. The use of de-cellularized human adipose tissue ECM combined with ASCs is a strategy that can be employed in the tissue engineering area [55]. Kim and collaborators designed a free-cell scaffold for adipose tissue regeneration; the aim was creating a speciic scafold to recruit cells into a desire cell type [56].Hence, research on adipocyte biomechanics has potential for evidence that could be applied to the development of methods for tissue construct.…”

Section: Additional Considerations About Cell Biomechanics: the Case mentioning

confidence: 99%

Mechanical Stimulation of Cells Through Scaffold Design for Tissue Engineering

Oliver-Urrutia¹,

García²,

Estrada³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Tissue engineering scafolds atempt to mimic the stem cell environment by creating different biophysical and chemical signals. On the other hand, stem cells are able to sense these characteristics and change their destiny. Scientists try to explain these phenomena through scafold design and in vitro experiments, but the mechanisms implicated remain unclear. Moreover, environment-cell interactions are a key process to get organs and tissues arrangement. Therefore, this chapter deals with the mechanical signals and mechanism involved in cell behaviour through scafolds as a strategy in tissue engineering.

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“…DECM scaffolds improve cell growth, migration, differentiation and can positively influence tissue repair and remodeling [3][4][5][6][7]. DECM from a variety of tissues such as skeletal muscle [8][9][10], brain [11,12], urinary bladder [2,5,13], small intestinal submucosa [14][15][16], liver [4,[17][18][19], skin/adipose tissue [20][21][22], blood vessels [23][24][25], heart valves [26,27] and tendons [28] have been used for tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

Decellularized extracellular matrices for tissue engineering applications

Elmashhady¹,

Kraemer²,

Patel³

et al. 2017

Electrospinning

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Decellularization removes cellular antigens while preserving the ultrastructure and composition of extracellular matrix (ECM). Decellularized ECM (DECM) scaffolds have been widely used in various tissue engineering applications with varying levels of success. The mechanical, architectural and bioactive properties of a DECM scaffold depend largely on the method of decellularization and dictate its clinical efficacy. This article highlights the advantages and challenges associated with the clinical use of DECM scaffolds. Poor mechanical strength is a significant disadvantage of some DECM scaffolds in the repair of load-bearing tissues as well as critical-size defects, where long-term mechanical support is required for the regenerating tissue. Combining DECM scaffolds with synthetic biocompatible polymers could provide a useful strategy to circumvent the issues of poor mechanical stability. This article reviews studies that have combined DECM scaffolds from various tissues with synthetic polymers to create hybrid scaffolds using electrospinning. These hybrid scaffolds provide a mechanical backbone while retaining the bioactive properties of DECM, thus offering a significant advantage for tissue engineering and regenerative medicine applications.

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Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering

Cited by 136 publications

References 36 publications

Decellularized skin/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps

Decellularized skin/adipose tissue flap matrix for engineering vascularized composite soft tissue flaps

Mechanical Stimulation of Cells Through Scaffold Design for Tissue Engineering

Decellularized extracellular matrices for tissue engineering applications

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